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Flow cytometric analysis of platelets mepacrine-labelled dense granules among individuals with mild bleeding symptoms

Introduction: Mild bleeding symptoms are commonly encountered in the general population & amongst individuals with platelet disorders. One of the possible causes is due to reduced number of dense granules synthesis in platelets and defective release of its contents. This study was aimed to evalu...

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Main Authors: Muhsin, Azlina, Mohd Tohit, Eusni Rahayu, Md Noor, Sabariah, Abdul Karim, Faraizah
Format: Article
Language:English
Published: Faculty of Medicine and Health Sciences, Universiti Putra Malaysia 2018
Online Access:http://psasir.upm.edu.my/id/eprint/66172/1/2018121312071404_MJMHS_December_2018.pdf
http://psasir.upm.edu.my/id/eprint/66172/
http://www.medic.upm.edu.my/upload/dokumen/2018121312071404_MJMHS_December_2018.pdf
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spelling my.upm.eprints.661722019-02-11T03:59:27Z http://psasir.upm.edu.my/id/eprint/66172/ Flow cytometric analysis of platelets mepacrine-labelled dense granules among individuals with mild bleeding symptoms Muhsin, Azlina Mohd Tohit, Eusni Rahayu Md Noor, Sabariah Abdul Karim, Faraizah Introduction: Mild bleeding symptoms are commonly encountered in the general population & amongst individuals with platelet disorders. One of the possible causes is due to reduced number of dense granules synthesis in platelets and defective release of its contents. This study was aimed to evaluate platelets mepacrine-labelled dense granules storage and release using flow cytometry in healthy individuals and those presenting with mild bleeding symptoms. Methods: This study was conducted at the National Blood Centre (NBC) and Faculty of Medicine and Health Sciences, Universiti Putra Malaysia (UPM). Thirty- four individuals were recruited as controls (n=24) and patients (n=10). ADP-activated platelets and mepacrine-labelled dense granules was detected using flow cytometry. Results were expressed as mean fluorescent intensity (MFI) of mepacrine in resting and activated platelets; representing dense granules storage and release, respectively. Statistical analysis was considered significant if p ≤ 0.05. Results: There was a significant difference of mean MFI between resting (1284.3 ± 91.8) and activated platelets (1233.8 ± 107.8) of overall respondents with mean difference of 50.5 (p<0.01). However, there was no significant difference of mean MFI in resting and activated platelets between controls and patients was observed. Conclusion: Results indicated there is no secretion defects in platelet dense granules among patients in comparison with controls. Flow cytometry provides alternative way of dense granule assessment in patients presented with mild bleeding symptoms. Faculty of Medicine and Health Sciences, Universiti Putra Malaysia 2018 Article PeerReviewed text en http://psasir.upm.edu.my/id/eprint/66172/1/2018121312071404_MJMHS_December_2018.pdf Muhsin, Azlina and Mohd Tohit, Eusni Rahayu and Md Noor, Sabariah and Abdul Karim, Faraizah (2018) Flow cytometric analysis of platelets mepacrine-labelled dense granules among individuals with mild bleeding symptoms. Malaysian Journal of Medicine and Health Sciences, 14 (SP3). pp. 31-35. ISSN 1675-8544; ESSN: 2636-9346 http://www.medic.upm.edu.my/upload/dokumen/2018121312071404_MJMHS_December_2018.pdf
institution Universiti Putra Malaysia
building UPM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Putra Malaysia
content_source UPM Institutional Repository
url_provider http://psasir.upm.edu.my/
language English
description Introduction: Mild bleeding symptoms are commonly encountered in the general population & amongst individuals with platelet disorders. One of the possible causes is due to reduced number of dense granules synthesis in platelets and defective release of its contents. This study was aimed to evaluate platelets mepacrine-labelled dense granules storage and release using flow cytometry in healthy individuals and those presenting with mild bleeding symptoms. Methods: This study was conducted at the National Blood Centre (NBC) and Faculty of Medicine and Health Sciences, Universiti Putra Malaysia (UPM). Thirty- four individuals were recruited as controls (n=24) and patients (n=10). ADP-activated platelets and mepacrine-labelled dense granules was detected using flow cytometry. Results were expressed as mean fluorescent intensity (MFI) of mepacrine in resting and activated platelets; representing dense granules storage and release, respectively. Statistical analysis was considered significant if p ≤ 0.05. Results: There was a significant difference of mean MFI between resting (1284.3 ± 91.8) and activated platelets (1233.8 ± 107.8) of overall respondents with mean difference of 50.5 (p<0.01). However, there was no significant difference of mean MFI in resting and activated platelets between controls and patients was observed. Conclusion: Results indicated there is no secretion defects in platelet dense granules among patients in comparison with controls. Flow cytometry provides alternative way of dense granule assessment in patients presented with mild bleeding symptoms.
format Article
author Muhsin, Azlina
Mohd Tohit, Eusni Rahayu
Md Noor, Sabariah
Abdul Karim, Faraizah
spellingShingle Muhsin, Azlina
Mohd Tohit, Eusni Rahayu
Md Noor, Sabariah
Abdul Karim, Faraizah
Flow cytometric analysis of platelets mepacrine-labelled dense granules among individuals with mild bleeding symptoms
author_facet Muhsin, Azlina
Mohd Tohit, Eusni Rahayu
Md Noor, Sabariah
Abdul Karim, Faraizah
author_sort Muhsin, Azlina
title Flow cytometric analysis of platelets mepacrine-labelled dense granules among individuals with mild bleeding symptoms
title_short Flow cytometric analysis of platelets mepacrine-labelled dense granules among individuals with mild bleeding symptoms
title_full Flow cytometric analysis of platelets mepacrine-labelled dense granules among individuals with mild bleeding symptoms
title_fullStr Flow cytometric analysis of platelets mepacrine-labelled dense granules among individuals with mild bleeding symptoms
title_full_unstemmed Flow cytometric analysis of platelets mepacrine-labelled dense granules among individuals with mild bleeding symptoms
title_sort flow cytometric analysis of platelets mepacrine-labelled dense granules among individuals with mild bleeding symptoms
publisher Faculty of Medicine and Health Sciences, Universiti Putra Malaysia
publishDate 2018
url http://psasir.upm.edu.my/id/eprint/66172/1/2018121312071404_MJMHS_December_2018.pdf
http://psasir.upm.edu.my/id/eprint/66172/
http://www.medic.upm.edu.my/upload/dokumen/2018121312071404_MJMHS_December_2018.pdf
_version_ 1643838526238752768
score 13.211869

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