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Reductive evolution in outer membrane protein biogenesis has not compromised cell surface complexity in Helicobacter pylori

Helicobacter pylori is a gram-negative bacterial pathogen that chronically inhabits the human stomach. To survive and maintain advantage, it has evolved unique host-pathogen interactions mediated by Helicobacter-specific proteins in the bacterial outer membrane. These outer membrane proteins (OMPs)...

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Main Authors: Webb, Chaille T., Chandrapala, Dilini, Oslan, Siti Nurbaya, Bamert, Rebecca S., Grinter, Rhys D., Dunstan, Rhys A., Gorrell, Rebecca J., Song, Jiangning, Strugnell, Richard A., Lithgow, Trevor, Kwok, Terry
Format: Article
Language:English
Published: John Wiley & Sons 2017
Online Access:http://psasir.upm.edu.my/id/eprint/62930/1/Reductive%20evolution%20in%20outer%20membrane%20protein%20biogenesis.pdf
http://psasir.upm.edu.my/id/eprint/62930/
https://onlinelibrary.wiley.com/doi/epdf/10.1002/mbo3.513
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spelling my.upm.eprints.629302018-09-28T10:37:16Z http://psasir.upm.edu.my/id/eprint/62930/ Reductive evolution in outer membrane protein biogenesis has not compromised cell surface complexity in Helicobacter pylori Webb, Chaille T. Chandrapala, Dilini Oslan, Siti Nurbaya Bamert, Rebecca S. Grinter, Rhys D. Dunstan, Rhys A. Gorrell, Rebecca J. Song, Jiangning Strugnell, Richard A. Lithgow, Trevor Kwok, Terry Helicobacter pylori is a gram-negative bacterial pathogen that chronically inhabits the human stomach. To survive and maintain advantage, it has evolved unique host-pathogen interactions mediated by Helicobacter-specific proteins in the bacterial outer membrane. These outer membrane proteins (OMPs) are anchored to the cell surface via a C-terminal β-barrel domain, which requires their assembly by the β-barrel assembly machinery (BAM). Here we have assessed the complexity of the OMP C-terminal β-barrel domains employed by H. pylori, and characterized the H. pyloriBAM complex. Around 50 Helicobacter-specific OMPs were assessed with predictive structural algorithms. The data suggest that H. pylori utilizes a unique β-barrel architecture that might constitute H. pylori-specific Type V secretions system. The structural and functional diversity in these proteins is encompassed by their extramembrane domains. Bioinformatic and biochemical characterization suggests that the low β-barrel-complexity requires only minimalist assembly machinery. The H. pylori proteins BamA and BamD associate to form a BAM complex, with features of BamA enabling an oligomerization that might represent a mechanism by which a minimalist BAM complex forms a larger, sophisticated machinery capable of servicing the outer membrane proteome of H. pylori. John Wiley & Sons 2017-10-21 Article PeerReviewed text en http://psasir.upm.edu.my/id/eprint/62930/1/Reductive%20evolution%20in%20outer%20membrane%20protein%20biogenesis.pdf Webb, Chaille T. and Chandrapala, Dilini and Oslan, Siti Nurbaya and Bamert, Rebecca S. and Grinter, Rhys D. and Dunstan, Rhys A. and Gorrell, Rebecca J. and Song, Jiangning and Strugnell, Richard A. and Lithgow, Trevor and Kwok, Terry (2017) Reductive evolution in outer membrane protein biogenesis has not compromised cell surface complexity in Helicobacter pylori. MicrobiologyOpen, 6 (6). pp. 1-17. ISSN 2045-8827 https://onlinelibrary.wiley.com/doi/epdf/10.1002/mbo3.513 10.1002/mbo3.513
institution Universiti Putra Malaysia
building UPM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Putra Malaysia
content_source UPM Institutional Repository
url_provider http://psasir.upm.edu.my/
language English
description Helicobacter pylori is a gram-negative bacterial pathogen that chronically inhabits the human stomach. To survive and maintain advantage, it has evolved unique host-pathogen interactions mediated by Helicobacter-specific proteins in the bacterial outer membrane. These outer membrane proteins (OMPs) are anchored to the cell surface via a C-terminal β-barrel domain, which requires their assembly by the β-barrel assembly machinery (BAM). Here we have assessed the complexity of the OMP C-terminal β-barrel domains employed by H. pylori, and characterized the H. pyloriBAM complex. Around 50 Helicobacter-specific OMPs were assessed with predictive structural algorithms. The data suggest that H. pylori utilizes a unique β-barrel architecture that might constitute H. pylori-specific Type V secretions system. The structural and functional diversity in these proteins is encompassed by their extramembrane domains. Bioinformatic and biochemical characterization suggests that the low β-barrel-complexity requires only minimalist assembly machinery. The H. pylori proteins BamA and BamD associate to form a BAM complex, with features of BamA enabling an oligomerization that might represent a mechanism by which a minimalist BAM complex forms a larger, sophisticated machinery capable of servicing the outer membrane proteome of H. pylori.
format Article
author Webb, Chaille T.
Chandrapala, Dilini
Oslan, Siti Nurbaya
Bamert, Rebecca S.
Grinter, Rhys D.
Dunstan, Rhys A.
Gorrell, Rebecca J.
Song, Jiangning
Strugnell, Richard A.
Lithgow, Trevor
Kwok, Terry
spellingShingle Webb, Chaille T.
Chandrapala, Dilini
Oslan, Siti Nurbaya
Bamert, Rebecca S.
Grinter, Rhys D.
Dunstan, Rhys A.
Gorrell, Rebecca J.
Song, Jiangning
Strugnell, Richard A.
Lithgow, Trevor
Kwok, Terry
Reductive evolution in outer membrane protein biogenesis has not compromised cell surface complexity in Helicobacter pylori
author_facet Webb, Chaille T.
Chandrapala, Dilini
Oslan, Siti Nurbaya
Bamert, Rebecca S.
Grinter, Rhys D.
Dunstan, Rhys A.
Gorrell, Rebecca J.
Song, Jiangning
Strugnell, Richard A.
Lithgow, Trevor
Kwok, Terry
author_sort Webb, Chaille T.
title Reductive evolution in outer membrane protein biogenesis has not compromised cell surface complexity in Helicobacter pylori
title_short Reductive evolution in outer membrane protein biogenesis has not compromised cell surface complexity in Helicobacter pylori
title_full Reductive evolution in outer membrane protein biogenesis has not compromised cell surface complexity in Helicobacter pylori
title_fullStr Reductive evolution in outer membrane protein biogenesis has not compromised cell surface complexity in Helicobacter pylori
title_full_unstemmed Reductive evolution in outer membrane protein biogenesis has not compromised cell surface complexity in Helicobacter pylori
title_sort reductive evolution in outer membrane protein biogenesis has not compromised cell surface complexity in helicobacter pylori
publisher John Wiley & Sons
publishDate 2017
url http://psasir.upm.edu.my/id/eprint/62930/1/Reductive%20evolution%20in%20outer%20membrane%20protein%20biogenesis.pdf
http://psasir.upm.edu.my/id/eprint/62930/
https://onlinelibrary.wiley.com/doi/epdf/10.1002/mbo3.513
_version_ 1643837712686383104
score 13.211869

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