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Upregulation of cholesterol storage signalling genes in HEPG2 cells supplemented with edible bird nest (EBN) extract

“Caviar of the East” or better known as Edible-Bird Nest (EBN) is a highly valuable product composed of solidified salivary secretion of a few insectivorous swiftlet species. Swiftlets falls under the family Apodidae and the two main genera with the highest commercial values are Aerodramus (echolati...

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Main Authors: Tawai, Darreen, Noor, M. A. M., Salleh, F., Razak, I. S., Mansor, R., Ideris, A., Md. Ajat, Mohd Mokrish
Format: Conference or Workshop Item
Language:English
Published: Faculty of Veterinary Medicine, Universiti Putra Malaysia 2018
Online Access:http://psasir.upm.edu.my/id/eprint/67655/1/WPSA_2018-18.pdf
http://psasir.upm.edu.my/id/eprint/67655/
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spelling my.upm.eprints.676552019-04-09T03:48:52Z http://psasir.upm.edu.my/id/eprint/67655/ Upregulation of cholesterol storage signalling genes in HEPG2 cells supplemented with edible bird nest (EBN) extract Tawai, Darreen Noor, M. A. M. Salleh, F. Razak, I. S. Mansor, R. Ideris, A. Md. Ajat, Mohd Mokrish “Caviar of the East” or better known as Edible-Bird Nest (EBN) is a highly valuable product composed of solidified salivary secretion of a few insectivorous swiftlet species. Swiftlets falls under the family Apodidae and the two main genera with the highest commercial values are Aerodramus (echolating swiftlets) and Collocalia (non-echolating swiftlets). The objective of this study is to determine the qualitative genes expression level associated in lipid signalling pathways such as Acetyl-CoA acetyltransferase 2 (ACAT2), Diacylglycerol O-acyltransferase 2 (DGAT2), Low density lipoprotein receptor (LDLR), Sterol regulatory element binding transcription factor 2 (SREBP2), 3-hydroxy-3-methylglutaryl-CoA reductase (HMGCR), Proprotein Convertase Subtilisin/kexin type 9 (PCSK9) in HepG2 mammalian cell with or without supplementation of EBN extract and exogenous lipid. Raw EBN were manually cleaned, dried in 60°C oven and left overnight. In this project, EBN supernatant were added with acetone using 1:2 ratio and kept in -80°C for an hour. HepG2 mammalian cells were cultured in six wells plates and each wells were treated according to Base Control, Positive Control, Negative Control, Treatment 1, Treatment 2 and Treatment 3. In this study, there were significant differences in terms of genes expression especially in ACAT2 and LDLR genes with supplementation of EBN extract and exogenous lipid concentrate. Faculty of Veterinary Medicine, Universiti Putra Malaysia 2018 Conference or Workshop Item PeerReviewed text en http://psasir.upm.edu.my/id/eprint/67655/1/WPSA_2018-18.pdf Tawai, Darreen and Noor, M. A. M. and Salleh, F. and Razak, I. S. and Mansor, R. and Ideris, A. and Md. Ajat, Mohd Mokrish (2018) Upregulation of cholesterol storage signalling genes in HEPG2 cells supplemented with edible bird nest (EBN) extract. In: 3rd World's Poultry Science Association (Malaysia Branch) and World Veterinary Poultry Association (Malaysia Branch) Scientific Conference 2018, 18-19 Apr. 2018, Faculty of Veterinary Medicine, Universiti Putra Malaysia and Kuala Lumpur Convention Centre, Kuala Lumpur. (pp. 64-65).
institution Universiti Putra Malaysia
building UPM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Putra Malaysia
content_source UPM Institutional Repository
url_provider http://psasir.upm.edu.my/
language English
description “Caviar of the East” or better known as Edible-Bird Nest (EBN) is a highly valuable product composed of solidified salivary secretion of a few insectivorous swiftlet species. Swiftlets falls under the family Apodidae and the two main genera with the highest commercial values are Aerodramus (echolating swiftlets) and Collocalia (non-echolating swiftlets). The objective of this study is to determine the qualitative genes expression level associated in lipid signalling pathways such as Acetyl-CoA acetyltransferase 2 (ACAT2), Diacylglycerol O-acyltransferase 2 (DGAT2), Low density lipoprotein receptor (LDLR), Sterol regulatory element binding transcription factor 2 (SREBP2), 3-hydroxy-3-methylglutaryl-CoA reductase (HMGCR), Proprotein Convertase Subtilisin/kexin type 9 (PCSK9) in HepG2 mammalian cell with or without supplementation of EBN extract and exogenous lipid. Raw EBN were manually cleaned, dried in 60°C oven and left overnight. In this project, EBN supernatant were added with acetone using 1:2 ratio and kept in -80°C for an hour. HepG2 mammalian cells were cultured in six wells plates and each wells were treated according to Base Control, Positive Control, Negative Control, Treatment 1, Treatment 2 and Treatment 3. In this study, there were significant differences in terms of genes expression especially in ACAT2 and LDLR genes with supplementation of EBN extract and exogenous lipid concentrate.
format Conference or Workshop Item
author Tawai, Darreen
Noor, M. A. M.
Salleh, F.
Razak, I. S.
Mansor, R.
Ideris, A.
Md. Ajat, Mohd Mokrish
spellingShingle Tawai, Darreen
Noor, M. A. M.
Salleh, F.
Razak, I. S.
Mansor, R.
Ideris, A.
Md. Ajat, Mohd Mokrish
Upregulation of cholesterol storage signalling genes in HEPG2 cells supplemented with edible bird nest (EBN) extract
author_facet Tawai, Darreen
Noor, M. A. M.
Salleh, F.
Razak, I. S.
Mansor, R.
Ideris, A.
Md. Ajat, Mohd Mokrish
author_sort Tawai, Darreen
title Upregulation of cholesterol storage signalling genes in HEPG2 cells supplemented with edible bird nest (EBN) extract
title_short Upregulation of cholesterol storage signalling genes in HEPG2 cells supplemented with edible bird nest (EBN) extract
title_full Upregulation of cholesterol storage signalling genes in HEPG2 cells supplemented with edible bird nest (EBN) extract
title_fullStr Upregulation of cholesterol storage signalling genes in HEPG2 cells supplemented with edible bird nest (EBN) extract
title_full_unstemmed Upregulation of cholesterol storage signalling genes in HEPG2 cells supplemented with edible bird nest (EBN) extract
title_sort upregulation of cholesterol storage signalling genes in hepg2 cells supplemented with edible bird nest (ebn) extract
publisher Faculty of Veterinary Medicine, Universiti Putra Malaysia
publishDate 2018
url http://psasir.upm.edu.my/id/eprint/67655/1/WPSA_2018-18.pdf
http://psasir.upm.edu.my/id/eprint/67655/
_version_ 1643838969725583360
score 13.211869

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