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Bursal transcriptome of different inbred chicken lines following infection with very virulent infectious bursal disease virus

Infectious bursal disease virus (IBDV) targeting IgM-bearing B cells in the bursa of fabricius cause an immunosuppressive effect in young chickens. Infection with IBDV evoked necessary immune response such as infiltration of T cell and macrophages into the bursa which may limit viral replication or...

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Main Authors: Mohd Isa, Noor Farhanah, Bejo, Mohd Hair, Mat Isa, Nurulfiza, Ideris, Aini, Nair, Venugopal, Omar, Abdul Rahman
Format: Conference or Workshop Item
Language:English
Published: Faculty of Veterinary Medicine, Universiti Putra Malaysia 2015
Online Access:http://psasir.upm.edu.my/id/eprint/65017/1/PA-18.pdf
http://psasir.upm.edu.my/id/eprint/65017/
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spelling my.upm.eprints.650172018-09-03T04:41:44Z http://psasir.upm.edu.my/id/eprint/65017/ Bursal transcriptome of different inbred chicken lines following infection with very virulent infectious bursal disease virus Mohd Isa, Noor Farhanah Bejo, Mohd Hair Mat Isa, Nurulfiza Ideris, Aini Nair, Venugopal Omar, Abdul Rahman Infectious bursal disease virus (IBDV) targeting IgM-bearing B cells in the bursa of fabricius cause an immunosuppressive effect in young chickens. Infection with IBDV evoked necessary immune response such as infiltration of T cell and macrophages into the bursa which may limit viral replication or promote bursal tissue damage through cytokine secretion. However, mechanisms of very virulent (vv) IBDV infection in chickens of different genetic backgrounds are poorly characterized. Therefore, this study aims to profile transcriptome expression of bursal tissues of inbred chickens with varying genetic background following infection with vvIBDV using RNA-seq Illumina platform in an effort to have a better understanding in host-pathogen interaction. From six infected chicken lines, four has been determined to be highly susceptible while the remaining two is less susceptible based on viral load quantification. Bursal transcriptome profiling result shows that more than 4,500genes has been identified to be significantly expressed with expression value was based on cut off value; log2 fold-change < -2 or log2 fold-change > 2and q-value < 0.05. Genes that were up-regulated after IBDV infection are those involve in immune regulatory network such as pro-inflammatory cytokines and chemokines, JNK-p38 MAPK signalling genes, and stress-response genes. On the other hand, genes that were down-regulated are those involve in DNA replication, and transcription and translation process. This study has enable us to resolve susceptibility level of various chicken lines and evaluation of bursal transcriptome profile allow us identify possible gene involve in host susceptibility. Faculty of Veterinary Medicine, Universiti Putra Malaysia 2015 Conference or Workshop Item PeerReviewed text en http://psasir.upm.edu.my/id/eprint/65017/1/PA-18.pdf Mohd Isa, Noor Farhanah and Bejo, Mohd Hair and Mat Isa, Nurulfiza and Ideris, Aini and Nair, Venugopal and Omar, Abdul Rahman (2015) Bursal transcriptome of different inbred chicken lines following infection with very virulent infectious bursal disease virus. In: World Veterinary Poultry Association (Malaysia Branch) and World's Poultry Science Association (Malaysia Branch) Scientific Conference 2015, 21-22 Sept. 2015, Kuala Lumpur Convention Centre, Kuala Lumpur, Malaysia. (pp. 122-124).
institution Universiti Putra Malaysia
building UPM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Putra Malaysia
content_source UPM Institutional Repository
url_provider http://psasir.upm.edu.my/
language English
description Infectious bursal disease virus (IBDV) targeting IgM-bearing B cells in the bursa of fabricius cause an immunosuppressive effect in young chickens. Infection with IBDV evoked necessary immune response such as infiltration of T cell and macrophages into the bursa which may limit viral replication or promote bursal tissue damage through cytokine secretion. However, mechanisms of very virulent (vv) IBDV infection in chickens of different genetic backgrounds are poorly characterized. Therefore, this study aims to profile transcriptome expression of bursal tissues of inbred chickens with varying genetic background following infection with vvIBDV using RNA-seq Illumina platform in an effort to have a better understanding in host-pathogen interaction. From six infected chicken lines, four has been determined to be highly susceptible while the remaining two is less susceptible based on viral load quantification. Bursal transcriptome profiling result shows that more than 4,500genes has been identified to be significantly expressed with expression value was based on cut off value; log2 fold-change < -2 or log2 fold-change > 2and q-value < 0.05. Genes that were up-regulated after IBDV infection are those involve in immune regulatory network such as pro-inflammatory cytokines and chemokines, JNK-p38 MAPK signalling genes, and stress-response genes. On the other hand, genes that were down-regulated are those involve in DNA replication, and transcription and translation process. This study has enable us to resolve susceptibility level of various chicken lines and evaluation of bursal transcriptome profile allow us identify possible gene involve in host susceptibility.
format Conference or Workshop Item
author Mohd Isa, Noor Farhanah
Bejo, Mohd Hair
Mat Isa, Nurulfiza
Ideris, Aini
Nair, Venugopal
Omar, Abdul Rahman
spellingShingle Mohd Isa, Noor Farhanah
Bejo, Mohd Hair
Mat Isa, Nurulfiza
Ideris, Aini
Nair, Venugopal
Omar, Abdul Rahman
Bursal transcriptome of different inbred chicken lines following infection with very virulent infectious bursal disease virus
author_facet Mohd Isa, Noor Farhanah
Bejo, Mohd Hair
Mat Isa, Nurulfiza
Ideris, Aini
Nair, Venugopal
Omar, Abdul Rahman
author_sort Mohd Isa, Noor Farhanah
title Bursal transcriptome of different inbred chicken lines following infection with very virulent infectious bursal disease virus
title_short Bursal transcriptome of different inbred chicken lines following infection with very virulent infectious bursal disease virus
title_full Bursal transcriptome of different inbred chicken lines following infection with very virulent infectious bursal disease virus
title_fullStr Bursal transcriptome of different inbred chicken lines following infection with very virulent infectious bursal disease virus
title_full_unstemmed Bursal transcriptome of different inbred chicken lines following infection with very virulent infectious bursal disease virus
title_sort bursal transcriptome of different inbred chicken lines following infection with very virulent infectious bursal disease virus
publisher Faculty of Veterinary Medicine, Universiti Putra Malaysia
publishDate 2015
url http://psasir.upm.edu.my/id/eprint/65017/1/PA-18.pdf
http://psasir.upm.edu.my/id/eprint/65017/
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score 13.211869

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