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Assay for heavy metals using an inhibitive assay based on the acetylcholinesterase from Clarias batrachus

Acetylcholinesterase (AChE) is usually used as an inhibitive assay for insecticides. A lesser-known property of AChE is its inhibition by heavy metals. In this work, we evaluate an AChE from brains of Clarias batrachus (catfish) exposed to wastes from aquaculture industry as an inhibitive assay for...

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Main Authors: Muhammad, Umar Abubakar, Tham, Lik Gin, Perumal, Natarajan, Mohd Daud, Hassan, Yasid, Nur Adeela, Abd. Shukor, Mohd. Yunus
Format: Article
Language:English
Published: Hibiscus Publisher 2016
Online Access:http://psasir.upm.edu.my/id/eprint/64020/1/Assay%20for%20heavy%20metals%20using%20an%20inhibitive%20assay%20based%20on%20the%20acetylcholinesterase%20from%20Clarias%20batrachus.pdf
http://psasir.upm.edu.my/id/eprint/64020/
https://journal.hibiscuspublisher.com/index.php/BSTR/article/view/369
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spelling my.upm.eprints.640202018-06-08T00:51:57Z http://psasir.upm.edu.my/id/eprint/64020/ Assay for heavy metals using an inhibitive assay based on the acetylcholinesterase from Clarias batrachus Muhammad, Umar Abubakar Tham, Lik Gin Perumal, Natarajan Mohd Daud, Hassan Yasid, Nur Adeela Abd. Shukor, Mohd. Yunus Acetylcholinesterase (AChE) is usually used as an inhibitive assay for insecticides. A lesser-known property of AChE is its inhibition by heavy metals. In this work, we evaluate an AChE from brains of Clarias batrachus (catfish) exposed to wastes from aquaculture industry as an inhibitive assay for heavy metals. We discovered that the AChE was inhibited completely by Hg2+, Ag2+, Pb2+, Cu2+, Cd2+, Cr6+ and Zn2+ during initial screening. When tested at various concentrations, the heavy metals exhibited exponential decay type inhibition curves. The calculated IC50 (mg/L) for the heavy metals Ag2+, Cu2+, Hg2+, Cr6+ and Cd2+ were 0.088, 0.078, 0.071, 0.87 and 0.913, respectively. The IC50 for these heavy metals are comparable, and some are lower than the IC50 values from the cholinesterases from previously studied fish. The assay can be carried out in less than 30 minutes at ambient temperature. Hibiscus Publisher 2016 Article PeerReviewed text en http://psasir.upm.edu.my/id/eprint/64020/1/Assay%20for%20heavy%20metals%20using%20an%20inhibitive%20assay%20based%20on%20the%20acetylcholinesterase%20from%20Clarias%20batrachus.pdf Muhammad, Umar Abubakar and Tham, Lik Gin and Perumal, Natarajan and Mohd Daud, Hassan and Yasid, Nur Adeela and Abd. Shukor, Mohd. Yunus (2016) Assay for heavy metals using an inhibitive assay based on the acetylcholinesterase from Clarias batrachus. Bioremediation Science and Technology Research, 4 (2). pp. 6-10. ISSN 2289-5892 https://journal.hibiscuspublisher.com/index.php/BSTR/article/view/369
institution Universiti Putra Malaysia
building UPM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Putra Malaysia
content_source UPM Institutional Repository
url_provider http://psasir.upm.edu.my/
language English
description Acetylcholinesterase (AChE) is usually used as an inhibitive assay for insecticides. A lesser-known property of AChE is its inhibition by heavy metals. In this work, we evaluate an AChE from brains of Clarias batrachus (catfish) exposed to wastes from aquaculture industry as an inhibitive assay for heavy metals. We discovered that the AChE was inhibited completely by Hg2+, Ag2+, Pb2+, Cu2+, Cd2+, Cr6+ and Zn2+ during initial screening. When tested at various concentrations, the heavy metals exhibited exponential decay type inhibition curves. The calculated IC50 (mg/L) for the heavy metals Ag2+, Cu2+, Hg2+, Cr6+ and Cd2+ were 0.088, 0.078, 0.071, 0.87 and 0.913, respectively. The IC50 for these heavy metals are comparable, and some are lower than the IC50 values from the cholinesterases from previously studied fish. The assay can be carried out in less than 30 minutes at ambient temperature.
format Article
author Muhammad, Umar Abubakar
Tham, Lik Gin
Perumal, Natarajan
Mohd Daud, Hassan
Yasid, Nur Adeela
Abd. Shukor, Mohd. Yunus
spellingShingle Muhammad, Umar Abubakar
Tham, Lik Gin
Perumal, Natarajan
Mohd Daud, Hassan
Yasid, Nur Adeela
Abd. Shukor, Mohd. Yunus
Assay for heavy metals using an inhibitive assay based on the acetylcholinesterase from Clarias batrachus
author_facet Muhammad, Umar Abubakar
Tham, Lik Gin
Perumal, Natarajan
Mohd Daud, Hassan
Yasid, Nur Adeela
Abd. Shukor, Mohd. Yunus
author_sort Muhammad, Umar Abubakar
title Assay for heavy metals using an inhibitive assay based on the acetylcholinesterase from Clarias batrachus
title_short Assay for heavy metals using an inhibitive assay based on the acetylcholinesterase from Clarias batrachus
title_full Assay for heavy metals using an inhibitive assay based on the acetylcholinesterase from Clarias batrachus
title_fullStr Assay for heavy metals using an inhibitive assay based on the acetylcholinesterase from Clarias batrachus
title_full_unstemmed Assay for heavy metals using an inhibitive assay based on the acetylcholinesterase from Clarias batrachus
title_sort assay for heavy metals using an inhibitive assay based on the acetylcholinesterase from clarias batrachus
publisher Hibiscus Publisher
publishDate 2016
url http://psasir.upm.edu.my/id/eprint/64020/1/Assay%20for%20heavy%20metals%20using%20an%20inhibitive%20assay%20based%20on%20the%20acetylcholinesterase%20from%20Clarias%20batrachus.pdf
http://psasir.upm.edu.my/id/eprint/64020/
https://journal.hibiscuspublisher.com/index.php/BSTR/article/view/369
_version_ 1643837903099396096
score 13.211869

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