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Inter-varietal variation in the composition, physicochemical properties and antioxidant activity of winter melon [Benincasa hispida (Thunb.) Cogn.] fruit seeds and seed oils

The proximate composition of seeds and the physicochemical properties and antioxidant activity of extracted seed oils from two Malaysian winter melon cultivars namely round (Cultivar 1) and hybrid (Cultivar 2) were examined. The seeds from Cultivar 1 and Cultivar 2 exhibited protein, total dietar...

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Main Author: Mohammad, Nor Azizah
Format: Thesis
Language:English
Published: 2015
Online Access:http://psasir.upm.edu.my/id/eprint/67786/1/FSTM%202015%2039%20IR.pdf
http://psasir.upm.edu.my/id/eprint/67786/
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spelling my.upm.eprints.677862019-03-29T04:11:01Z http://psasir.upm.edu.my/id/eprint/67786/ Inter-varietal variation in the composition, physicochemical properties and antioxidant activity of winter melon [Benincasa hispida (Thunb.) Cogn.] fruit seeds and seed oils Mohammad, Nor Azizah The proximate composition of seeds and the physicochemical properties and antioxidant activity of extracted seed oils from two Malaysian winter melon cultivars namely round (Cultivar 1) and hybrid (Cultivar 2) were examined. The seeds from Cultivar 1 and Cultivar 2 exhibited protein, total dietary fiber, carbohydrate, moisture and ash contents 18.26-20.45, 41.55-46.30, 1.70-5.78, 6.47-7.03, and 3.73-7.42%, respectively. The oils were extracted based on Soxhlet method by petroleum ether, whilst yields ranged from 17.78-23.55% (wt/wt). The extracted oils were analyzed for physicochemical parameters, fatty acids, tocopherols profiles, phenolic compound, and antioxidant activities. The physicochemical properties showed ranges for specific gravity (25°C), refractive index (nD 25°C), iodine value (IV), saponification value (SV), peroxide value (PV), and free fatty acid (% as oleic acid) as 0.88-0.91, 1.4627- 1.4630, 119.9-125.1 g I/100 g oil, 182.3-194.1 mg KOH/ g oil, 1.13-1.27 mequiv.O2/kg, and 1.57-1.87%, respectively. The oil color intensity in terms of yellow and red units was 6.9Y + 1.0 R to 8.9Y + 1.9R. Linoleic acid (C18:2) was established to be the principal fatty acid (63.10-70.64%) followed by C16:0 (12.45-17.59%), C18:1 (8.46-12.73%) and C18:0 (5.13-6.26%). The predominant tocopherols were β- tocopherol, followed by γ-tocopherol, δ-tocopherol, γ-tocotrienol, and α-tocopherol, respectively. Vanillin was the principal compound in both seed oils ranging from 38.9 to 51.4% of the total phenolic compound (12.27-17.25 μg/g), including gallic acid, protocatechuic acid, 3,4-dihydroxybenzaldehyde, vanillic acid, para-coumaric acid, trans-cinnamic acid and ferulic acid. Three solvent systems namely ethanol, methanol, and isopropanol at two different concentrations (80% and 100%) were used as extraction solvents for assessment of antioxidant activity and total phenolics content (TPC) of the oils. The results revealed the notable effect of extraction solvents. TPC of oil extracts were ranged from 19.37 to 203.93 μg GAE/g oil. The highest trolox equivalent antioxidant capacity (TEAC) among oil extracts was found to be not significant between two cultivars. Most of oil extracts from Cultivar 2 showed significantly higher TPC and antioxidant activity in terms of IC50 value (DPPH radical), compared to Cultivar 1. The data of this study support that winter melon seed oils of the tested cultivars could be a dietary source of tocochromanol, phenolic compound, and natural antioxidants. 2015-03 Thesis NonPeerReviewed text en http://psasir.upm.edu.my/id/eprint/67786/1/FSTM%202015%2039%20IR.pdf Mohammad, Nor Azizah (2015) Inter-varietal variation in the composition, physicochemical properties and antioxidant activity of winter melon [Benincasa hispida (Thunb.) Cogn.] fruit seeds and seed oils. Masters thesis, Universiti Putra Malaysia.
institution Universiti Putra Malaysia
building UPM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Putra Malaysia
content_source UPM Institutional Repository
url_provider http://psasir.upm.edu.my/
