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Sustainable anti-MRSA Gracilaria changii extract through molecular biotechnology approach

Global bacterial infection management has a universal trend involving the fight against antibiotic resistant strains. The pressing microbial resistance problem incurred high infectious disease burden and cost constraints, requiring application of newer, more expensive agents. An approach for treatme...

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Main Authors: Shamsudin, Mariana Nor, Mohd Yaakob, Haslinda Ayu, M., Habsah
Format: Conference or Workshop Item
Language:English
Published: Sarawak Biodiversity Centre 2008
Online Access:http://psasir.upm.edu.my/id/eprint/64273/1/PROCEEDING_100719-11.pdf
http://psasir.upm.edu.my/id/eprint/64273/
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spelling my.upm.eprints.642732018-07-04T02:31:42Z http://psasir.upm.edu.my/id/eprint/64273/ Sustainable anti-MRSA Gracilaria changii extract through molecular biotechnology approach Shamsudin, Mariana Nor Mohd Yaakob, Haslinda Ayu M., Habsah Global bacterial infection management has a universal trend involving the fight against antibiotic resistant strains. The pressing microbial resistance problem incurred high infectious disease burden and cost constraints, requiring application of newer, more expensive agents. An approach for treatment against an antibiotic resistant pathogen, MRSA, involving a combination therapy is investigated. In the present study, synergy was determined by the checkerboard assay. The effect in combination of commercial antibiotic and seaweed extract were screened on MRSA strain. Following the disc diffusion test, the checkerboard assay, giving the FIC index of 0.93 indicate that the seaweed extract works in synergy with commercial antibiotics and it has the potential as a resistance modulator. In view of the fact that there was positive effect of the extract, further study were conducted molecularly on several genes to study the effect of the extract in combination with antibiotic toward the selected genes through RT-PCR analysis as compared to effect of the extract alone. The synergy effect on resistant genes of MRSA, namely mecA and mecI showed different nucleotide sequence changes as compared to untreated. The promising application of local G. changii as an antiMRSA agent is an added value to mega-diversity tropical flora. Sarawak Biodiversity Centre 2008 Conference or Workshop Item PeerReviewed text en http://psasir.upm.edu.my/id/eprint/64273/1/PROCEEDING_100719-11.pdf Shamsudin, Mariana Nor and Mohd Yaakob, Haslinda Ayu and M., Habsah (2008) Sustainable anti-MRSA Gracilaria changii extract through molecular biotechnology approach. In: Biodiversity and Biotechnology Symposium 2008, 19-21 Nov. 2008, Kuching, Sarawak, Malaysia. (pp. 297-299).
institution Universiti Putra Malaysia
building UPM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Putra Malaysia
content_source UPM Institutional Repository
url_provider http://psasir.upm.edu.my/
language English
description Global bacterial infection management has a universal trend involving the fight against antibiotic resistant strains. The pressing microbial resistance problem incurred high infectious disease burden and cost constraints, requiring application of newer, more expensive agents. An approach for treatment against an antibiotic resistant pathogen, MRSA, involving a combination therapy is investigated. In the present study, synergy was determined by the checkerboard assay. The effect in combination of commercial antibiotic and seaweed extract were screened on MRSA strain. Following the disc diffusion test, the checkerboard assay, giving the FIC index of 0.93 indicate that the seaweed extract works in synergy with commercial antibiotics and it has the potential as a resistance modulator. In view of the fact that there was positive effect of the extract, further study were conducted molecularly on several genes to study the effect of the extract in combination with antibiotic toward the selected genes through RT-PCR analysis as compared to effect of the extract alone. The synergy effect on resistant genes of MRSA, namely mecA and mecI showed different nucleotide sequence changes as compared to untreated. The promising application of local G. changii as an antiMRSA agent is an added value to mega-diversity tropical flora.
format Conference or Workshop Item
author Shamsudin, Mariana Nor
Mohd Yaakob, Haslinda Ayu
M., Habsah
spellingShingle Shamsudin, Mariana Nor
Mohd Yaakob, Haslinda Ayu
M., Habsah
Sustainable anti-MRSA Gracilaria changii extract through molecular biotechnology approach
author_facet Shamsudin, Mariana Nor
Mohd Yaakob, Haslinda Ayu
M., Habsah
author_sort Shamsudin, Mariana Nor
title Sustainable anti-MRSA Gracilaria changii extract through molecular biotechnology approach
title_short Sustainable anti-MRSA Gracilaria changii extract through molecular biotechnology approach
title_full Sustainable anti-MRSA Gracilaria changii extract through molecular biotechnology approach
title_fullStr Sustainable anti-MRSA Gracilaria changii extract through molecular biotechnology approach
title_full_unstemmed Sustainable anti-MRSA Gracilaria changii extract through molecular biotechnology approach
title_sort sustainable anti-mrsa gracilaria changii extract through molecular biotechnology approach
publisher Sarawak Biodiversity Centre
publishDate 2008
url http://psasir.upm.edu.my/id/eprint/64273/1/PROCEEDING_100719-11.pdf
http://psasir.upm.edu.my/id/eprint/64273/
_version_ 1643837975451140096
score 13.211869

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