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First report of methicillin-resistant Staphylococcus aureus from cage-cultured tilapia (Oreochromis niloticus)

Swabs from the brain, eyes and kidneys of tilapia from 11 farms were collected for a period of 2 years. They were grown on blood agar before cultures of suspected Staphylococcus aureus were subjected to ABI STAPH Detection Kit and PCR for identification. They were then grown on oxacillin resistance...

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Main Authors: M. A. S., Atyah, Saad, Mohd Zamri, Abdullah, Siti Zahrah
Format: Article
Language:English
English
Published: Elsevier 2010
Online Access:http://psasir.upm.edu.my/id/eprint/6285/1/First%20report%20of%20methicillin.pdf
http://psasir.upm.edu.my/id/eprint/6285/
http://dx.doi.org/10.1016/j.vetmic.2010.02.004
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spelling my.upm.eprints.62852015-10-20T08:58:02Z http://psasir.upm.edu.my/id/eprint/6285/ First report of methicillin-resistant Staphylococcus aureus from cage-cultured tilapia (Oreochromis niloticus) M. A. S., Atyah Saad, Mohd Zamri Abdullah, Siti Zahrah Swabs from the brain, eyes and kidneys of tilapia from 11 farms were collected for a period of 2 years. They were grown on blood agar before cultures of suspected Staphylococcus aureus were subjected to ABI STAPH Detection Kit and PCR for identification. They were then grown on oxacillin resistance screening agar base (ORSAB) and subjected to PCR using the MRSA 17 kb forward and reverse primers to identify the methicillin-resistant S. aureus (MRSA). A total of 559 isolates of Staphylococcus spp. were obtained, from which 198 (35%)isolates were identified as S. aureus. Of the 198 S. aureus isolated from tilapias, 98 (50%) were identified as methicillin-resistant S. aureus (MRSA). Since global spread of multidrug-resistant bacteria has increased in the past decade, this new finding in fish should be of concern. Elsevier 2010-02-03 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/6285/1/First%20report%20of%20methicillin.pdf M. A. S., Atyah and Saad, Mohd Zamri and Abdullah, Siti Zahrah (2010) First report of methicillin-resistant Staphylococcus aureus from cage-cultured tilapia (Oreochromis niloticus). Veterinary Microbiology, 144 (3-4). pp. 502-504. ISSN 0378-1135 http://dx.doi.org/10.1016/j.vetmic.2010.02.004 10.1016/j.vetmic.2010.02.004 English
institution Universiti Putra Malaysia
building UPM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Putra Malaysia
content_source UPM Institutional Repository
url_provider http://psasir.upm.edu.my/
language English
English
description Swabs from the brain, eyes and kidneys of tilapia from 11 farms were collected for a period of 2 years. They were grown on blood agar before cultures of suspected Staphylococcus aureus were subjected to ABI STAPH Detection Kit and PCR for identification. They were then grown on oxacillin resistance screening agar base (ORSAB) and subjected to PCR using the MRSA 17 kb forward and reverse primers to identify the methicillin-resistant S. aureus (MRSA). A total of 559 isolates of Staphylococcus spp. were obtained, from which 198 (35%)isolates were identified as S. aureus. Of the 198 S. aureus isolated from tilapias, 98 (50%) were identified as methicillin-resistant S. aureus (MRSA). Since global spread of multidrug-resistant bacteria has increased in the past decade, this new finding in fish should be of concern.
format Article
author M. A. S., Atyah
Saad, Mohd Zamri
Abdullah, Siti Zahrah
spellingShingle M. A. S., Atyah
Saad, Mohd Zamri
Abdullah, Siti Zahrah
First report of methicillin-resistant Staphylococcus aureus from cage-cultured tilapia (Oreochromis niloticus)
author_facet M. A. S., Atyah
Saad, Mohd Zamri
Abdullah, Siti Zahrah
author_sort M. A. S., Atyah
title First report of methicillin-resistant Staphylococcus aureus from cage-cultured tilapia (Oreochromis niloticus)
title_short First report of methicillin-resistant Staphylococcus aureus from cage-cultured tilapia (Oreochromis niloticus)
title_full First report of methicillin-resistant Staphylococcus aureus from cage-cultured tilapia (Oreochromis niloticus)
title_fullStr First report of methicillin-resistant Staphylococcus aureus from cage-cultured tilapia (Oreochromis niloticus)
title_full_unstemmed First report of methicillin-resistant Staphylococcus aureus from cage-cultured tilapia (Oreochromis niloticus)
title_sort first report of methicillin-resistant staphylococcus aureus from cage-cultured tilapia (oreochromis niloticus)
publisher Elsevier
publishDate 2010
url http://psasir.upm.edu.my/id/eprint/6285/1/First%20report%20of%20methicillin.pdf
http://psasir.upm.edu.my/id/eprint/6285/
http://dx.doi.org/10.1016/j.vetmic.2010.02.004
_version_ 1643823442387009536
score 13.211869

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