language English
description The proximate composition of seeds and the physicochemical properties and antioxidant activity of extracted seed oils from two Malaysian winter melon cultivars namely round (Cultivar 1) and hybrid (Cultivar 2) were examined. The seeds from Cultivar 1 and Cultivar 2 exhibited protein, total dietary fiber, carbohydrate, moisture and ash contents 18.26-20.45, 41.55-46.30, 1.70-5.78, 6.47-7.03, and 3.73-7.42%, respectively. The oils were extracted based on Soxhlet method by petroleum ether, whilst yields ranged from 17.78-23.55% (wt/wt). The extracted oils were analyzed for physicochemical parameters, fatty acids, tocopherols profiles, phenolic compound, and antioxidant activities. The physicochemical properties showed ranges for specific gravity (25°C), refractive index (nD 25°C), iodine value (IV), saponification value (SV), peroxide value (PV), and free fatty acid (% as oleic acid) as 0.88-0.91, 1.4627- 1.4630, 119.9-125.1 g I/100 g oil, 182.3-194.1 mg KOH/ g oil, 1.13-1.27 mequiv.O2/kg, and 1.57-1.87%, respectively. The oil color intensity in terms of yellow and red units was 6.9Y + 1.0 R to 8.9Y + 1.9R. Linoleic acid (C18:2) was established to be the principal fatty acid (63.10-70.64%) followed by C16:0 (12.45-17.59%), C18:1 (8.46-12.73%) and C18:0 (5.13-6.26%). The predominant tocopherols were β- tocopherol, followed by γ-tocopherol, δ-tocopherol, γ-tocotrienol, and α-tocopherol, respectively. Vanillin was the principal compound in both seed oils ranging from 38.9 to 51.4% of the total phenolic compound (12.27-17.25 μg/g), including gallic acid, protocatechuic acid, 3,4-dihydroxybenzaldehyde, vanillic acid, para-coumaric acid, trans-cinnamic acid and ferulic acid. Three solvent systems namely ethanol, methanol, and isopropanol at two different concentrations (80% and 100%) were used as extraction solvents for assessment of antioxidant activity and total phenolics content (TPC) of the oils. The results revealed the notable effect of extraction solvents. TPC of oil extracts were ranged from 19.37 to 203.93 μg GAE/g oil. The highest trolox equivalent antioxidant capacity (TEAC) among oil extracts was found to be not significant between two cultivars. Most of oil extracts from Cultivar 2 showed significantly higher TPC and antioxidant activity in terms of IC50 value (DPPH radical), compared to Cultivar 1. The data of this study support that winter melon seed oils of the tested cultivars could be a dietary source of tocochromanol, phenolic compound, and natural antioxidants.
format Thesis
author Mohammad, Nor Azizah
spellingShingle Mohammad, Nor Azizah
Inter-varietal variation in the composition, physicochemical properties and antioxidant activity of winter melon [Benincasa hispida (Thunb.) Cogn.] fruit seeds and seed oils
author_facet Mohammad, Nor Azizah
author_sort Mohammad, Nor Azizah
title Inter-varietal variation in the composition, physicochemical properties and antioxidant activity of winter melon [Benincasa hispida (Thunb.) Cogn.] fruit seeds and seed oils
title_short Inter-varietal variation in the composition, physicochemical properties and antioxidant activity of winter melon [Benincasa hispida (Thunb.) Cogn.] fruit seeds and seed oils
title_full Inter-varietal variation in the composition, physicochemical properties and antioxidant activity of winter melon [Benincasa hispida (Thunb.) Cogn.] fruit seeds and seed oils
title_fullStr Inter-varietal variation in the composition, physicochemical properties and antioxidant activity of winter melon [Benincasa hispida (Thunb.) Cogn.] fruit seeds and seed oils
title_full_unstemmed Inter-varietal variation in the composition, physicochemical properties and antioxidant activity of winter melon [Benincasa hispida (Thunb.) Cogn.] fruit seeds and seed oils
title_sort inter-varietal variation in the composition, physicochemical properties and antioxidant activity of winter melon [benincasa hispida (thunb.) cogn.] fruit seeds and seed oils
publishDate 2015
url http://psasir.upm.edu.my/id/eprint/67786/1/FSTM%202015%2039%20IR.pdf
http://psasir.upm.edu.my/id/eprint/67786/
_version_ 1643839008590004224
score 13.211869

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