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Simultaneous separation of killed Pasteurella multocida cells and removal of formalin using tangential flow membrane filtration for production of toxoid vaccines

Formalin is used to inactivate bacterial products for the production of vaccines that use and inactive bacterial toxin to produce immunity, which is commonly known as toxoid vaccines. Formalin is highly toxic to human and animals, regardless of method intake. The commercial production of killed bact...

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Main Authors: Kapri, Mohammad Rizal, Lajis, Ahmad Firdaus, Mohamad, Mazlan, Halim, Murni, Mohd Yusoff, Md Sabri, Ariff, Arbakariya
Format: Conference or Workshop Item
Language:English
Published: Knowledge Transfer Programme – Ministry of Higher Education, Malaysia 2015
Online Access:http://psasir.upm.edu.my/id/eprint/64963/1/T15-4.pdf
http://psasir.upm.edu.my/id/eprint/64963/
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spelling my.upm.eprints.649632018-09-03T04:09:56Z http://psasir.upm.edu.my/id/eprint/64963/ Simultaneous separation of killed Pasteurella multocida cells and removal of formalin using tangential flow membrane filtration for production of toxoid vaccines Kapri, Mohammad Rizal Lajis, Ahmad Firdaus Mohamad, Mazlan Halim, Murni Mohd Yusoff, Md Sabri Ariff, Arbakariya Formalin is used to inactivate bacterial products for the production of vaccines that use and inactive bacterial toxin to produce immunity, which is commonly known as toxoid vaccines. Formalin is highly toxic to human and animals, regardless of method intake. The commercial production of killed bacterial vaccines based on Pasteurella multocida for veterinary use is mainly depends on the effective separation of the killed bacterial cell and separation of formalin from large volumes of fermentation broth. Batch centrifugation followed by washing with buffers is applicable for laboratory scale but not for large scale industrial process. In this project, tangential flow filtration (TFF) or cross flow membrane filtration system was used as rapid drive in the separation of killed bacterial cells of P. multocida and simultaneously to remove formalin from the cell suspension. The fermentation broth containing P. multocida was prepared using stirred tank bioreactor and the bacterium was killed with 3% (V/V) formalin. The separation of killed cells and the removal of formalin were simultaneously performed using TFF with microporous membrane (0.22 and 0.45 μm) operated continuously for several cycles with various modes. The operational variable of the TFF system such as average trans-membrane pressure, cross flow rate and flux were also investigated. Under the best conditions, formalin in concentrated cell suspension was successfully reduced to lower than 0.03% (v/v). The permitted formalin concentration in vaccine product, as suggested by British Pharmacopeia, shall be not exceeded 0.5 g/L. This indicates that TFF system is promising to produce industrial killed bacterial vaccines that comply with good manufacturing practice (GMP) guidelines. Knowledge Transfer Programme – Ministry of Higher Education, Malaysia 2015 Conference or Workshop Item PeerReviewed text en http://psasir.upm.edu.my/id/eprint/64963/1/T15-4.pdf Kapri, Mohammad Rizal and Lajis, Ahmad Firdaus and Mohamad, Mazlan and Halim, Murni and Mohd Yusoff, Md Sabri and Ariff, Arbakariya (2015) Simultaneous separation of killed Pasteurella multocida cells and removal of formalin using tangential flow membrane filtration for production of toxoid vaccines. In: International Conference on Knowledge Transfer (ICKT'15), 1-3 Dec. 2015, Putrajaya, Malaysia. (pp. 48-53).
institution Universiti Putra Malaysia
building UPM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Putra Malaysia
content_source UPM Institutional Repository
url_provider http://psasir.upm.edu.my/
language English
description Formalin is used to inactivate bacterial products for the production of vaccines that use and inactive bacterial toxin to produce immunity, which is commonly known as toxoid vaccines. Formalin is highly toxic to human and animals, regardless of method intake. The commercial production of killed bacterial vaccines based on Pasteurella multocida for veterinary use is mainly depends on the effective separation of the killed bacterial cell and separation of formalin from large volumes of fermentation broth. Batch centrifugation followed by washing with buffers is applicable for laboratory scale but not for large scale industrial process. In this project, tangential flow filtration (TFF) or cross flow membrane filtration system was used as rapid drive in the separation of killed bacterial cells of P. multocida and simultaneously to remove formalin from the cell suspension. The fermentation broth containing P. multocida was prepared using stirred tank bioreactor and the bacterium was killed with 3% (V/V) formalin. The separation of killed cells and the removal of formalin were simultaneously performed using TFF with microporous membrane (0.22 and 0.45 μm) operated continuously for several cycles with various modes. The operational variable of the TFF system such as average trans-membrane pressure, cross flow rate and flux were also investigated. Under the best conditions, formalin in concentrated cell suspension was successfully reduced to lower than 0.03% (v/v). The permitted formalin concentration in vaccine product, as suggested by British Pharmacopeia, shall be not exceeded 0.5 g/L. This indicates that TFF system is promising to produce industrial killed bacterial vaccines that comply with good manufacturing practice (GMP) guidelines.
format Conference or Workshop Item
author Kapri, Mohammad Rizal
Lajis, Ahmad Firdaus
Mohamad, Mazlan
Halim, Murni
Mohd Yusoff, Md Sabri
Ariff, Arbakariya
spellingShingle Kapri, Mohammad Rizal
Lajis, Ahmad Firdaus
Mohamad, Mazlan
Halim, Murni
Mohd Yusoff, Md Sabri
Ariff, Arbakariya
Simultaneous separation of killed Pasteurella multocida cells and removal of formalin using tangential flow membrane filtration for production of toxoid vaccines
author_facet Kapri, Mohammad Rizal
Lajis, Ahmad Firdaus
Mohamad, Mazlan
Halim, Murni
Mohd Yusoff, Md Sabri
Ariff, Arbakariya
author_sort Kapri, Mohammad Rizal
title Simultaneous separation of killed Pasteurella multocida cells and removal of formalin using tangential flow membrane filtration for production of toxoid vaccines
title_short Simultaneous separation of killed Pasteurella multocida cells and removal of formalin using tangential flow membrane filtration for production of toxoid vaccines
title_full Simultaneous separation of killed Pasteurella multocida cells and removal of formalin using tangential flow membrane filtration for production of toxoid vaccines
title_fullStr Simultaneous separation of killed Pasteurella multocida cells and removal of formalin using tangential flow membrane filtration for production of toxoid vaccines
title_full_unstemmed Simultaneous separation of killed Pasteurella multocida cells and removal of formalin using tangential flow membrane filtration for production of toxoid vaccines
title_sort simultaneous separation of killed pasteurella multocida cells and removal of formalin using tangential flow membrane filtration for production of toxoid vaccines
publisher Knowledge Transfer Programme – Ministry of Higher Education, Malaysia
publishDate 2015
url http://psasir.upm.edu.my/id/eprint/64963/1/T15-4.pdf
http://psasir.upm.edu.my/id/eprint/64963/
_version_ 1643838177679507456
score 13.211869

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