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The presence of vibrionaceae, betanodavirus and iridovirus in marine cage-cultured fish: role of fish size, water physicochemical parameters and relationships among the pathogens

The study determines the presence of Vibrionaceae, Betanodavirus and Iridovirus in marine cage-cultured fish, while identifying the roles of fish size, water physicochemical parameters and relationships among the pathogens itself. Cultured grouper and snapper were randomly sampled from a commercial...

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Main Authors: Abdullah, Azila, Ramli, Rimatulhana, Ridzuan, Mohd Syafiq Mohammad, Murni, Munira, Hashim, Shahidan, Sudirwan, Fahmi, Abdullah, Siti Zahrah, Mansor, Nur Nazifah, Amira, Sofea, Saad, Mohd Zamri, Amal, Mohammad Noor Azmai
Format: Article
Language:English
Published: Elsevier 2017
Online Access:http://psasir.upm.edu.my/id/eprint/63503/1/The%20presence%20of%20Vibrionaceae%2C%20Betanodavirus%20and%20Iridovirus%20in%20marine%20cage-cultured%20fish.pdf
http://psasir.upm.edu.my/id/eprint/63503/
https://www.sciencedirect.com/science/article/pii/S2352513417300388
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spelling my.upm.eprints.635032018-10-16T09:45:57Z http://psasir.upm.edu.my/id/eprint/63503/ The presence of vibrionaceae, betanodavirus and iridovirus in marine cage-cultured fish: role of fish size, water physicochemical parameters and relationships among the pathogens Abdullah, Azila Ramli, Rimatulhana Ridzuan, Mohd Syafiq Mohammad Murni, Munira Hashim, Shahidan Sudirwan, Fahmi Abdullah, Siti Zahrah Mansor, Nur Nazifah Amira, Sofea Saad, Mohd Zamri Amal, Mohammad Noor Azmai The study determines the presence of Vibrionaceae, Betanodavirus and Iridovirus in marine cage-cultured fish, while identifying the roles of fish size, water physicochemical parameters and relationships among the pathogens itself. Cultured grouper and snapper were randomly sampled from a commercial fish farm between February and December 2014. The total body weight and length of individual fish were measured. The kidney, liver and spleen were sampled for bacteria isolation, while for viral identification, the sample of brain, eye, kidney and spleen were used. Water physicochemical parameters during the sampling activities were also determined. Laboratory results revealed isolations of multiple pathogens including Vibrio alginolyticus, V. vulnificus, Photobacterium damselae subsp. damselae (PD), Nervous Necrosis Virus (NNV) and Iridovirus (IV) at low to high prevalence throughout the study period. The weight of affected groupers ranged between 98 g and 719 g, while snappers between 67 g and 982 g. There was a weak and moderate negative correlation between the grouper’s weight and the presence of NNV (R = −0.3684; P < 0.05) and V. vulnificus (R = −0.6451; P < 0.05), respectively. No significant (P > 0.05) difference was noted in the rate of isolated pathogens between groupers and snappers, and between the pathogens affecting snappers. However, detection of IV in groupers was significantly (P < 0.05) higher than V. alginolyticus, V. vulnificus and PD. Isolations of V. vulnificus in groupers showed strong and moderate positive correlations with isolations of PD (R = 0.7069; P < 0.05) and IV (R = 0.6665; P < 0.05), respectively. In snappers, there was strong positive correlation between isolation of V. alginolyticus and NNV (R = 0.7526; P < 0.05). Multivariate analyses showed that water temperature, dissolved oxygen, ammonia, iron and nitrite were the most significant water physicochemical parameters associated with presence of these pathogens. Elsevier 2017-08 Article PeerReviewed text en http://psasir.upm.edu.my/id/eprint/63503/1/The%20presence%20of%20Vibrionaceae%2C%20Betanodavirus%20and%20Iridovirus%20in%20marine%20cage-cultured%20fish.pdf Abdullah, Azila and Ramli, Rimatulhana and Ridzuan, Mohd Syafiq Mohammad and Murni, Munira and Hashim, Shahidan and Sudirwan, Fahmi and Abdullah, Siti Zahrah and Mansor, Nur Nazifah and Amira, Sofea and Saad, Mohd Zamri and Amal, Mohammad Noor Azmai (2017) The presence of vibrionaceae, betanodavirus and iridovirus in marine cage-cultured fish: role of fish size, water physicochemical parameters and relationships among the pathogens. Aquaculture Reports, 7. pp. 57-65. ISSN 2352-5134 https://www.sciencedirect.com/science/article/pii/S2352513417300388 10.1016/j.aqrep.2017.06.001
institution Universiti Putra Malaysia
building UPM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Putra Malaysia
