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Biocontrol of fusarium ear rot of maize using Trichoderma species

The present study conducted to screen the biocontrol agent of different species of Trichoderma against Fusarium ear rot (FER), which examined under in vitro and plant house conditions. Seventy-two isolates of Trichoderma were successfully isolated from rhizosphere of different crops. All isolates we...

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Main Author: Salleh, Suhaida
Format: Thesis
Language:English
Published: 2013
Online Access:http://psasir.upm.edu.my/id/eprint/67400/1/FS%202013%2089%20IR.pdf
http://psasir.upm.edu.my/id/eprint/67400/
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spelling my.upm.eprints.674002019-03-14T06:54:39Z http://psasir.upm.edu.my/id/eprint/67400/ Biocontrol of fusarium ear rot of maize using Trichoderma species Salleh, Suhaida The present study conducted to screen the biocontrol agent of different species of Trichoderma against Fusarium ear rot (FER), which examined under in vitro and plant house conditions. Seventy-two isolates of Trichoderma were successfully isolated from rhizosphere of different crops. All isolates were identified into three species such as T. harzianum (51 isolates), T. koningii (20 isolates) and T. hamatum (1 isolate). Pathogenicity test of six isolates of Fusarium (B84c, C116c, C121c, P175c, P191c and P202c) to maize ear (variety Thailand Supersweet) and the symptoms development of FER were observed after seven days of pathogens inoculation. The results proved that F. proliferatum P202c and F. verticillioides C116c were pathogenic and caused FER. Both pathogens were challenged in dual culture test with 72 identified isolates of Trichoderma. Trichoderma harzianum T73s gave the highest percentage inhibition of 73.10% and 79.46% against F. proliferatum P202c and F. verticillioides C116c, respectively. The interaction appeared with the formation of clear zone between the pathogens and Trichoderma. Trichoderma harzianum T73s was further tested for its efficacy to suppress FER disease under plant house condition. The severity of FER was significantly reduced in the maize plants treated with T. harzianum T73s every week, immediately after planting (T1) with percentage of disease severity (DS) 10% and 8% against P202c and C116c, respectively. In contrast, the DS of positive control (C3) for P202c and C116c were 95% and 94%, respectively. The ears in T2 moderately suppressed FER with DS 38% and 34% against P202c and C116c, respectively. The ears for negative control that were inoculated with distilled water and uninoculated remained intact. The other treatments (T3 and T4) were unable to reduce the severity of FER disease. This study reported that T. harzianum T73s can be applied as a biocontrol agent and has a potential for further tests in the field and commercial scales. 2013-10 Thesis NonPeerReviewed text en http://psasir.upm.edu.my/id/eprint/67400/1/FS%202013%2089%20IR.pdf Salleh, Suhaida (2013) Biocontrol of fusarium ear rot of maize using Trichoderma species. Masters thesis, Universiti Putra Malaysia.
institution Universiti Putra Malaysia
building UPM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Putra Malaysia
content_source UPM Institutional Repository
url_provider http://psasir.upm.edu.my/
language English
description The present study conducted to screen the biocontrol agent of different species of Trichoderma against Fusarium ear rot (FER), which examined under in vitro and plant house conditions. Seventy-two isolates of Trichoderma were successfully isolated from rhizosphere of different crops. All isolates were identified into three species such as T. harzianum (51 isolates), T. koningii (20 isolates) and T. hamatum (1 isolate). Pathogenicity test of six isolates of Fusarium (B84c, C116c, C121c, P175c, P191c and P202c) to maize ear (variety Thailand Supersweet) and the symptoms development of FER were observed after seven days of pathogens inoculation. The results proved that F. proliferatum P202c and F. verticillioides C116c were pathogenic and caused FER. Both pathogens were challenged in dual culture test with 72 identified isolates of Trichoderma. Trichoderma harzianum T73s gave the highest percentage inhibition of 73.10% and 79.46% against F. proliferatum P202c and F. verticillioides C116c, respectively. The interaction appeared with the formation of clear zone between the pathogens and Trichoderma. Trichoderma harzianum T73s was further tested for its efficacy to suppress FER disease under plant house condition. The severity of FER was significantly reduced in the maize plants treated with T. harzianum T73s every week, immediately after planting (T1) with percentage of disease severity (DS) 10% and 8% against P202c and C116c, respectively. In contrast, the DS of positive control (C3) for P202c and C116c were 95% and 94%, respectively. The ears in T2 moderately suppressed FER with DS 38% and 34% against P202c and C116c, respectively. The ears for negative control that were inoculated with distilled water and uninoculated remained intact. The other treatments (T3 and T4) were unable to reduce the severity of FER disease. This study reported that T. harzianum T73s can be applied as a biocontrol agent and has a potential for further tests in the field and commercial scales.
format Thesis
author Salleh, Suhaida
spellingShingle Salleh, Suhaida
Biocontrol of fusarium ear rot of maize using Trichoderma species
author_facet Salleh, Suhaida
author_sort Salleh, Suhaida
title Biocontrol of fusarium ear rot of maize using Trichoderma species
title_short Biocontrol of fusarium ear rot of maize using Trichoderma species
title_full Biocontrol of fusarium ear rot of maize using Trichoderma species
title_fullStr Biocontrol of fusarium ear rot of maize using Trichoderma species
title_full_unstemmed Biocontrol of fusarium ear rot of maize using Trichoderma species
title_sort biocontrol of fusarium ear rot of maize using trichoderma species
publishDate 2013
url http://psasir.upm.edu.my/id/eprint/67400/1/FS%202013%2089%20IR.pdf
http://psasir.upm.edu.my/id/eprint/67400/
_version_ 1643838893363036160
score 13.211869

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