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In vitro antibacterial activity of crude medicinal plant extracts against ampicillin+penicillin-resistant Staphylococcus aureus

Staphylococcus aureus is the leading cause for foodborne diseases. Extensive use of antibiotics has led to emergence of antibiotic-resistant S. aureus. Hence, interest on natural plant-based alternative which limits the use of synthetic chemicals is growing. The present work evaluated the antibacter...

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Main Authors: Samsudin, Nik Iskandar Putra, Lee, Hai Yen, Chern, Pei Ern, Ng, Chew Ting, Panneerselvam, Lechumi, Phang, Siew Yin, Tan, Wei Theng, Mahyudin, Nor Ainy
Format: Article
Language:English
Published: Faculty of Food Science and Technology, Universiti Putra Malaysia 2018
Online Access:http://psasir.upm.edu.my/id/eprint/64511/1/%2817%29.pdf
http://psasir.upm.edu.my/id/eprint/64511/
http://www.ifrj.upm.edu.my/25%20(02)%202018/(17).pdf
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spelling my.upm.eprints.645112018-07-06T09:19:28Z http://psasir.upm.edu.my/id/eprint/64511/ In vitro antibacterial activity of crude medicinal plant extracts against ampicillin+penicillin-resistant Staphylococcus aureus Samsudin, Nik Iskandar Putra Lee, Hai Yen Chern, Pei Ern Ng, Chew Ting Panneerselvam, Lechumi Phang, Siew Yin Tan, Wei Theng Mahyudin, Nor Ainy Staphylococcus aureus is the leading cause for foodborne diseases. Extensive use of antibiotics has led to emergence of antibiotic-resistant S. aureus. Hence, interest on natural plant-based alternative which limits the use of synthetic chemicals is growing. The present work evaluated the antibacterial capacity of garlic, aloe vera, galangal, pineapple peel, neem, papaya leaf, lemongrass, peppermint, nutmeg and clove separately extracted with hexane, ethanol and water to a final concentration of 10% w/v against ampicillin+penicillin-resistant isolates of S. aureus in vitro. Streptomycin was used as a drug control against the resistant isolates; BRS023, BRS068 and DRS072. According to the interpretive standards for inhibition zone diameter provided by the Clinical and Laboratory Standards Institute, isolates BRS068 and DRS072 were considered resistant (≤ 12 mm), and isolate BRS023 was considered intermediate (13-14 mm). Against these isolates, all crude plant extracts exhibited varying degrees of inhibition. However, a coherent trend was observed in the inhibition between resistant and intermediate isolates regardless of plants and solvents used. It was also found that extraction solvent types impacted the resulting antibacterial activity. In terms of positive inhibition, the solvents were ranked in the order of hexane (77%) > water (73%) > ethanol (57%). 10% hexane extract of galangal gave the overall highest inhibition zones (17.8 ± 1.4 mm) closely followed by 10% ethanol extract of nutmeg (16.3 ± 1.1 mm). Further phytochemical analyses of the antibacterial compounds from galangal and nutmeg, and their minimum inhibitory concentration (MIC) are needed. Potential applications of plant-based antibacterial compounds as natural, costeffective and less-toxic food preservatives against drug-resistant foodborne pathogens should be explored. Faculty of Food Science and Technology, Universiti Putra Malaysia 2018 Article PeerReviewed text en http://psasir.upm.edu.my/id/eprint/64511/1/%2817%29.pdf Samsudin, Nik Iskandar Putra and Lee, Hai Yen and Chern, Pei Ern and Ng, Chew Ting and Panneerselvam, Lechumi and Phang, Siew Yin and Tan, Wei Theng and Mahyudin, Nor Ainy (2018) In vitro antibacterial activity of crude medicinal plant extracts against ampicillin+penicillin-resistant Staphylococcus aureus. International Food Research Journal, 25 (2). pp. 573-579. ISSN 1985-4668; ESSN: 2231-7546 http://www.ifrj.upm.edu.my/25%20(02)%202018/(17).pdf
institution Universiti Putra Malaysia
