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Antimicrobial activity of Boesenbergia rotunda (L.) Mansf. A. Extract against multi-antibiotics resistant foodborne pathogens

Evolution of resistance in foodborne pathogens to the currently available antibiotics in the market demonstrate the importance of identifying novel antimicrobial agent. Therefore, in this study, Boesenbergia rotunda (fingerroot) from Zingiberaceae family was chosen to be further assessed as antimicr...

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Main Author: Mohd Zainin, Nurul Syazwani
Format: Thesis
Language:English
Published: 2015
Online Access:http://psasir.upm.edu.my/id/eprint/65387/1/FSTM%202015%2017IR.pdf
http://psasir.upm.edu.my/id/eprint/65387/
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spelling my.upm.eprints.653872018-09-14T02:31:45Z http://psasir.upm.edu.my/id/eprint/65387/ Antimicrobial activity of Boesenbergia rotunda (L.) Mansf. A. Extract against multi-antibiotics resistant foodborne pathogens Mohd Zainin, Nurul Syazwani Evolution of resistance in foodborne pathogens to the currently available antibiotics in the market demonstrate the importance of identifying novel antimicrobial agent. Therefore, in this study, Boesenbergia rotunda (fingerroot) from Zingiberaceae family was chosen to be further assessed as antimicrobial agent against eight species of reference foodborne pathogens including its multi-antibiotics resistant isolates, namely Escherichia coli, Bacillus cereus, Klebsiella pneumoniae, Listeria monocytogenes, Salmonella spp., Staphylococcus aureus, Vibrio parahaemolyticus and Candida albicans. B. rotunda methanolic extract showed antimicrobial activity against the microorganisms tested. The ranges of MIC and MBC obtained showed that B. rotunda extract had strong antimicrobial activity against V. parahaemolyticus, B. cereus and S. aureus KCCM 12255 with 9.77 – 312.5 µg/ml and 9.77 – 625 µg/ml, respectively. Meanwhile, the B. rotunda extract was least active against L. monocytogenes, Salmonella spp. and C. albicans with MIC values range were 1250 – 5000 µg/ml and MBC/MFC values range were 2500 - >5000 µg/ml. E. coli and K. pneumoniae showed wide range of inhibition and bactericidal concentrations which were 19.53 – 2500 µg/ml and 39.06 – 5000 µg/ml, respectively. The time-kill curves demonstrated that B. rotunda extract can kill the microorganisms tested with more than 3 log10 (99.99%) within 0.5 to 4 hours of incubation with bactericidal endpoints for B. cereus, V. parahaemolyticus, E. coli ATCC 25922 and S. aureus KCCM 12255 were from 78.13 – 1250 µg/ml (2 MIC and 4 MIC), whereas the bactericidal/fungicidal endpoints for isolated E. coli O157:H7, K. pneumoniae, L. monocytogenes, Salmonella spp. including C. albicans ATCC 14503 were from 2500 – 5000 µg/ml (2 MIC, 4 MIC, and 8 MIC). Generally, the antimicrobial activities of B. rotunda extract were not significantly affected by pH (3, 7, and 11) and temperatures (30˚C, 50˚C, 80˚C and 121˚C). Natural microflora in fruit and vegetable samples also can be reduced at least by 2 log10 CFU/g after treatment with 0.05% (0.5 mg/ml), 0.5% (5 mg/ml) and 5% (50 mg/ml) of B. rotunda extract. Among these treatment solutions, the relative best combination between antimicrobial ability and sensory acceptability can be achieved with 0.05% (0.5 mg/ml) B. rotunda extract, where it showed a significant bacterial population reduction as well as high mean score for the tested sensory attributes. The B. rotunda extract was found not to be toxic with LC50 more than 1.0 mg/ml. GC-MS analysis showed that the antimicrobial properties of the extract possibly attributed by major bioactive compounds, namely camphor, geraniol, methyl cinnamate, pinocembrin and pinostrobin chalcone. It can be concluded that B. rotunda extract has a potential as antimicrobial agent against multi-antibiotics resistant foodborne pathogens which deserves further investigation for development of natural food sanitizer. 2015-05 Thesis NonPeerReviewed text en http://psasir.upm.edu.my/id/eprint/65387/1/FSTM%202015%2017IR.pdf Mohd Zainin, Nurul Syazwani (2015) Antimicrobial activity of Boesenbergia rotunda (L.) Mansf. A. Extract against multi-antibiotics resistant foodborne pathogens. Masters thesis, Universiti Putra Malaysia.
institution Universiti Putra Malaysia
building UPM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Putra Malaysia
