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Determination of the presence of huanglongbing in seeds and movement of the pathogen in Citrus reticulata

Problem statement: Huanglongbing (HLB) also known as citrus greening disease is a fastidious phloem-inhabiting bacterium in the genus Candidatus Liberibacter. Using universal primers, the 16S ribosomal DNA sequence of three strains of the bacterium were obtained by PCR. However there is very little...

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Main Authors: Shokrollah, Hajivand, Abdullah, Thohirah Lee, Sijam, Kamaruzaman, Abdullah, Siti Nor Akmar
Format: Article
Language:English
Published: Science Publications 2009
Online Access:http://psasir.upm.edu.my/id/eprint/6319/1/ajassp.2009.1180.1185.pdf
http://psasir.upm.edu.my/id/eprint/6319/
http://thescipub.com/abstract/10.3844/ajassp.2009.1180.1185
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spelling my.upm.eprints.63192017-11-30T08:42:34Z http://psasir.upm.edu.my/id/eprint/6319/ Determination of the presence of huanglongbing in seeds and movement of the pathogen in Citrus reticulata Shokrollah, Hajivand Abdullah, Thohirah Lee Sijam, Kamaruzaman Abdullah, Siti Nor Akmar Problem statement: Huanglongbing (HLB) also known as citrus greening disease is a fastidious phloem-inhabiting bacterium in the genus Candidatus Liberibacter. Using universal primers, the 16S ribosomal DNA sequence of three strains of the bacterium were obtained by PCR. However there is very little information on seed transmission and HLB pathogen movement to find a way for control or reduce the severity of HLB on the field. The study was conducted to detect HLB pathogen in seeds of Citrus, to determine pathogen movement in citrus seedling after infection and to detect the HLB pathogen in citrus roots. Approach: Seeds of Citrus reticulata cv. Limau Madu were collected from infected orchard and were germinated in screenhouse condition. The seeds of Citrus reticlata cv. L. Madu were planted in screenhouse too for HLB pathogen movement and HLB detection in roots. The seedlings were inoculated using infected grafting methods. Results: HLB was not amplified in new seedlings after germination. HLB moved slowly reaching up to 1.5 cm after 2 weeks, 1.5-4.5 cm after eight weeks and detected on 4.5-9 cm after 14 weeks below the grafting area. HLB was also detected up to 9-15 cm after 16 weeks, 15-24 cm after twenty weeks, 24-28.5 cm after 22 weeks and 28.5-30 cm after 24 weeks below the grafting area. Conclusion: Base on conventional PCR test, HLB disease in citrus is not seed borne and it can reach to the roots 26 weeks after inoculation. Science Publications 2009 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/6319/1/ajassp.2009.1180.1185.pdf Shokrollah, Hajivand and Abdullah, Thohirah Lee and Sijam, Kamaruzaman and Abdullah, Siti Nor Akmar (2009) Determination of the presence of huanglongbing in seeds and movement of the pathogen in Citrus reticulata. American Journal of Applied Sciences, 6 (6). pp. 1180-1185. ISSN 1546-9239; ESSN: 1554-3641 http://thescipub.com/abstract/10.3844/ajassp.2009.1180.1185 10.3844/ajassp.2009.1180.1185
institution Universiti Putra Malaysia
building UPM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Putra Malaysia
content_source UPM Institutional Repository
url_provider http://psasir.upm.edu.my/
language English
description Problem statement: Huanglongbing (HLB) also known as citrus greening disease is a fastidious phloem-inhabiting bacterium in the genus Candidatus Liberibacter. Using universal primers, the 16S ribosomal DNA sequence of three strains of the bacterium were obtained by PCR. However there is very little information on seed transmission and HLB pathogen movement to find a way for control or reduce the severity of HLB on the field. The study was conducted to detect HLB pathogen in seeds of Citrus, to determine pathogen movement in citrus seedling after infection and to detect the HLB pathogen in citrus roots. Approach: Seeds of Citrus reticulata cv. Limau Madu were collected from infected orchard and were germinated in screenhouse condition. The seeds of Citrus reticlata cv. L. Madu were planted in screenhouse too for HLB pathogen movement and HLB detection in roots. The seedlings were inoculated using infected grafting methods. Results: HLB was not amplified in new seedlings after germination. HLB moved slowly reaching up to 1.5 cm after 2 weeks, 1.5-4.5 cm after eight weeks and detected on 4.5-9 cm after 14 weeks below the grafting area. HLB was also detected up to 9-15 cm after 16 weeks, 15-24 cm after twenty weeks, 24-28.5 cm after 22 weeks and 28.5-30 cm after 24 weeks below the grafting area. Conclusion: Base on conventional PCR test, HLB disease in citrus is not seed borne and it can reach to the roots 26 weeks after inoculation.
format Article
author Shokrollah, Hajivand
Abdullah, Thohirah Lee
Sijam, Kamaruzaman
Abdullah, Siti Nor Akmar
spellingShingle Shokrollah, Hajivand
Abdullah, Thohirah Lee
Sijam, Kamaruzaman
Abdullah, Siti Nor Akmar
Determination of the presence of huanglongbing in seeds and movement of the pathogen in Citrus reticulata
author_facet Shokrollah, Hajivand
Abdullah, Thohirah Lee
Sijam, Kamaruzaman
Abdullah, Siti Nor Akmar
author_sort Shokrollah, Hajivand
title Determination of the presence of huanglongbing in seeds and movement of the pathogen in Citrus reticulata
title_short Determination of the presence of huanglongbing in seeds and movement of the pathogen in Citrus reticulata
title_full Determination of the presence of huanglongbing in seeds and movement of the pathogen in Citrus reticulata
title_fullStr Determination of the presence of huanglongbing in seeds and movement of the pathogen in Citrus reticulata
title_full_unstemmed Determination of the presence of huanglongbing in seeds and movement of the pathogen in Citrus reticulata
title_sort determination of the presence of huanglongbing in seeds and movement of the pathogen in citrus reticulata
publisher Science Publications
publishDate 2009
url http://psasir.upm.edu.my/id/eprint/6319/1/ajassp.2009.1180.1185.pdf
http://psasir.upm.edu.my/id/eprint/6319/
http://thescipub.com/abstract/10.3844/ajassp.2009.1180.1185
_version_ 1643823452821389312
score 13.211869

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