content_source UPM Institutional Repository
url_provider http://psasir.upm.edu.my/
language English
description The study determines the presence of Vibrionaceae, Betanodavirus and Iridovirus in marine cage-cultured fish, while identifying the roles of fish size, water physicochemical parameters and relationships among the pathogens itself. Cultured grouper and snapper were randomly sampled from a commercial fish farm between February and December 2014. The total body weight and length of individual fish were measured. The kidney, liver and spleen were sampled for bacteria isolation, while for viral identification, the sample of brain, eye, kidney and spleen were used. Water physicochemical parameters during the sampling activities were also determined. Laboratory results revealed isolations of multiple pathogens including Vibrio alginolyticus, V. vulnificus, Photobacterium damselae subsp. damselae (PD), Nervous Necrosis Virus (NNV) and Iridovirus (IV) at low to high prevalence throughout the study period. The weight of affected groupers ranged between 98 g and 719 g, while snappers between 67 g and 982 g. There was a weak and moderate negative correlation between the grouper’s weight and the presence of NNV (R = −0.3684; P < 0.05) and V. vulnificus (R = −0.6451; P < 0.05), respectively. No significant (P > 0.05) difference was noted in the rate of isolated pathogens between groupers and snappers, and between the pathogens affecting snappers. However, detection of IV in groupers was significantly (P < 0.05) higher than V. alginolyticus, V. vulnificus and PD. Isolations of V. vulnificus in groupers showed strong and moderate positive correlations with isolations of PD (R = 0.7069; P < 0.05) and IV (R = 0.6665; P < 0.05), respectively. In snappers, there was strong positive correlation between isolation of V. alginolyticus and NNV (R = 0.7526; P < 0.05). Multivariate analyses showed that water temperature, dissolved oxygen, ammonia, iron and nitrite were the most significant water physicochemical parameters associated with presence of these pathogens.
format Article
author Abdullah, Azila
Ramli, Rimatulhana
Ridzuan, Mohd Syafiq Mohammad
Murni, Munira
Hashim, Shahidan
Sudirwan, Fahmi
Abdullah, Siti Zahrah
Mansor, Nur Nazifah
Amira, Sofea
Saad, Mohd Zamri
Amal, Mohammad Noor Azmai
spellingShingle Abdullah, Azila
Ramli, Rimatulhana
Ridzuan, Mohd Syafiq Mohammad
Murni, Munira
Hashim, Shahidan
Sudirwan, Fahmi
Abdullah, Siti Zahrah
Mansor, Nur Nazifah
Amira, Sofea
Saad, Mohd Zamri
Amal, Mohammad Noor Azmai
The presence of vibrionaceae, betanodavirus and iridovirus in marine cage-cultured fish: role of fish size, water physicochemical parameters and relationships among the pathogens
author_facet Abdullah, Azila
Ramli, Rimatulhana
Ridzuan, Mohd Syafiq Mohammad
Murni, Munira
Hashim, Shahidan
Sudirwan, Fahmi
Abdullah, Siti Zahrah
Mansor, Nur Nazifah
Amira, Sofea
Saad, Mohd Zamri
Amal, Mohammad Noor Azmai
author_sort Abdullah, Azila
title The presence of vibrionaceae, betanodavirus and iridovirus in marine cage-cultured fish: role of fish size, water physicochemical parameters and relationships among the pathogens
title_short The presence of vibrionaceae, betanodavirus and iridovirus in marine cage-cultured fish: role of fish size, water physicochemical parameters and relationships among the pathogens
title_full The presence of vibrionaceae, betanodavirus and iridovirus in marine cage-cultured fish: role of fish size, water physicochemical parameters and relationships among the pathogens
title_fullStr The presence of vibrionaceae, betanodavirus and iridovirus in marine cage-cultured fish: role of fish size, water physicochemical parameters and relationships among the pathogens
title_full_unstemmed The presence of vibrionaceae, betanodavirus and iridovirus in marine cage-cultured fish: role of fish size, water physicochemical parameters and relationships among the pathogens
title_sort presence of vibrionaceae, betanodavirus and iridovirus in marine cage-cultured fish: role of fish size, water physicochemical parameters and relationships among the pathogens
publisher Elsevier
publishDate 2017
url http://psasir.upm.edu.my/id/eprint/63503/1/The%20presence%20of%20Vibrionaceae%2C%20Betanodavirus%20and%20Iridovirus%20in%20marine%20cage-cultured%20fish.pdf
http://psasir.upm.edu.my/id/eprint/63503/
https://www.sciencedirect.com/science/article/pii/S2352513417300388
_version_ 1643837817907838976
score 13.211869

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