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Genetic relationships among Malaysian banana cultivars revealed by chloroplast SSR

Bananas and plantains have been highly esteemed and widely used by man since ancient times. Fiber contents in bananas and plantains act as an aid for digestion. There are also reports that ripe bananas are used in the treatment of asthma and bronchitis. In breeding programs, the breeds valued charac...

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Main Authors: Tee, Meng Han, Daud, Siti Khalijah
Format: Conference or Workshop Item
Language:English
Published: 2009
Online Access:http://psasir.upm.edu.my/id/eprint/65126/1/PGM-1-6.pdf
http://psasir.upm.edu.my/id/eprint/65126/
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spelling my.upm.eprints.651262018-09-04T04:04:11Z http://psasir.upm.edu.my/id/eprint/65126/ Genetic relationships among Malaysian banana cultivars revealed by chloroplast SSR Tee, Meng Han Daud, Siti Khalijah Bananas and plantains have been highly esteemed and widely used by man since ancient times. Fiber contents in bananas and plantains act as an aid for digestion. There are also reports that ripe bananas are used in the treatment of asthma and bronchitis. In breeding programs, the breeds valued characteristics should be maintained during its further improvement. A Chloroplast Simple Sequence Repeat (SSR) analysis was carried out to determine variation and relationships among local banana cultivars. Eight banana cultivars were used in this study, namely Mas (AA), Berangan (AAA), Raja (AAB), Rastali (AAB), Awak (ABB), Nipah (BBB), Kapas (AA) and Nangka (AAB). Twenty five individuals of each cultivar were collected from Perak, Selangor, Johor, Pahang Kedah, Kelantan and Negeri Sembilan. Sixteen Microsatellite primer pairs were selected to amplify the chloroplast DNA. The highest genetic distance was found between Pisang Nipah and Pisang Raja (0.0654) whilst the lowest was between Pisang Berangan and Pisang Awak (0.0036). The dendrogram showed that Pisang Raja and Pisang Rastali were grouped together and they are of similar genotypes. However, Pisang Awak and Pisang Berangan have the lowest genetic distance and grouped together although their genotypes are different. The results of this study suggest that the clustering of these bananas does not necessarily follow their ploidy levels. 2009 Conference or Workshop Item PeerReviewed text en http://psasir.upm.edu.my/id/eprint/65126/1/PGM-1-6.pdf Tee, Meng Han and Daud, Siti Khalijah (2009) Genetic relationships among Malaysian banana cultivars revealed by chloroplast SSR. In: 8th Malaysia Congress on Genetics, 4-6 Aug. 2009, Genting Highlands, Malaysia. (pp. 87-90).
institution Universiti Putra Malaysia
building UPM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Putra Malaysia
content_source UPM Institutional Repository
url_provider http://psasir.upm.edu.my/
language English
description Bananas and plantains have been highly esteemed and widely used by man since ancient times. Fiber contents in bananas and plantains act as an aid for digestion. There are also reports that ripe bananas are used in the treatment of asthma and bronchitis. In breeding programs, the breeds valued characteristics should be maintained during its further improvement. A Chloroplast Simple Sequence Repeat (SSR) analysis was carried out to determine variation and relationships among local banana cultivars. Eight banana cultivars were used in this study, namely Mas (AA), Berangan (AAA), Raja (AAB), Rastali (AAB), Awak (ABB), Nipah (BBB), Kapas (AA) and Nangka (AAB). Twenty five individuals of each cultivar were collected from Perak, Selangor, Johor, Pahang Kedah, Kelantan and Negeri Sembilan. Sixteen Microsatellite primer pairs were selected to amplify the chloroplast DNA. The highest genetic distance was found between Pisang Nipah and Pisang Raja (0.0654) whilst the lowest was between Pisang Berangan and Pisang Awak (0.0036). The dendrogram showed that Pisang Raja and Pisang Rastali were grouped together and they are of similar genotypes. However, Pisang Awak and Pisang Berangan have the lowest genetic distance and grouped together although their genotypes are different. The results of this study suggest that the clustering of these bananas does not necessarily follow their ploidy levels.
format Conference or Workshop Item
author Tee, Meng Han
Daud, Siti Khalijah
spellingShingle Tee, Meng Han
Daud, Siti Khalijah
Genetic relationships among Malaysian banana cultivars revealed by chloroplast SSR
author_facet Tee, Meng Han
Daud, Siti Khalijah
author_sort Tee, Meng Han
title Genetic relationships among Malaysian banana cultivars revealed by chloroplast SSR
title_short Genetic relationships among Malaysian banana cultivars revealed by chloroplast SSR
title_full Genetic relationships among Malaysian banana cultivars revealed by chloroplast SSR
title_fullStr Genetic relationships among Malaysian banana cultivars revealed by chloroplast SSR
title_full_unstemmed Genetic relationships among Malaysian banana cultivars revealed by chloroplast SSR
title_sort genetic relationships among malaysian banana cultivars revealed by chloroplast ssr
publishDate 2009
url http://psasir.upm.edu.my/id/eprint/65126/1/PGM-1-6.pdf
http://psasir.upm.edu.my/id/eprint/65126/
_version_ 1643838227391447040
score 13.211869

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