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Performance of real-time polymerase chain reaction in the detection of infectious bursal disease virus

The sensitivity of SYBR Green 1 based real-time PCR to detect infectious bursal disease virus (IBDV) was compared with an ELISA nested RT-PCR and conventional gel electrophoresis methods. The real-time PCR was at least 10 fold more sensitive than the ELISA nested RT-PCR, while the ELISA nested PCR w...

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Main Authors: Hamzah, Hairul Aini, Omar, Abdul Rahman, Bejo, Mohd Hair, Ideris, Aini
Format: Conference or Workshop Item
Language:English
Published: Universiti Putra Malaysia Press 2004
Online Access:http://psasir.upm.edu.my/id/eprint/65106/1/71-15.pdf
http://psasir.upm.edu.my/id/eprint/65106/
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spelling my.upm.eprints.651062018-09-04T04:01:51Z http://psasir.upm.edu.my/id/eprint/65106/ Performance of real-time polymerase chain reaction in the detection of infectious bursal disease virus Hamzah, Hairul Aini Omar, Abdul Rahman Bejo, Mohd Hair Ideris, Aini The sensitivity of SYBR Green 1 based real-time PCR to detect infectious bursal disease virus (IBDV) was compared with an ELISA nested RT-PCR and conventional gel electrophoresis methods. The real-time PCR was at least 10 fold more sensitive than the ELISA nested RT-PCR, while the ELISA nested PCR was at least 10 fold more sensitive than the conventional gel electrophoresis. Thus, real-time PCR using SYBR Green 1 offers a sensitive, rapid and convenient method for screening large number of IBDV. Universiti Putra Malaysia Press 2004 Conference or Workshop Item PeerReviewed text en http://psasir.upm.edu.my/id/eprint/65106/1/71-15.pdf Hamzah, Hairul Aini and Omar, Abdul Rahman and Bejo, Mohd Hair and Ideris, Aini (2004) Performance of real-time polymerase chain reaction in the detection of infectious bursal disease virus. In: 11th International Conference of the Association of Institutions for Tropical Veterinary Medicine and 16th Veterinary Association Malaysia Congress, 23-27 Aug. 2004, Sunway Pyramid Convention Centre, Petaling Jaya, Malaysia. (pp. 226-227).
institution Universiti Putra Malaysia
building UPM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Putra Malaysia
content_source UPM Institutional Repository
url_provider http://psasir.upm.edu.my/
language English
description The sensitivity of SYBR Green 1 based real-time PCR to detect infectious bursal disease virus (IBDV) was compared with an ELISA nested RT-PCR and conventional gel electrophoresis methods. The real-time PCR was at least 10 fold more sensitive than the ELISA nested RT-PCR, while the ELISA nested PCR was at least 10 fold more sensitive than the conventional gel electrophoresis. Thus, real-time PCR using SYBR Green 1 offers a sensitive, rapid and convenient method for screening large number of IBDV.
format Conference or Workshop Item
author Hamzah, Hairul Aini
Omar, Abdul Rahman
Bejo, Mohd Hair
Ideris, Aini
spellingShingle Hamzah, Hairul Aini
Omar, Abdul Rahman
Bejo, Mohd Hair
Ideris, Aini
Performance of real-time polymerase chain reaction in the detection of infectious bursal disease virus
author_facet Hamzah, Hairul Aini
Omar, Abdul Rahman
Bejo, Mohd Hair
Ideris, Aini
author_sort Hamzah, Hairul Aini
title Performance of real-time polymerase chain reaction in the detection of infectious bursal disease virus
title_short Performance of real-time polymerase chain reaction in the detection of infectious bursal disease virus
title_full Performance of real-time polymerase chain reaction in the detection of infectious bursal disease virus
title_fullStr Performance of real-time polymerase chain reaction in the detection of infectious bursal disease virus
title_full_unstemmed Performance of real-time polymerase chain reaction in the detection of infectious bursal disease virus
title_sort performance of real-time polymerase chain reaction in the detection of infectious bursal disease virus
publisher Universiti Putra Malaysia Press
publishDate 2004
url http://psasir.upm.edu.my/id/eprint/65106/1/71-15.pdf
http://psasir.upm.edu.my/id/eprint/65106/
_version_ 1643838221409320960
score 13.211869

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