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Strategies for improving production performance of probiotic Pediococcus acidilactici viable cell by overcoming lactic acid inhibition

Lactic acid bacteria are industrially important microorganisms recognized for fermentative ability mostly in their probiotic benefits as well as lactic acid production for various applications. Fermentation conditions such as concentration of initial glucose in the culture, concentration of lactic a...

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Main Authors: Othman, Majdiah, Ariff, Arbakariya, Wasoh, Helmi, Kapri, Mohd Rizal, Halim, Murni
Format: Article
Language:English
Published: Springer Open 2017
Online Access:http://psasir.upm.edu.my/id/eprint/63295/1/Strategies%20for%20improving%20production%20performance%20of%20probiotic%20Pediococcus%20acidilactici%20viable%20cell%20by%20overcoming%20lactic%20acid%20inhibition.pdf
http://psasir.upm.edu.my/id/eprint/63295/
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spelling my.upm.eprints.632952018-09-26T01:48:17Z http://psasir.upm.edu.my/id/eprint/63295/ Strategies for improving production performance of probiotic Pediococcus acidilactici viable cell by overcoming lactic acid inhibition Othman, Majdiah Ariff, Arbakariya Wasoh, Helmi Kapri, Mohd Rizal Halim, Murni Lactic acid bacteria are industrially important microorganisms recognized for fermentative ability mostly in their probiotic benefits as well as lactic acid production for various applications. Fermentation conditions such as concentration of initial glucose in the culture, concentration of lactic acid accumulated in the culture, types of pH control strategy, types of aeration mode and different agitation speed had influenced the cultivation performance of batch fermentation of Pediococcus acidilactici. The maximum viable cell concentration obtained in constant fed-batch fermentation at a feeding rate of 0.015 L/h was 6.1 times higher with 1.6 times reduction in lactic acid accumulation compared to batch fermentation. Anion exchange resin, IRA 67 was found to have the highest selectivity towards lactic acid compared to other components studied. Fed-batch fermentation of P. acidilactici coupled with lactic acid removal system using IRA 67 resin showed 55.5 and 9.1 times of improvement in maximum viable cell concentration compared to fermentation without resin for batch and fed-batch mode respectively. The improvement of the P. acidilactici growth in the constant fed-batch fermentation indicated the use of minimal and simple process control equipment is an effective approach for reducing by-product inhibition. Further improvement in the cultivation performance of P. acidilactici in fed-bath fermentation with in situ addition of anion-exchange resin significantly helped to enhance the growth of P. acidilactici by reducing the inhibitory effect of lactic acid and thus increasing probiotic production. Springer Open 2017-12 Article PeerReviewed text en http://psasir.upm.edu.my/id/eprint/63295/1/Strategies%20for%20improving%20production%20performance%20of%20probiotic%20Pediococcus%20acidilactici%20viable%20cell%20by%20overcoming%20lactic%20acid%20inhibition.pdf Othman, Majdiah and Ariff, Arbakariya and Wasoh, Helmi and Kapri, Mohd Rizal and Halim, Murni (2017) Strategies for improving production performance of probiotic Pediococcus acidilactici viable cell by overcoming lactic acid inhibition. AMB Express, 7 (215). pp. 1-14. ISSN 2191-0855 10.1186/s13568-017-0519-6
institution Universiti Putra Malaysia
building UPM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Putra Malaysia
content_source UPM Institutional Repository
url_provider http://psasir.upm.edu.my/
language English
description Lactic acid bacteria are industrially important microorganisms recognized for fermentative ability mostly in their probiotic benefits as well as lactic acid production for various applications. Fermentation conditions such as concentration of initial glucose in the culture, concentration of lactic acid accumulated in the culture, types of pH control strategy, types of aeration mode and different agitation speed had influenced the cultivation performance of batch fermentation of Pediococcus acidilactici. The maximum viable cell concentration obtained in constant fed-batch fermentation at a feeding rate of 0.015 L/h was 6.1 times higher with 1.6 times reduction in lactic acid accumulation compared to batch fermentation. Anion exchange resin, IRA 67 was found to have the highest selectivity towards lactic acid compared to other components studied. Fed-batch fermentation of P. acidilactici coupled with lactic acid removal system using IRA 67 resin showed 55.5 and 9.1 times of improvement in maximum viable cell concentration compared to fermentation without resin for batch and fed-batch mode respectively. The improvement of the P. acidilactici growth in the constant fed-batch fermentation indicated the use of minimal and simple process control equipment is an effective approach for reducing by-product inhibition. Further improvement in the cultivation performance of P. acidilactici in fed-bath fermentation with in situ addition of anion-exchange resin significantly helped to enhance the growth of P. acidilactici by reducing the inhibitory effect of lactic acid and thus increasing probiotic production.
format Article
author Othman, Majdiah
Ariff, Arbakariya
Wasoh, Helmi
Kapri, Mohd Rizal
Halim, Murni
spellingShingle Othman, Majdiah
Ariff, Arbakariya
Wasoh, Helmi
Kapri, Mohd Rizal
Halim, Murni
Strategies for improving production performance of probiotic Pediococcus acidilactici viable cell by overcoming lactic acid inhibition
author_facet Othman, Majdiah
Ariff, Arbakariya
Wasoh, Helmi
Kapri, Mohd Rizal
Halim, Murni
author_sort Othman, Majdiah
title Strategies for improving production performance of probiotic Pediococcus acidilactici viable cell by overcoming lactic acid inhibition
title_short Strategies for improving production performance of probiotic Pediococcus acidilactici viable cell by overcoming lactic acid inhibition
title_full Strategies for improving production performance of probiotic Pediococcus acidilactici viable cell by overcoming lactic acid inhibition
title_fullStr Strategies for improving production performance of probiotic Pediococcus acidilactici viable cell by overcoming lactic acid inhibition
title_full_unstemmed Strategies for improving production performance of probiotic Pediococcus acidilactici viable cell by overcoming lactic acid inhibition
title_sort strategies for improving production performance of probiotic pediococcus acidilactici viable cell by overcoming lactic acid inhibition
publisher Springer Open
publishDate 2017
url http://psasir.upm.edu.my/id/eprint/63295/1/Strategies%20for%20improving%20production%20performance%20of%20probiotic%20Pediococcus%20acidilactici%20viable%20cell%20by%20overcoming%20lactic%20acid%20inhibition.pdf
http://psasir.upm.edu.my/id/eprint/63295/
_version_ 1643837766908248064
score 13.211869

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