Cover Image

Urinary metabolic profiling of cisplatin nephrotoxicity and nephroprotective effects of Orthosiphon stamineus leaves elucidated by 1H NMR spectroscopy

Orthosiphon stamineus (OS) is a popular medicinal herb used in traditional Chinese medicine as a diuretic agent and for renal system disorders. This study employed 1H NMR based metabolomics approach to investigate the possible protective activity of OS in cisplatin induced nephrotoxicity owing to it...

Full description

Saved in:
Bibliographic Details
Main Authors: Pariyani, Raghunath, Ismail, Intan Safinar, Azam, Amalina, Khatib, Alfi, Abas, Faridah, Shaari, Khozirah, Hamzah, Hazilawati
Format: Article
Language:English
Published: Elsevier 2017
Online Access:http://psasir.upm.edu.my/id/eprint/63647/1/Urinary%20metabolic%20profiling%20of%20cisplatin%20nephrotoxicity%20and%20nephroprotective%20effects%20of%20Orthosiphon%20stamineus%20leaves%20elucidated%20by%201H%20NMR%20spectroscopy.pdf
http://psasir.upm.edu.my/id/eprint/63647/
https://www.sciencedirect.com/science/article/pii/S0731708516313292?via%3Dihub
Tags: Add Tag
No Tags, Be the first to tag this record!
id my.upm.eprints.63647
record_format eprints
spelling my.upm.eprints.636472018-11-07T08:52:05Z http://psasir.upm.edu.my/id/eprint/63647/ Urinary metabolic profiling of cisplatin nephrotoxicity and nephroprotective effects of Orthosiphon stamineus leaves elucidated by 1H NMR spectroscopy Pariyani, Raghunath Ismail, Intan Safinar Azam, Amalina Khatib, Alfi Abas, Faridah Shaari, Khozirah Hamzah, Hazilawati Orthosiphon stamineus (OS) is a popular medicinal herb used in traditional Chinese medicine as a diuretic agent and for renal system disorders. This study employed 1H NMR based metabolomics approach to investigate the possible protective activity of OS in cisplatin induced nephrotoxicity owing to its diuretic and antioxidant activities. Aqueous (OSAE) and 50% aqueous ethanolic (OSFE) extracts of OS leaves were orally administered at 400 mg/kg BW doses to rats which were then intraperitoneally injected with cisplatin at 5 mg/kg BW dose. The 1H NMR profile of the urine samples collected on day 5 after cisplatin administration were analyzed by multivariate pattern recognition techniques, whereby 19 marker metabolites suggestive in the involvement of TCA cycle, disturbed energy metabolism, altered gut microflora and BCAA metabolism pathways were identified. It was observed that OSFE caused significant changes (p < 0.05) in the levels of 8 markers namely leucine, acetate, hippurate, lysine, valine, 2-oxoglutarate, 3-HBT and acetoacetate resulting in a moderate ameliorative effect, however, it did not completely protect from nephrotoxicity. OSAE did not demonstrate significant down regulatory effects on any markers, albeit, it potentiated the cisplatin nephrotoxicity by inducing significant increase in glucose, glycine, creatinine, citrate, TMAO, acetate and creatine levels. A Principal Component Analysis (PCA) of the 1H NMR spectra of OS extracts identified that OSFE had higher concentrations of the secondary metabolites such as caffeic acid, chlorogenic acid, protocatechuic acid and orthosiphol, among others. Whereas, OSAE was characterized by higher concentrations of acetate, lactate, succinic acid, valine and phosphatidylcholine. This research denotes the first comprehensive analysis to identify the effects of OS extracts on cisplatin nephrotoxicity. Elsevier 2017-02-20 Article PeerReviewed text en http://psasir.upm.edu.my/id/eprint/63647/1/Urinary%20metabolic%20profiling%20of%20cisplatin%20nephrotoxicity%20and%20nephroprotective%20effects%20of%20Orthosiphon%20stamineus%20leaves%20elucidated%20by%201H%20NMR%20spectroscopy.pdf Pariyani, Raghunath and Ismail, Intan Safinar and Azam, Amalina and Khatib, Alfi and Abas, Faridah and Shaari, Khozirah and Hamzah, Hazilawati (2017) Urinary metabolic profiling of cisplatin nephrotoxicity and nephroprotective effects of Orthosiphon stamineus leaves elucidated by 1H NMR spectroscopy. Journal of Pharmaceutical and Biomedical Analysis, 135. 20 - 30. ISSN 0731-7085; ESSN: 1873-264X https://www.sciencedirect.com/science/article/pii/S0731708516313292?via%3Dihub 10.1016/j.jpba.2016.12.010
