Comparison of Sybr Green I, ELISA and conventional agarose gel-based PCR in the detection of infectious bursal disease virus
The current available molecular method to detect infectious bursal disease virus (IBDV) is by reverse transcriptase-polymerase chain reaction (RT-PCR). However, the conventional PCR is time consuming, prone to error and less sensitive. In this study, the performances of Sybr Green I real-time PCR, e...
Saved in:
Main Authors: | Hamzah, Hairul Aini, Omar, Abdul Rahman, Bejo, Mohd Hair, Ideris, Aini |
---|---|
Format: | Article |
Language: | English English |
Published: |
Elsevier
2008
|
Online Access: | http://psasir.upm.edu.my/id/eprint/6343/1/Comparison%20of%20Sybr%20Green%20I.pdf http://psasir.upm.edu.my/id/eprint/6343/ http://dx.doi.org/10.1016/j.micres.2006.08.003 |
Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
Similar Items
-
Detection of infectious bursal disease virus using SYBR Green 1 based realtime polymerase chain reaction
by: Hamzah, Hairul Aini, et al.
Published: (2004) -
In situ PCR for detection and differentiation of infectious bursal disease virus strains in chickens
by: Hussein, Elawad A., et al.
Published: (2017) -
Pathogenicity and immunogenicity of infectious bursal disease virus in poultry
by: Bejo, Mohd Hair, et al.
Published: (1999) -
Development of SYBR green I based one-step real-time RT-PCR assay for the detection and differentiation of very virulent and classical strains of infectious bursal disease virus
by: Lih, Ling Kong, et al.
Published: (2009) -
Development of in situ PCR technique for detection of very virulent infectious bursal disease virus strain
by: Elawad, H. A., et al.
Published: (2013)