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Histological assesment of low temperature epidermal tissue damage in skin graft cryopreserved with antarctic yeast isolated antifreeze peptides

Sub-zero injuries in living tissue as a result of re-crystallization phenomenon is the major obstacle and complicates the application of cryopreserved tissues destined for transplantation. Understanding the freezing and thawing response of tissue against the sub-zero temperature would provide better...

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Main Authors: Khan, Muhammad Shoaib, Abubakar, Adamu Abdul, Abdul Rahman, Mohd Basyaruddin, Abu Bakar @ Zakaria, Md Zuki, Mohamed Mustapha, Noordin, Mohamad Yusof, Loqman
Format: Conference or Workshop Item
Language:English
Published: 2015
Online Access:http://psasir.upm.edu.my/id/eprint/67186/1/6th%20PCVC%20AND%2027th%20VAM-13.pdf
http://psasir.upm.edu.my/id/eprint/67186/
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spelling my.upm.eprints.671862019-03-08T06:25:50Z http://psasir.upm.edu.my/id/eprint/67186/ Histological assesment of low temperature epidermal tissue damage in skin graft cryopreserved with antarctic yeast isolated antifreeze peptides Khan, Muhammad Shoaib Abubakar, Adamu Abdul Abdul Rahman, Mohd Basyaruddin Abu Bakar @ Zakaria, Md Zuki Mohamed Mustapha, Noordin Mohamad Yusof, Loqman Sub-zero injuries in living tissue as a result of re-crystallization phenomenon is the major obstacle and complicates the application of cryopreserved tissues destined for transplantation. Understanding the freezing and thawing response of tissue against the sub-zero temperature would provide better understanding in the application of the non-toxic preservation technique of living tissues. The aim of this study was to evaluate the potential of antifreeze peptides (AFP) as a cryopreservative agent for living tissue e.g. skin. To determine the effects of cryopreservation on the tissue using AFP, type 1 AFP (peptide 1 m) was used to cryopreserve skin graft in different concentrations: 0.5, 1.0 and 2.0 mg/ml at -10°C or -20°C for 24 hrs. The histological distortions effects of the tissues were measured using a scoring system to determine the extent of freezing damage incurred in cryopreserved skin tissues. It was found that relatively less microscopic tissue damages occurred at -10°C compared to -20°C at higher AFP concentration. It is concluded that skin tissue cryopreservation could maintain comparatively better microscopic tissue integrity at -10°C using 2 mg/ml concentration of AFP type 1 as the cryopreservative agent. 2015 Conference or Workshop Item PeerReviewed text en http://psasir.upm.edu.my/id/eprint/67186/1/6th%20PCVC%20AND%2027th%20VAM-13.pdf Khan, Muhammad Shoaib and Abubakar, Adamu Abdul and Abdul Rahman, Mohd Basyaruddin and Abu Bakar @ Zakaria, Md Zuki and Mohamed Mustapha, Noordin and Mohamad Yusof, Loqman (2015) Histological assesment of low temperature epidermal tissue damage in skin graft cryopreserved with antarctic yeast isolated antifreeze peptides. In: 6th Pan Commonwealth Veterinary Conference of the CVA and 27th Veterinary Association Malaysia Congress 2015 (PCVC6 & 27VAM), 23-27 Mar. 2015, The Royale Chulan Hotel, Kuala Lumpur, Malaysia. (pp. 473-476).
institution Universiti Putra Malaysia
building UPM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Putra Malaysia
content_source UPM Institutional Repository
url_provider http://psasir.upm.edu.my/
language English
description Sub-zero injuries in living tissue as a result of re-crystallization phenomenon is the major obstacle and complicates the application of cryopreserved tissues destined for transplantation. Understanding the freezing and thawing response of tissue against the sub-zero temperature would provide better understanding in the application of the non-toxic preservation technique of living tissues. The aim of this study was to evaluate the potential of antifreeze peptides (AFP) as a cryopreservative agent for living tissue e.g. skin. To determine the effects of cryopreservation on the tissue using AFP, type 1 AFP (peptide 1 m) was used to cryopreserve skin graft in different concentrations: 0.5, 1.0 and 2.0 mg/ml at -10°C or -20°C for 24 hrs. The histological distortions effects of the tissues were measured using a scoring system to determine the extent of freezing damage incurred in cryopreserved skin tissues. It was found that relatively less microscopic tissue damages occurred at -10°C compared to -20°C at higher AFP concentration. It is concluded that skin tissue cryopreservation could maintain comparatively better microscopic tissue integrity at -10°C using 2 mg/ml concentration of AFP type 1 as the cryopreservative agent.
format Conference or Workshop Item
author Khan, Muhammad Shoaib
Abubakar, Adamu Abdul
Abdul Rahman, Mohd Basyaruddin
Abu Bakar @ Zakaria, Md Zuki
Mohamed Mustapha, Noordin
Mohamad Yusof, Loqman
spellingShingle Khan, Muhammad Shoaib
Abubakar, Adamu Abdul
Abdul Rahman, Mohd Basyaruddin
Abu Bakar @ Zakaria, Md Zuki
Mohamed Mustapha, Noordin
Mohamad Yusof, Loqman
Histological assesment of low temperature epidermal tissue damage in skin graft cryopreserved with antarctic yeast isolated antifreeze peptides
author_facet Khan, Muhammad Shoaib
Abubakar, Adamu Abdul
Abdul Rahman, Mohd Basyaruddin
Abu Bakar @ Zakaria, Md Zuki
Mohamed Mustapha, Noordin
Mohamad Yusof, Loqman
author_sort Khan, Muhammad Shoaib
title Histological assesment of low temperature epidermal tissue damage in skin graft cryopreserved with antarctic yeast isolated antifreeze peptides
title_short Histological assesment of low temperature epidermal tissue damage in skin graft cryopreserved with antarctic yeast isolated antifreeze peptides
title_full Histological assesment of low temperature epidermal tissue damage in skin graft cryopreserved with antarctic yeast isolated antifreeze peptides
title_fullStr Histological assesment of low temperature epidermal tissue damage in skin graft cryopreserved with antarctic yeast isolated antifreeze peptides
title_full_unstemmed Histological assesment of low temperature epidermal tissue damage in skin graft cryopreserved with antarctic yeast isolated antifreeze peptides
title_sort histological assesment of low temperature epidermal tissue damage in skin graft cryopreserved with antarctic yeast isolated antifreeze peptides
publishDate 2015
url http://psasir.upm.edu.my/id/eprint/67186/1/6th%20PCVC%20AND%2027th%20VAM-13.pdf
http://psasir.upm.edu.my/id/eprint/67186/
_version_ 1643838829588643840
score 13.211869

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