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Tips and tricks: antibacterial assay of plant extracts

Antibacterial study of plant origin has brought tremendous breakthrough in various field vis. medicinal, pharmaceutical and food preservatives. The long and tedious process of identifying the capability of extracts due to the need of selecting of smaller sample particle size, maturity of sample, mat...

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Main Authors: Abdullah Sani, Muhamad Shirwan, Bakar, Jamilah, Abdul Rahman, Russly
Format: Conference or Workshop Item
Language:English
Published: Halal Products Research Institute, Universiti Putra Malaysia 2014
Online Access:http://psasir.upm.edu.my/id/eprint/64203/1/M63.pdf
http://psasir.upm.edu.my/id/eprint/64203/
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spelling my.upm.eprints.642032018-07-02T02:58:06Z http://psasir.upm.edu.my/id/eprint/64203/ Tips and tricks: antibacterial assay of plant extracts Abdullah Sani, Muhamad Shirwan Bakar, Jamilah Abdul Rahman, Russly Antibacterial study of plant origin has brought tremendous breakthrough in various field vis. medicinal, pharmaceutical and food preservatives. The long and tedious process of identifying the capability of extracts due to the need of selecting of smaller sample particle size, maturity of sample, matrix interference, appropriate solvent polarity, substrate to solvent ratio, extraction technique, and sample storage and temperature promises high turnout of antibacterial capacity. Other consideration for instance extraction temperature, duration time, added process such as sonication, sample pretreatment affect the antibacterial extraction. Solvents used in extraction have bactericidal effect on pathogens tested. Agar diffusion and broth dilution are endpoint methods while descriptive methods involve turbidity assays and inhibition curves are used in antibacterial evaluation. Broth dilution method by spectroscopy instrument involves microscale and macroscale volume up to 250 μL and 1 mL respectively. Soluble concentration equivalent to lower concentration of non-polar extract reduces effect of precipitation in minimum inhibitory concentration (MIC) test. MIC is easily determined by polar extracts since it is immiscible with dimethyl sulfoxide (DMSO) and broth media. Minimum bactericidal concentration (MBC) is identified at higher concentration than MIC. Determination of MIC0, MIC50 and MIC100 can be obtained from turbidity assays. Lethality of pathogens can be established at concentration lower than MIC through inhibition on profile curves. Each test should include negative (solvent and DMSO) and positive (penicillin or tetracycline) measures to support the data analysis. Complementary antibacterial assays are recommended for confirmation of antibacterial properties from plant extracts. Halal Products Research Institute, Universiti Putra Malaysia 2014 Conference or Workshop Item PeerReviewed text en http://psasir.upm.edu.my/id/eprint/64203/1/M63.pdf Abdullah Sani, Muhamad Shirwan and Bakar, Jamilah and Abdul Rahman, Russly (2014) Tips and tricks: antibacterial assay of plant extracts. In: Malaysia International Halal Research & Education Conference 2014 (MIHREC 2014), 2-4 Dec. 2014, Marriott Putrajaya Hotel, Malaysia. (p. 304).
institution Universiti Putra Malaysia
building UPM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Putra Malaysia
content_source UPM Institutional Repository
url_provider http://psasir.upm.edu.my/
language English
description Antibacterial study of plant origin has brought tremendous breakthrough in various field vis. medicinal, pharmaceutical and food preservatives. The long and tedious process of identifying the capability of extracts due to the need of selecting of smaller sample particle size, maturity of sample, matrix interference, appropriate solvent polarity, substrate to solvent ratio, extraction technique, and sample storage and temperature promises high turnout of antibacterial capacity. Other consideration for instance extraction temperature, duration time, added process such as sonication, sample pretreatment affect the antibacterial extraction. Solvents used in extraction have bactericidal effect on pathogens tested. Agar diffusion and broth dilution are endpoint methods while descriptive methods involve turbidity assays and inhibition curves are used in antibacterial evaluation. Broth dilution method by spectroscopy instrument involves microscale and macroscale volume up to 250 μL and 1 mL respectively. Soluble concentration equivalent to lower concentration of non-polar extract reduces effect of precipitation in minimum inhibitory concentration (MIC) test. MIC is easily determined by polar extracts since it is immiscible with dimethyl sulfoxide (DMSO) and broth media. Minimum bactericidal concentration (MBC) is identified at higher concentration than MIC. Determination of MIC0, MIC50 and MIC100 can be obtained from turbidity assays. Lethality of pathogens can be established at concentration lower than MIC through inhibition on profile curves. Each test should include negative (solvent and DMSO) and positive (penicillin or tetracycline) measures to support the data analysis. Complementary antibacterial assays are recommended for confirmation of antibacterial properties from plant extracts.
format Conference or Workshop Item
author Abdullah Sani, Muhamad Shirwan
Bakar, Jamilah
Abdul Rahman, Russly
spellingShingle Abdullah Sani, Muhamad Shirwan
Bakar, Jamilah
Abdul Rahman, Russly
Tips and tricks: antibacterial assay of plant extracts
author_facet Abdullah Sani, Muhamad Shirwan
Bakar, Jamilah
Abdul Rahman, Russly
author_sort Abdullah Sani, Muhamad Shirwan
title Tips and tricks: antibacterial assay of plant extracts
title_short Tips and tricks: antibacterial assay of plant extracts
title_full Tips and tricks: antibacterial assay of plant extracts
title_fullStr Tips and tricks: antibacterial assay of plant extracts
title_full_unstemmed Tips and tricks: antibacterial assay of plant extracts
title_sort tips and tricks: antibacterial assay of plant extracts
publisher Halal Products Research Institute, Universiti Putra Malaysia
publishDate 2014
url http://psasir.upm.edu.my/id/eprint/64203/1/M63.pdf
http://psasir.upm.edu.my/id/eprint/64203/
_version_ 1643837955750494208
score 13.211869

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