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Effect of sample treatment methods for PAH4 determination in cocoa

Polycyclic aromatic hydrocarbons (PAHs) are carcinogenic and some have been reported to be present in cocoa. Therefore, PAHs determination in cocoa is very important. In this study, a simple procedures based on maceration extraction, solid phase extraction (SPE), saponification and high-performance...

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Main Authors: Agus, Baizura Aya Putri, Hussain, Norhayati
Format: Conference or Workshop Item
Language:English
Published: 2016
Online Access:http://psasir.upm.edu.my/id/eprint/66186/1/Effect%20of%20sample%20treatment%20methods%20for%20PAH4%20determination%20in%20cocoa.pdf
http://psasir.upm.edu.my/id/eprint/66186/
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spelling my.upm.eprints.661862019-02-12T06:46:39Z http://psasir.upm.edu.my/id/eprint/66186/ Effect of sample treatment methods for PAH4 determination in cocoa Agus, Baizura Aya Putri Hussain, Norhayati Polycyclic aromatic hydrocarbons (PAHs) are carcinogenic and some have been reported to be present in cocoa. Therefore, PAHs determination in cocoa is very important. In this study, a simple procedures based on maceration extraction, solid phase extraction (SPE), saponification and high-performance liquid chromatography with fluorescence detection (HPLC-FLD) were presented for rapid PAH4 (sum of four different PAHs; benzo (a) anthracene, chrysene, benzo (b) fluoranthene, and benzo (a) pyrene) determination in cocoa samples (cocoa bean, cocoa nib and cocoa shell). The effect of using different sample extractions (different type of solvents and extraction time) and purification methods (different SPE treatments) were investigated. The most satisfactory recoveries (59.83 – 116.99% at concentration levels; 1.00, 5.00 and 10.00 µ/kg) and clean extracts were obtained by extracting the cocoa samples (cocoa nib, cocoa shell and whole cocoa bean) with hexane for two hours and purification with SPE using silica cartridge (cyclohexane as elution solvent). In this study, detection limit was in the range of 3.36 – 13.90 ng/kg thus, the method meets the Commission Regulation (EU) No. 836/2011 and may be useful to be applied for assessment of cocoa beans quality. 2016 Conference or Workshop Item PeerReviewed text en http://psasir.upm.edu.my/id/eprint/66186/1/Effect%20of%20sample%20treatment%20methods%20for%20PAH4%20determination%20in%20cocoa.pdf Agus, Baizura Aya Putri and Hussain, Norhayati (2016) Effect of sample treatment methods for PAH4 determination in cocoa. In: Monash Science Symposium (MSS) 2016, 21-23 Nov. 2016, Monash University Malaysia. .
institution Universiti Putra Malaysia
building UPM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Putra Malaysia
content_source UPM Institutional Repository
url_provider http://psasir.upm.edu.my/
language English
description Polycyclic aromatic hydrocarbons (PAHs) are carcinogenic and some have been reported to be present in cocoa. Therefore, PAHs determination in cocoa is very important. In this study, a simple procedures based on maceration extraction, solid phase extraction (SPE), saponification and high-performance liquid chromatography with fluorescence detection (HPLC-FLD) were presented for rapid PAH4 (sum of four different PAHs; benzo (a) anthracene, chrysene, benzo (b) fluoranthene, and benzo (a) pyrene) determination in cocoa samples (cocoa bean, cocoa nib and cocoa shell). The effect of using different sample extractions (different type of solvents and extraction time) and purification methods (different SPE treatments) were investigated. The most satisfactory recoveries (59.83 – 116.99% at concentration levels; 1.00, 5.00 and 10.00 µ/kg) and clean extracts were obtained by extracting the cocoa samples (cocoa nib, cocoa shell and whole cocoa bean) with hexane for two hours and purification with SPE using silica cartridge (cyclohexane as elution solvent). In this study, detection limit was in the range of 3.36 – 13.90 ng/kg thus, the method meets the Commission Regulation (EU) No. 836/2011 and may be useful to be applied for assessment of cocoa beans quality.
format Conference or Workshop Item
author Agus, Baizura Aya Putri
Hussain, Norhayati
spellingShingle Agus, Baizura Aya Putri
Hussain, Norhayati
Effect of sample treatment methods for PAH4 determination in cocoa
author_facet Agus, Baizura Aya Putri
Hussain, Norhayati
author_sort Agus, Baizura Aya Putri
title Effect of sample treatment methods for PAH4 determination in cocoa
title_short Effect of sample treatment methods for PAH4 determination in cocoa
title_full Effect of sample treatment methods for PAH4 determination in cocoa
title_fullStr Effect of sample treatment methods for PAH4 determination in cocoa
title_full_unstemmed Effect of sample treatment methods for PAH4 determination in cocoa
title_sort effect of sample treatment methods for pah4 determination in cocoa
publishDate 2016
url http://psasir.upm.edu.my/id/eprint/66186/1/Effect%20of%20sample%20treatment%20methods%20for%20PAH4%20determination%20in%20cocoa.pdf
http://psasir.upm.edu.my/id/eprint/66186/
_version_ 1643838530446688256
score 13.211869

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