content_source UPM Institutional Repository
url_provider http://psasir.upm.edu.my/
language English
description Evolution of resistance in foodborne pathogens to the currently available antibiotics in the market demonstrate the importance of identifying novel antimicrobial agent. Therefore, in this study, Boesenbergia rotunda (fingerroot) from Zingiberaceae family was chosen to be further assessed as antimicrobial agent against eight species of reference foodborne pathogens including its multi-antibiotics resistant isolates, namely Escherichia coli, Bacillus cereus, Klebsiella pneumoniae, Listeria monocytogenes, Salmonella spp., Staphylococcus aureus, Vibrio parahaemolyticus and Candida albicans. B. rotunda methanolic extract showed antimicrobial activity against the microorganisms tested. The ranges of MIC and MBC obtained showed that B. rotunda extract had strong antimicrobial activity against V. parahaemolyticus, B. cereus and S. aureus KCCM 12255 with 9.77 – 312.5 µg/ml and 9.77 – 625 µg/ml, respectively. Meanwhile, the B. rotunda extract was least active against L. monocytogenes, Salmonella spp. and C. albicans with MIC values range were 1250 – 5000 µg/ml and MBC/MFC values range were 2500 - >5000 µg/ml. E. coli and K. pneumoniae showed wide range of inhibition and bactericidal concentrations which were 19.53 – 2500 µg/ml and 39.06 – 5000 µg/ml, respectively. The time-kill curves demonstrated that B. rotunda extract can kill the microorganisms tested with more than 3 log10 (99.99%) within 0.5 to 4 hours of incubation with bactericidal endpoints for B. cereus, V. parahaemolyticus, E. coli ATCC 25922 and S. aureus KCCM 12255 were from 78.13 – 1250 µg/ml (2 MIC and 4 MIC), whereas the bactericidal/fungicidal endpoints for isolated E. coli O157:H7, K. pneumoniae, L. monocytogenes, Salmonella spp. including C. albicans ATCC 14503 were from 2500 – 5000 µg/ml (2 MIC, 4 MIC, and 8 MIC). Generally, the antimicrobial activities of B. rotunda extract were not significantly affected by pH (3, 7, and 11) and temperatures (30˚C, 50˚C, 80˚C and 121˚C). Natural microflora in fruit and vegetable samples also can be reduced at least by 2 log10 CFU/g after treatment with 0.05% (0.5 mg/ml), 0.5% (5 mg/ml) and 5% (50 mg/ml) of B. rotunda extract. Among these treatment solutions, the relative best combination between antimicrobial ability and sensory acceptability can be achieved with 0.05% (0.5 mg/ml) B. rotunda extract, where it showed a significant bacterial population reduction as well as high mean score for the tested sensory attributes. The B. rotunda extract was found not to be toxic with LC50 more than 1.0 mg/ml. GC-MS analysis showed that the antimicrobial properties of the extract possibly attributed by major bioactive compounds, namely camphor, geraniol, methyl cinnamate, pinocembrin and pinostrobin chalcone. It can be concluded that B. rotunda extract has a potential as antimicrobial agent against multi-antibiotics resistant foodborne pathogens which deserves further investigation for development of natural food sanitizer.
format Thesis
author Mohd Zainin, Nurul Syazwani
spellingShingle Mohd Zainin, Nurul Syazwani
Antimicrobial activity of Boesenbergia rotunda (L.) Mansf. A. Extract against multi-antibiotics resistant foodborne pathogens
author_facet Mohd Zainin, Nurul Syazwani
author_sort Mohd Zainin, Nurul Syazwani
title Antimicrobial activity of Boesenbergia rotunda (L.) Mansf. A. Extract against multi-antibiotics resistant foodborne pathogens
title_short Antimicrobial activity of Boesenbergia rotunda (L.) Mansf. A. Extract against multi-antibiotics resistant foodborne pathogens
title_full Antimicrobial activity of Boesenbergia rotunda (L.) Mansf. A. Extract against multi-antibiotics resistant foodborne pathogens
title_fullStr Antimicrobial activity of Boesenbergia rotunda (L.) Mansf. A. Extract against multi-antibiotics resistant foodborne pathogens
title_full_unstemmed Antimicrobial activity of Boesenbergia rotunda (L.) Mansf. A. Extract against multi-antibiotics resistant foodborne pathogens
title_sort antimicrobial activity of boesenbergia rotunda (l.) mansf. a. extract against multi-antibiotics resistant foodborne pathogens
publishDate 2015
url http://psasir.upm.edu.my/id/eprint/65387/1/FSTM%202015%2017IR.pdf
http://psasir.upm.edu.my/id/eprint/65387/
_version_ 1643838299557593088
score 13.211869

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