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Observation of adverse effect on level ammonia through expression of CD8 lymphocyte in mice

Introduction: The production of ammonia has been increasing over the past few years. Unfortunately, the production does not follow the safety control of ammonia on workers. Indonesia still adopts chemical standard from other countries. Therefore, it requires an ammonia standard at the highest dose w...

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Main Authors: Tualeka, Abdul Rohim, Jalaludin, Juliana
Format: Article
Language:English
Published: Faculty of Medicine and Health Sciences, Universiti Putra Malaysia 2018
Online Access:http://psasir.upm.edu.my/id/eprint/66159/1/2018120408591110_MJMHS_SP_Nov_2018.pdf
http://psasir.upm.edu.my/id/eprint/66159/
http://www.medic.upm.edu.my/upload/dokumen/2018120408591110_MJMHS_SP_Nov_2018.pdf
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spelling my.upm.eprints.661592019-02-11T03:57:47Z http://psasir.upm.edu.my/id/eprint/66159/ Observation of adverse effect on level ammonia through expression of CD8 lymphocyte in mice Tualeka, Abdul Rohim Jalaludin, Juliana Introduction: The production of ammonia has been increasing over the past few years. Unfortunately, the production does not follow the safety control of ammonia on workers. Indonesia still adopts chemical standard from other countries. Therefore, it requires an ammonia standard at the highest dose without effect or no observed adverse effect level (NOAEL) in the workplace. This research aims to determine standard at the highest dose of without effect through the expression of CD8 cells as well as analysis of histological alteration CD8 lymphocyte between exposed to ammonia group and control. Methods: The study was a laboratory experimental research with a post-test only control group design. The research used Rattus novergicus species as many as 24. NOAEL was determined by middle dose with a location between the smallest and the largest dose. The doses of ammonia were given through inhalation. The histological alteration of CD8 between ammonia in exposed and the control group were analyzed by using the Kruskal Wallis test. Results: NOAEL was found through CD8 located in group 3 with 0.0154 dose mg/kg body weight. There was a differential expression of CD8 lymphocyte cells in the white mice lung between exposed to ammonia group and control (p=0.042). Conclusion: The expression of CD8 lymphocyte cells in the white mice lung exposed to ammonia differs significantly with the number of the expression of CD8 lymphocyte cells in white mice lung at control group. NOAEL was 0.0154 mg/kg body weight of white mice. Faculty of Medicine and Health Sciences, Universiti Putra Malaysia 2018 Article PeerReviewed text en http://psasir.upm.edu.my/id/eprint/66159/1/2018120408591110_MJMHS_SP_Nov_2018.pdf Tualeka, Abdul Rohim and Jalaludin, Juliana (2018) Observation of adverse effect on level ammonia through expression of CD8 lymphocyte in mice. Malaysian Journal of Medicine and Health Sciences, 14 (SP2). pp. 71-77. ISSN 1675-8544; ESSN: 2636-9346 http://www.medic.upm.edu.my/upload/dokumen/2018120408591110_MJMHS_SP_Nov_2018.pdf
institution Universiti Putra Malaysia
building UPM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Putra Malaysia
content_source UPM Institutional Repository
url_provider http://psasir.upm.edu.my/
language English
description Introduction: The production of ammonia has been increasing over the past few years. Unfortunately, the production does not follow the safety control of ammonia on workers. Indonesia still adopts chemical standard from other countries. Therefore, it requires an ammonia standard at the highest dose without effect or no observed adverse effect level (NOAEL) in the workplace. This research aims to determine standard at the highest dose of without effect through the expression of CD8 cells as well as analysis of histological alteration CD8 lymphocyte between exposed to ammonia group and control. Methods: The study was a laboratory experimental research with a post-test only control group design. The research used Rattus novergicus species as many as 24. NOAEL was determined by middle dose with a location between the smallest and the largest dose. The doses of ammonia were given through inhalation. The histological alteration of CD8 between ammonia in exposed and the control group were analyzed by using the Kruskal Wallis test. Results: NOAEL was found through CD8 located in group 3 with 0.0154 dose mg/kg body weight. There was a differential expression of CD8 lymphocyte cells in the white mice lung between exposed to ammonia group and control (p=0.042). Conclusion: The expression of CD8 lymphocyte cells in the white mice lung exposed to ammonia differs significantly with the number of the expression of CD8 lymphocyte cells in white mice lung at control group. NOAEL was 0.0154 mg/kg body weight of white mice.
format Article
author Tualeka, Abdul Rohim
Jalaludin, Juliana
spellingShingle Tualeka, Abdul Rohim
Jalaludin, Juliana
Observation of adverse effect on level ammonia through expression of CD8 lymphocyte in mice
author_facet Tualeka, Abdul Rohim
Jalaludin, Juliana
author_sort Tualeka, Abdul Rohim
title Observation of adverse effect on level ammonia through expression of CD8 lymphocyte in mice
title_short Observation of adverse effect on level ammonia through expression of CD8 lymphocyte in mice
title_full Observation of adverse effect on level ammonia through expression of CD8 lymphocyte in mice
title_fullStr Observation of adverse effect on level ammonia through expression of CD8 lymphocyte in mice
title_full_unstemmed Observation of adverse effect on level ammonia through expression of CD8 lymphocyte in mice
title_sort observation of adverse effect on level ammonia through expression of cd8 lymphocyte in mice
publisher Faculty of Medicine and Health Sciences, Universiti Putra Malaysia
publishDate 2018
url http://psasir.upm.edu.my/id/eprint/66159/1/2018120408591110_MJMHS_SP_Nov_2018.pdf
http://psasir.upm.edu.my/id/eprint/66159/
http://www.medic.upm.edu.my/upload/dokumen/2018120408591110_MJMHS_SP_Nov_2018.pdf
_version_ 1643838521092341760
score 13.211869

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