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Effect of TDZ and BAP on shoot proliferation from in vitro shoot tips of strawberry

An experiment was conducted to induce shoot proliferation from shoot tips derived from runner tips of strawberry cv. Camarosa. The shoot tips were cultured on Murashige and Skoog (MS) medium supplemented with TDZ and BAP, either alone or in combination. The concentrations used were 0 to 8 μM TDZ and...

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Main Authors: Haddadi, Fatemeh, Abdul Aziz, Maheran, Saleh, Ghizan, Abdul Rashid, Azmi, Kamaladini, Hossein
Format: Conference or Workshop Item
Language:English
Published: Sarawak Biodiversity Centre 2008
Online Access:http://psasir.upm.edu.my/id/eprint/64278/1/PROCEEDING_100719-15.pdf
http://psasir.upm.edu.my/id/eprint/64278/
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spelling my.upm.eprints.642782018-07-04T02:31:36Z http://psasir.upm.edu.my/id/eprint/64278/ Effect of TDZ and BAP on shoot proliferation from in vitro shoot tips of strawberry Haddadi, Fatemeh Abdul Aziz, Maheran Saleh, Ghizan Abdul Rashid, Azmi Kamaladini, Hossein An experiment was conducted to induce shoot proliferation from shoot tips derived from runner tips of strawberry cv. Camarosa. The shoot tips were cultured on Murashige and Skoog (MS) medium supplemented with TDZ and BAP, either alone or in combination. The concentrations used were 0 to 8 μM TDZ and 0 to 27 μM BAP. MS medium supplemented with a moderate concentration of 4 μM BAP in combination with 2 μM TDZ was optimum for strawberry shoot proliferation from shoot tips derived from runner tips. In this combination of cytokinins, a 100 % shoot proliferation with a mean number of 14.6 shoots per explant was produced. This regeneration protocol is suitable for mass propagation of planting materials for commercial cultivation of strawberry and for the recovery of transgenic strawberry plants following genetic transformation. Sarawak Biodiversity Centre 2008 Conference or Workshop Item PeerReviewed text en http://psasir.upm.edu.my/id/eprint/64278/1/PROCEEDING_100719-15.pdf Haddadi, Fatemeh and Abdul Aziz, Maheran and Saleh, Ghizan and Abdul Rashid, Azmi and Kamaladini, Hossein (2008) Effect of TDZ and BAP on shoot proliferation from in vitro shoot tips of strawberry. In: Biodiversity and Biotechnology Symposium 2008, 19-21 Nov. 2008, Kuching, Sarawak, Malaysia. (pp. 443-446).
institution Universiti Putra Malaysia
building UPM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Putra Malaysia
content_source UPM Institutional Repository
url_provider http://psasir.upm.edu.my/
language English
description An experiment was conducted to induce shoot proliferation from shoot tips derived from runner tips of strawberry cv. Camarosa. The shoot tips were cultured on Murashige and Skoog (MS) medium supplemented with TDZ and BAP, either alone or in combination. The concentrations used were 0 to 8 μM TDZ and 0 to 27 μM BAP. MS medium supplemented with a moderate concentration of 4 μM BAP in combination with 2 μM TDZ was optimum for strawberry shoot proliferation from shoot tips derived from runner tips. In this combination of cytokinins, a 100 % shoot proliferation with a mean number of 14.6 shoots per explant was produced. This regeneration protocol is suitable for mass propagation of planting materials for commercial cultivation of strawberry and for the recovery of transgenic strawberry plants following genetic transformation.
format Conference or Workshop Item
author Haddadi, Fatemeh
Abdul Aziz, Maheran
Saleh, Ghizan
Abdul Rashid, Azmi
Kamaladini, Hossein
spellingShingle Haddadi, Fatemeh
Abdul Aziz, Maheran
Saleh, Ghizan
Abdul Rashid, Azmi
Kamaladini, Hossein
Effect of TDZ and BAP on shoot proliferation from in vitro shoot tips of strawberry
author_facet Haddadi, Fatemeh
Abdul Aziz, Maheran
Saleh, Ghizan
Abdul Rashid, Azmi
Kamaladini, Hossein
author_sort Haddadi, Fatemeh
title Effect of TDZ and BAP on shoot proliferation from in vitro shoot tips of strawberry
title_short Effect of TDZ and BAP on shoot proliferation from in vitro shoot tips of strawberry
title_full Effect of TDZ and BAP on shoot proliferation from in vitro shoot tips of strawberry
title_fullStr Effect of TDZ and BAP on shoot proliferation from in vitro shoot tips of strawberry
title_full_unstemmed Effect of TDZ and BAP on shoot proliferation from in vitro shoot tips of strawberry
title_sort effect of tdz and bap on shoot proliferation from in vitro shoot tips of strawberry
publisher Sarawak Biodiversity Centre
publishDate 2008
url http://psasir.upm.edu.my/id/eprint/64278/1/PROCEEDING_100719-15.pdf
http://psasir.upm.edu.my/id/eprint/64278/
_version_ 1643837977085870080
score 13.211869

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