institution Universiti Putra Malaysia
building UPM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Putra Malaysia
content_source UPM Institutional Repository
url_provider http://psasir.upm.edu.my/
language English
description Orthosiphon stamineus (OS) is a popular medicinal herb used in traditional Chinese medicine as a diuretic agent and for renal system disorders. This study employed 1H NMR based metabolomics approach to investigate the possible protective activity of OS in cisplatin induced nephrotoxicity owing to its diuretic and antioxidant activities. Aqueous (OSAE) and 50% aqueous ethanolic (OSFE) extracts of OS leaves were orally administered at 400 mg/kg BW doses to rats which were then intraperitoneally injected with cisplatin at 5 mg/kg BW dose. The 1H NMR profile of the urine samples collected on day 5 after cisplatin administration were analyzed by multivariate pattern recognition techniques, whereby 19 marker metabolites suggestive in the involvement of TCA cycle, disturbed energy metabolism, altered gut microflora and BCAA metabolism pathways were identified. It was observed that OSFE caused significant changes (p < 0.05) in the levels of 8 markers namely leucine, acetate, hippurate, lysine, valine, 2-oxoglutarate, 3-HBT and acetoacetate resulting in a moderate ameliorative effect, however, it did not completely protect from nephrotoxicity. OSAE did not demonstrate significant down regulatory effects on any markers, albeit, it potentiated the cisplatin nephrotoxicity by inducing significant increase in glucose, glycine, creatinine, citrate, TMAO, acetate and creatine levels. A Principal Component Analysis (PCA) of the 1H NMR spectra of OS extracts identified that OSFE had higher concentrations of the secondary metabolites such as caffeic acid, chlorogenic acid, protocatechuic acid and orthosiphol, among others. Whereas, OSAE was characterized by higher concentrations of acetate, lactate, succinic acid, valine and phosphatidylcholine. This research denotes the first comprehensive analysis to identify the effects of OS extracts on cisplatin nephrotoxicity.
format Article
author Pariyani, Raghunath
Ismail, Intan Safinar
Azam, Amalina
Khatib, Alfi
Abas, Faridah
Shaari, Khozirah
Hamzah, Hazilawati
spellingShingle Pariyani, Raghunath
Ismail, Intan Safinar
Azam, Amalina
Khatib, Alfi
Abas, Faridah
Shaari, Khozirah
Hamzah, Hazilawati
Urinary metabolic profiling of cisplatin nephrotoxicity and nephroprotective effects of Orthosiphon stamineus leaves elucidated by 1H NMR spectroscopy
author_facet Pariyani, Raghunath
Ismail, Intan Safinar
Azam, Amalina
Khatib, Alfi
Abas, Faridah
Shaari, Khozirah
Hamzah, Hazilawati
author_sort Pariyani, Raghunath
title Urinary metabolic profiling of cisplatin nephrotoxicity and nephroprotective effects of Orthosiphon stamineus leaves elucidated by 1H NMR spectroscopy
title_short Urinary metabolic profiling of cisplatin nephrotoxicity and nephroprotective effects of Orthosiphon stamineus leaves elucidated by 1H NMR spectroscopy
title_full Urinary metabolic profiling of cisplatin nephrotoxicity and nephroprotective effects of Orthosiphon stamineus leaves elucidated by 1H NMR spectroscopy
title_fullStr Urinary metabolic profiling of cisplatin nephrotoxicity and nephroprotective effects of Orthosiphon stamineus leaves elucidated by 1H NMR spectroscopy
title_full_unstemmed Urinary metabolic profiling of cisplatin nephrotoxicity and nephroprotective effects of Orthosiphon stamineus leaves elucidated by 1H NMR spectroscopy
title_sort urinary metabolic profiling of cisplatin nephrotoxicity and nephroprotective effects of orthosiphon stamineus leaves elucidated by 1h nmr spectroscopy
publisher Elsevier
publishDate 2017
url http://psasir.upm.edu.my/id/eprint/63647/1/Urinary%20metabolic%20profiling%20of%20cisplatin%20nephrotoxicity%20and%20nephroprotective%20effects%20of%20Orthosiphon%20stamineus%20leaves%20elucidated%20by%201H%20NMR%20spectroscopy.pdf
http://psasir.upm.edu.my/id/eprint/63647/
https://www.sciencedirect.com/science/article/pii/S0731708516313292?via%3Dihub
_version_ 1643837851862827008
score 13.223943

Similar Items