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Isolation and characterisation of a Mo-reducing bacterium from Malaysian soil

The issue of heavy metal contamination and toxic xenobiotics has become a rapid global concern. This has ensured that the bioremediation of these toxicants, which are being carried out using novel microbes. A bacterium with the ability to reduce molybdenum has been isolated from contaminated soils a...

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Main Authors: Chee, Hui Sin, Manogaran, Motharasan, Suhaili, Zarizal, Yakasai, Mohd Hafeez, Abdul Rahman, Mohd Fadhil, Shamaan, Nor Aripin, Yasid, Nur Adeela, Othman, Ahmad Razi
Format: Article
Language:English
Published: Hibiscus Publisher 2017
Online Access:http://psasir.upm.edu.my/id/eprint/64004/1/Isolation%20and%20characterisation%20of%20a%20Mo-reducing%20bacterium%20from%20Malaysian%20soil.pdf
http://psasir.upm.edu.my/id/eprint/64004/
https://journal.hibiscuspublisher.com/index.php/BSTR/article/view/359
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spelling my.upm.eprints.640042018-06-08T00:45:04Z http://psasir.upm.edu.my/id/eprint/64004/ Isolation and characterisation of a Mo-reducing bacterium from Malaysian soil Chee, Hui Sin Manogaran, Motharasan Suhaili, Zarizal Yakasai, Mohd Hafeez Abdul Rahman, Mohd Fadhil Shamaan, Nor Aripin Yasid, Nur Adeela Othman, Ahmad Razi The issue of heavy metal contamination and toxic xenobiotics has become a rapid global concern. This has ensured that the bioremediation of these toxicants, which are being carried out using novel microbes. A bacterium with the ability to reduce molybdenum has been isolated from contaminated soils and identified as Serratia marcescens strain DR.Y10. The bacterium reduced molybdenum (sodium molybdate) to molybdenum blue (Mo-blue) optimally at pHs of between 6.0 and 6.5 and temperatures between 30°C and 37°C. Glucose was the best electron donor for supporting molybdate reduction followed by sucrose, adonitol, mannose, maltose, mannitol glycerol, salicin, myo-inositol, sorbitol and trehalose in descending order. Other requirements include a phosphate concentration of 5 mM and a molybdate concentration of between 10 and 30 mM. The absorption spectrum of the Mo-blue produced was similar to the previously isolated Mo-reducing bacterium and closely resembles a reduced phosphomolybdate. Molybdenum reduction was inhibited by Hg (ii), Ag (i), Cu (ii), and Cr (vi) at 78.9, 69.2, 59.5 and 40.1%, respectively. We also screen for the ability of the bacterium to use various organic xenobiotics such as phenol, acrylamide, nicotinamide, acetamide, iodoacetamide, propionamide, acetamide, sodium dodecyl sulfate (SDS) and diesel as electron donor sources for aiding reduction. The bacterium was also able to grow using amides such as acrylamide, propionamide and acetamide without molybdenum reduction. The unique ability of the bacterium to detoxify many toxicants is much in demand, making this bacterium a vital means of bioremediation. Hibiscus Publisher 2017 Article PeerReviewed text en http://psasir.upm.edu.my/id/eprint/64004/1/Isolation%20and%20characterisation%20of%20a%20Mo-reducing%20bacterium%20from%20Malaysian%20soil.pdf Chee, Hui Sin and Manogaran, Motharasan and Suhaili, Zarizal and Yakasai, Mohd Hafeez and Abdul Rahman, Mohd Fadhil and Shamaan, Nor Aripin and Yasid, Nur Adeela and Othman, Ahmad Razi (2017) Isolation and characterisation of a Mo-reducing bacterium from Malaysian soil. Bioremediation Science and Technology Research, 5 (2). pp. 17-24. ISSN 2289-5892 https://journal.hibiscuspublisher.com/index.php/BSTR/article/view/359
institution Universiti Putra Malaysia
building UPM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Putra Malaysia
content_source UPM Institutional Repository
url_provider http://psasir.upm.edu.my/
language English
description The issue of heavy metal contamination and toxic xenobiotics has become a rapid global concern. This has ensured that the bioremediation of these toxicants, which are being carried out using novel microbes. A bacterium with the ability to reduce molybdenum has been isolated from contaminated soils and identified as Serratia marcescens strain DR.Y10. The bacterium reduced molybdenum (sodium molybdate) to molybdenum blue (Mo-blue) optimally at pHs of between 6.0 and 6.5 and temperatures between 30°C and 37°C. Glucose was the best electron donor for supporting molybdate reduction followed by sucrose, adonitol, mannose, maltose, mannitol glycerol, salicin, myo-inositol, sorbitol and trehalose in descending order. Other requirements include a phosphate concentration of 5 mM and a molybdate concentration of between 10 and 30 mM. The absorption spectrum of the Mo-blue produced was similar to the previously isolated Mo-reducing bacterium and closely resembles a reduced phosphomolybdate. Molybdenum reduction was inhibited by Hg (ii), Ag (i), Cu (ii), and Cr (vi) at 78.9, 69.2, 59.5 and 40.1%, respectively. We also screen for the ability of the bacterium to use various organic xenobiotics such as phenol, acrylamide, nicotinamide, acetamide, iodoacetamide, propionamide, acetamide, sodium dodecyl sulfate (SDS) and diesel as electron donor sources for aiding reduction. The bacterium was also able to grow using amides such as acrylamide, propionamide and acetamide without molybdenum reduction. The unique ability of the bacterium to detoxify many toxicants is much in demand, making this bacterium a vital means of bioremediation.
format Article
author Chee, Hui Sin
Manogaran, Motharasan
Suhaili, Zarizal
Yakasai, Mohd Hafeez
Abdul Rahman, Mohd Fadhil
Shamaan, Nor Aripin
Yasid, Nur Adeela
Othman, Ahmad Razi
spellingShingle Chee, Hui Sin
Manogaran, Motharasan
Suhaili, Zarizal
Yakasai, Mohd Hafeez
Abdul Rahman, Mohd Fadhil
Shamaan, Nor Aripin
Yasid, Nur Adeela
Othman, Ahmad Razi
Isolation and characterisation of a Mo-reducing bacterium from Malaysian soil
author_facet Chee, Hui Sin
Manogaran, Motharasan
Suhaili, Zarizal
Yakasai, Mohd Hafeez
Abdul Rahman, Mohd Fadhil
Shamaan, Nor Aripin
Yasid, Nur Adeela
Othman, Ahmad Razi
author_sort Chee, Hui Sin
title Isolation and characterisation of a Mo-reducing bacterium from Malaysian soil
title_short Isolation and characterisation of a Mo-reducing bacterium from Malaysian soil
title_full Isolation and characterisation of a Mo-reducing bacterium from Malaysian soil
title_fullStr Isolation and characterisation of a Mo-reducing bacterium from Malaysian soil
title_full_unstemmed Isolation and characterisation of a Mo-reducing bacterium from Malaysian soil
title_sort isolation and characterisation of a mo-reducing bacterium from malaysian soil
publisher Hibiscus Publisher
publishDate 2017
url http://psasir.upm.edu.my/id/eprint/64004/1/Isolation%20and%20characterisation%20of%20a%20Mo-reducing%20bacterium%20from%20Malaysian%20soil.pdf
http://psasir.upm.edu.my/id/eprint/64004/
https://journal.hibiscuspublisher.com/index.php/BSTR/article/view/359
_version_ 1643837898899849216
score 13.211869

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