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Loop mediated isothermal amplification; a review on its application and strategy in animal species authentication of meat based food products

Isothermal amplification is a technique that can amplify target DNA sequences at a single incubation temperature. Loop-mediated isothermal amplification (LAMP) is an extension of the isothermal DNA amplification technique that combines rapidity, simplicity, high specificity and sensitivity. Due to i...

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Main Authors:	Tasrip, Nor Asmara, Khairil Mokhtar, Nur Fadhilah, Hanapi, Ummi Kalthum, Abdul Manaf, Yanty Noorzianna, Ali, Md. Eaqub, Cheah, Yoke Kqueen, Mustafa, Shuhaimi, Mohd Desa, Mohd Nasir
Format:	Article
Language:	English
Published:	Faculty of Food Science and Technology, Universiti Putra Malaysia 2019
Online Access:	http://psasir.upm.edu.my/id/eprint/67287/1/%281%29.pdf http://psasir.upm.edu.my/id/eprint/67287/ http://www.ifrj.upm.edu.my/26%20(01)%202019/(1).pdf
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id	my.upm.eprints.67287
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spelling	my.upm.eprints.672872019-04-08T08:20:34Z http://psasir.upm.edu.my/id/eprint/67287/ Loop mediated isothermal amplification; a review on its application and strategy in animal species authentication of meat based food products Tasrip, Nor Asmara Khairil Mokhtar, Nur Fadhilah Hanapi, Ummi Kalthum Abdul Manaf, Yanty Noorzianna Ali, Md. Eaqub Cheah, Yoke Kqueen Mustafa, Shuhaimi Mohd Desa, Mohd Nasir Isothermal amplification is a technique that can amplify target DNA sequences at a single incubation temperature. Loop-mediated isothermal amplification (LAMP) is an extension of the isothermal DNA amplification technique that combines rapidity, simplicity, high specificity and sensitivity. Due to its overwhelming characteristics, LAMP has been explored for its feasibility in detecting various subjects, and recently in meat-based food products for DNA-based meat species authentication. It has been developed to target various meat species such as porcine, chicken, horse, and ostrich with sensitivity as low as 0.1 pg/μL. Further improvement with the use of magnetic beads, electrochemiluminescence and special dye such as calcein and crystal violet had increased the sensitivity of the LAMP assay. Other important characteristics were specific target gene primers as well as a shorter incubation time, warranting a good prospect for rapid testing authentication. Faculty of Food Science and Technology, Universiti Putra Malaysia 2019 Article PeerReviewed text en http://psasir.upm.edu.my/id/eprint/67287/1/%281%29.pdf Tasrip, Nor Asmara and Khairil Mokhtar, Nur Fadhilah and Hanapi, Ummi Kalthum and Abdul Manaf, Yanty Noorzianna and Ali, Md. Eaqub and Cheah, Yoke Kqueen and Mustafa, Shuhaimi and Mohd Desa, Mohd Nasir (2019) Loop mediated isothermal amplification; a review on its application and strategy in animal species authentication of meat based food products. International Food Research Journal, 26 (1). pp. 1-10. ISSN 1985-4668; ESSN: 2231-7546 http://www.ifrj.upm.edu.my/26%20(01)%202019/(1).pdf
institution	Universiti Putra Malaysia
building	UPM Library
collection	Institutional Repository
continent	Asia
country	Malaysia
content_provider	Universiti Putra Malaysia
content_source	UPM Institutional Repository
url_provider	http://psasir.upm.edu.my/
language	English
description	Isothermal amplification is a technique that can amplify target DNA sequences at a single incubation temperature. Loop-mediated isothermal amplification (LAMP) is an extension of the isothermal DNA amplification technique that combines rapidity, simplicity, high specificity and sensitivity. Due to its overwhelming characteristics, LAMP has been explored for its feasibility in detecting various subjects, and recently in meat-based food products for DNA-based meat species authentication. It has been developed to target various meat species such as porcine, chicken, horse, and ostrich with sensitivity as low as 0.1 pg/μL. Further improvement with the use of magnetic beads, electrochemiluminescence and special dye such as calcein and crystal violet had increased the sensitivity of the LAMP assay. Other important characteristics were specific target gene primers as well as a shorter incubation time, warranting a good prospect for rapid testing authentication.
format	Article
author	Tasrip, Nor Asmara Khairil Mokhtar, Nur Fadhilah Hanapi, Ummi Kalthum Abdul Manaf, Yanty Noorzianna Ali, Md. Eaqub Cheah, Yoke Kqueen Mustafa, Shuhaimi Mohd Desa, Mohd Nasir
spellingShingle	Tasrip, Nor Asmara Khairil Mokhtar, Nur Fadhilah Hanapi, Ummi Kalthum Abdul Manaf, Yanty Noorzianna Ali, Md. Eaqub Cheah, Yoke Kqueen Mustafa, Shuhaimi Mohd Desa, Mohd Nasir Loop mediated isothermal amplification; a review on its application and strategy in animal species authentication of meat based food products
author_facet	Tasrip, Nor Asmara Khairil Mokhtar, Nur Fadhilah Hanapi, Ummi Kalthum Abdul Manaf, Yanty Noorzianna Ali, Md. Eaqub Cheah, Yoke Kqueen Mustafa, Shuhaimi Mohd Desa, Mohd Nasir
author_sort	Tasrip, Nor Asmara
title	Loop mediated isothermal amplification; a review on its application and strategy in animal species authentication of meat based food products
title_short	Loop mediated isothermal amplification; a review on its application and strategy in animal species authentication of meat based food products
title_full	Loop mediated isothermal amplification; a review on its application and strategy in animal species authentication of meat based food products
title_fullStr	Loop mediated isothermal amplification; a review on its application and strategy in animal species authentication of meat based food products
title_full_unstemmed	Loop mediated isothermal amplification; a review on its application and strategy in animal species authentication of meat based food products
title_sort	loop mediated isothermal amplification; a review on its application and strategy in animal species authentication of meat based food products
publisher	Faculty of Food Science and Technology, Universiti Putra Malaysia
publishDate	2019
url	http://psasir.upm.edu.my/id/eprint/67287/1/%281%29.pdf http://psasir.upm.edu.my/id/eprint/67287/ http://www.ifrj.upm.edu.my/26%20(01)%202019/(1).pdf
_version_	1643838859494031360
score	13.211869

Loop mediated isothermal amplification; a review on its application and strategy in animal species authentication of meat based food products

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