building UPM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Putra Malaysia
content_source UPM Institutional Repository
url_provider http://psasir.upm.edu.my/
language English
description Staphylococcus aureus is the leading cause for foodborne diseases. Extensive use of antibiotics has led to emergence of antibiotic-resistant S. aureus. Hence, interest on natural plant-based alternative which limits the use of synthetic chemicals is growing. The present work evaluated the antibacterial capacity of garlic, aloe vera, galangal, pineapple peel, neem, papaya leaf, lemongrass, peppermint, nutmeg and clove separately extracted with hexane, ethanol and water to a final concentration of 10% w/v against ampicillin+penicillin-resistant isolates of S. aureus in vitro. Streptomycin was used as a drug control against the resistant isolates; BRS023, BRS068 and DRS072. According to the interpretive standards for inhibition zone diameter provided by the Clinical and Laboratory Standards Institute, isolates BRS068 and DRS072 were considered resistant (≤ 12 mm), and isolate BRS023 was considered intermediate (13-14 mm). Against these isolates, all crude plant extracts exhibited varying degrees of inhibition. However, a coherent trend was observed in the inhibition between resistant and intermediate isolates regardless of plants and solvents used. It was also found that extraction solvent types impacted the resulting antibacterial activity. In terms of positive inhibition, the solvents were ranked in the order of hexane (77%) > water (73%) > ethanol (57%). 10% hexane extract of galangal gave the overall highest inhibition zones (17.8 ± 1.4 mm) closely followed by 10% ethanol extract of nutmeg (16.3 ± 1.1 mm). Further phytochemical analyses of the antibacterial compounds from galangal and nutmeg, and their minimum inhibitory concentration (MIC) are needed. Potential applications of plant-based antibacterial compounds as natural, costeffective and less-toxic food preservatives against drug-resistant foodborne pathogens should be explored.
format Article
author Samsudin, Nik Iskandar Putra
Lee, Hai Yen
Chern, Pei Ern
Ng, Chew Ting
Panneerselvam, Lechumi
Phang, Siew Yin
Tan, Wei Theng
Mahyudin, Nor Ainy
spellingShingle Samsudin, Nik Iskandar Putra
Lee, Hai Yen
Chern, Pei Ern
Ng, Chew Ting
Panneerselvam, Lechumi
Phang, Siew Yin
Tan, Wei Theng
Mahyudin, Nor Ainy
In vitro antibacterial activity of crude medicinal plant extracts against ampicillin+penicillin-resistant Staphylococcus aureus
author_facet Samsudin, Nik Iskandar Putra
Lee, Hai Yen
Chern, Pei Ern
Ng, Chew Ting
Panneerselvam, Lechumi
Phang, Siew Yin
Tan, Wei Theng
Mahyudin, Nor Ainy
author_sort Samsudin, Nik Iskandar Putra
title In vitro antibacterial activity of crude medicinal plant extracts against ampicillin+penicillin-resistant Staphylococcus aureus
title_short In vitro antibacterial activity of crude medicinal plant extracts against ampicillin+penicillin-resistant Staphylococcus aureus
title_full In vitro antibacterial activity of crude medicinal plant extracts against ampicillin+penicillin-resistant Staphylococcus aureus
title_fullStr In vitro antibacterial activity of crude medicinal plant extracts against ampicillin+penicillin-resistant Staphylococcus aureus
title_full_unstemmed In vitro antibacterial activity of crude medicinal plant extracts against ampicillin+penicillin-resistant Staphylococcus aureus
title_sort in vitro antibacterial activity of crude medicinal plant extracts against ampicillin+penicillin-resistant staphylococcus aureus
publisher Faculty of Food Science and Technology, Universiti Putra Malaysia
publishDate 2018
url http://psasir.upm.edu.my/id/eprint/64511/1/%2817%29.pdf
http://psasir.upm.edu.my/id/eprint/64511/
http://www.ifrj.upm.edu.my/25%20(02)%202018/(17).pdf
_version_ 1643838045485531136
score 13.211869

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