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Vascular pathology associated with Leptospira icterohaemorrhagiae serovar Lai strain Langkawi infection in guinea pigs (Cavia porcellus)

Fifteen (15) guinea pigs were experimentally infected with Leptospira icterohemorrhagiae serovar Lai strain Langkawi, a new strain that was isolated from a human leptospirosis patient. Hematoxylin and Eosin ((H&E) staining showed haemorrhages, congestion and oedema in all internal organs examine...

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Main Authors: Tyagita, G. H., Bahaman, Abdul Rani, Sabri, Jasni, Hussein, Fuzina Nor, Tengku Ibrahim, Tengku Azmi
Format: Article
Language:English
Published: Veterinary Association Malaysia 2018
Online Access:http://psasir.upm.edu.my/id/eprint/65329/1/JVM-2018-Issue-1_Prof-Rani.pdf
http://psasir.upm.edu.my/id/eprint/65329/
http://jvm.vam.org.my/wp-content/uploads/2018/07/JVM-2018-Issue-1_Prof-Rani.pdf
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spelling my.upm.eprints.653292018-10-05T07:39:19Z http://psasir.upm.edu.my/id/eprint/65329/ Vascular pathology associated with Leptospira icterohaemorrhagiae serovar Lai strain Langkawi infection in guinea pigs (Cavia porcellus) Tyagita, G. H. Bahaman, Abdul Rani Sabri, Jasni Hussein, Fuzina Nor Tengku Ibrahim, Tengku Azmi Fifteen (15) guinea pigs were experimentally infected with Leptospira icterohemorrhagiae serovar Lai strain Langkawi, a new strain that was isolated from a human leptospirosis patient. Hematoxylin and Eosin ((H&E) staining showed haemorrhages, congestion and oedema in all internal organs examined (lungs, liver, spleen and kidneys) with inflammatory cell infiltration characterized by neutrophils, lymphocytes and macrophages. Hydropic degeneration and cell necrosis were also common in our findings. Leptospires were detected starting Day 2 p.i by silver staining and Transmission Electron Microscopy (TEM). Rise in antibody titres started on Day 5 p.i and leptospiral DNA was detected beginning Day 3 in the kidneys and Day 5 in the liver by Polymerase Chain Reaction (PCR) assay. The findings illustrated the pathogenesis of leptospirosis in guinea pigs which disclosed them as a suitable animal model for demonstration of clinical symptoms of leptospirosis and pathological changes after being infected with Leptospira icterohaemorrhagiae serovar Lai strain Langkawi, particularly pulmonary haemorrhages, a leading cause of mortality in human leptospirosis. Veterinary Association Malaysia 2018 Article PeerReviewed text en http://psasir.upm.edu.my/id/eprint/65329/1/JVM-2018-Issue-1_Prof-Rani.pdf Tyagita, G. H. and Bahaman, Abdul Rani and Sabri, Jasni and Hussein, Fuzina Nor and Tengku Ibrahim, Tengku Azmi (2018) Vascular pathology associated with Leptospira icterohaemorrhagiae serovar Lai strain Langkawi infection in guinea pigs (Cavia porcellus). Jurnal Veterinar Malaysia, 30 (1). pp. 7-14. ISSN 9128-2506 http://jvm.vam.org.my/wp-content/uploads/2018/07/JVM-2018-Issue-1_Prof-Rani.pdf
institution Universiti Putra Malaysia
building UPM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Putra Malaysia
content_source UPM Institutional Repository
url_provider http://psasir.upm.edu.my/
language English
description Fifteen (15) guinea pigs were experimentally infected with Leptospira icterohemorrhagiae serovar Lai strain Langkawi, a new strain that was isolated from a human leptospirosis patient. Hematoxylin and Eosin ((H&E) staining showed haemorrhages, congestion and oedema in all internal organs examined (lungs, liver, spleen and kidneys) with inflammatory cell infiltration characterized by neutrophils, lymphocytes and macrophages. Hydropic degeneration and cell necrosis were also common in our findings. Leptospires were detected starting Day 2 p.i by silver staining and Transmission Electron Microscopy (TEM). Rise in antibody titres started on Day 5 p.i and leptospiral DNA was detected beginning Day 3 in the kidneys and Day 5 in the liver by Polymerase Chain Reaction (PCR) assay. The findings illustrated the pathogenesis of leptospirosis in guinea pigs which disclosed them as a suitable animal model for demonstration of clinical symptoms of leptospirosis and pathological changes after being infected with Leptospira icterohaemorrhagiae serovar Lai strain Langkawi, particularly pulmonary haemorrhages, a leading cause of mortality in human leptospirosis.
format Article
author Tyagita, G. H.
Bahaman, Abdul Rani
Sabri, Jasni
Hussein, Fuzina Nor
Tengku Ibrahim, Tengku Azmi
spellingShingle Tyagita, G. H.
Bahaman, Abdul Rani
Sabri, Jasni
Hussein, Fuzina Nor
Tengku Ibrahim, Tengku Azmi
Vascular pathology associated with Leptospira icterohaemorrhagiae serovar Lai strain Langkawi infection in guinea pigs (Cavia porcellus)
author_facet Tyagita, G. H.
Bahaman, Abdul Rani
Sabri, Jasni
Hussein, Fuzina Nor
Tengku Ibrahim, Tengku Azmi
author_sort Tyagita, G. H.
title Vascular pathology associated with Leptospira icterohaemorrhagiae serovar Lai strain Langkawi infection in guinea pigs (Cavia porcellus)
title_short Vascular pathology associated with Leptospira icterohaemorrhagiae serovar Lai strain Langkawi infection in guinea pigs (Cavia porcellus)
title_full Vascular pathology associated with Leptospira icterohaemorrhagiae serovar Lai strain Langkawi infection in guinea pigs (Cavia porcellus)
title_fullStr Vascular pathology associated with Leptospira icterohaemorrhagiae serovar Lai strain Langkawi infection in guinea pigs (Cavia porcellus)
title_full_unstemmed Vascular pathology associated with Leptospira icterohaemorrhagiae serovar Lai strain Langkawi infection in guinea pigs (Cavia porcellus)
title_sort vascular pathology associated with leptospira icterohaemorrhagiae serovar lai strain langkawi infection in guinea pigs (cavia porcellus)
publisher Veterinary Association Malaysia
publishDate 2018
url http://psasir.upm.edu.my/id/eprint/65329/1/JVM-2018-Issue-1_Prof-Rani.pdf
http://psasir.upm.edu.my/id/eprint/65329/
http://jvm.vam.org.my/wp-content/uploads/2018/07/JVM-2018-Issue-1_Prof-Rani.pdf
_version_ 1643838282949197824
score 13.211869

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