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Prevalence and antibiotic resistance against tetracycline in Campylobacter jejuni and C. coli in cattle and beef meat from Selangor, Malaysia

Campylobacter is a major foodborne pathogen frequently associated with human bacterial gastroenteritis in the world. This study was conducted to determine the prevalence and antibiotic resistance of Campylobacter spp. in the beef food system in Malaysia. A total of 340 samples consisting of cattle f...

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Main Authors: Premarathne, Jayasekara M. K. J. K., Anuar, Aimi Syazana, Thung, Tze Young, Satharasinghe, Dilan Amila, Jambari, Nuzul Noorahya, Abdul Mutalib, Noor Azira, Tang, John Yew Huat, Basri, Dayang Fredalina, Rukayadi, Yaya, Nakaguchi, Yoshitsugu, Nishibuchi, Mitsuaki, Radu, Son
Format: Article
Language:English
Published: Frontiers Research Foundation 2017
Online Access:http://psasir.upm.edu.my/id/eprint/62838/1/Prevalence%20and%20antibiotic%20resistance%20.pdf
http://psasir.upm.edu.my/id/eprint/62838/
https://www.frontiersin.org/articles/10.3389/fmicb.2017.02254/full
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spelling my.upm.eprints.628382022-11-21T00:16:17Z http://psasir.upm.edu.my/id/eprint/62838/ Prevalence and antibiotic resistance against tetracycline in Campylobacter jejuni and C. coli in cattle and beef meat from Selangor, Malaysia Premarathne, Jayasekara M. K. J. K. Anuar, Aimi Syazana Thung, Tze Young Satharasinghe, Dilan Amila Jambari, Nuzul Noorahya Abdul Mutalib, Noor Azira Tang, John Yew Huat Basri, Dayang Fredalina Rukayadi, Yaya Nakaguchi, Yoshitsugu Nishibuchi, Mitsuaki Radu, Son Campylobacter is a major foodborne pathogen frequently associated with human bacterial gastroenteritis in the world. This study was conducted to determine the prevalence and antibiotic resistance of Campylobacter spp. in the beef food system in Malaysia. A total of 340 samples consisting of cattle feces (n = 100), beef (n = 120) from wet markets and beef (n = 120) from hypermarkets were analyzed for Campylobacter spp. The overall prevalence of Campylobacter was 17.4%, consisting of 33% in cattle fecal samples, 14.2% in raw beef from wet market and 7.5% in raw beef from the hypermarket. The multiplex-polymerase chain reaction (PCR) identified 55% of the strains as C. jejuni, 26% as C. coli, and 19% as other Campylobacter spp. A high percentage of Campylobacter spp. were resistant to tetracycline (76.9%) and ampicillin (69.2%), whilst low resistance was exhibited to chloramphenicol (7.6%). The MAR Index of Campylobacter isolates from this study ranged from 0.09 to 0.73. The present study indicates the potential public health risk associated with the beef food system, hence stringent surveillance, regulatory measures, and appropriate interventions are required to minimize Campylobacter contamination and prudent antibiotic usage that can ensure consumer safety. Frontiers Research Foundation 2017-12 Article PeerReviewed text en http://psasir.upm.edu.my/id/eprint/62838/1/Prevalence%20and%20antibiotic%20resistance%20.pdf Premarathne, Jayasekara M. K. J. K. and Anuar, Aimi Syazana and Thung, Tze Young and Satharasinghe, Dilan Amila and Jambari, Nuzul Noorahya and Abdul Mutalib, Noor Azira and Tang, John Yew Huat and Basri, Dayang Fredalina and Rukayadi, Yaya and Nakaguchi, Yoshitsugu and Nishibuchi, Mitsuaki and Radu, Son (2017) Prevalence and antibiotic resistance against tetracycline in Campylobacter jejuni and C. coli in cattle and beef meat from Selangor, Malaysia. Frontiers in Microbiology, 8. art. no. 2254. pp. 1-9. ISSN 1664-302X https://www.frontiersin.org/articles/10.3389/fmicb.2017.02254/full 10.3389/fmicb.2017.02254
institution Universiti Putra Malaysia
building UPM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Putra Malaysia
content_source UPM Institutional Repository
url_provider http://psasir.upm.edu.my/
language English
description Campylobacter is a major foodborne pathogen frequently associated with human bacterial gastroenteritis in the world. This study was conducted to determine the prevalence and antibiotic resistance of Campylobacter spp. in the beef food system in Malaysia. A total of 340 samples consisting of cattle feces (n = 100), beef (n = 120) from wet markets and beef (n = 120) from hypermarkets were analyzed for Campylobacter spp. The overall prevalence of Campylobacter was 17.4%, consisting of 33% in cattle fecal samples, 14.2% in raw beef from wet market and 7.5% in raw beef from the hypermarket. The multiplex-polymerase chain reaction (PCR) identified 55% of the strains as C. jejuni, 26% as C. coli, and 19% as other Campylobacter spp. A high percentage of Campylobacter spp. were resistant to tetracycline (76.9%) and ampicillin (69.2%), whilst low resistance was exhibited to chloramphenicol (7.6%). The MAR Index of Campylobacter isolates from this study ranged from 0.09 to 0.73. The present study indicates the potential public health risk associated with the beef food system, hence stringent surveillance, regulatory measures, and appropriate interventions are required to minimize Campylobacter contamination and prudent antibiotic usage that can ensure consumer safety.
format Article
author Premarathne, Jayasekara M. K. J. K.
Anuar, Aimi Syazana
Thung, Tze Young
Satharasinghe, Dilan Amila
Jambari, Nuzul Noorahya
Abdul Mutalib, Noor Azira
Tang, John Yew Huat
Basri, Dayang Fredalina
Rukayadi, Yaya
Nakaguchi, Yoshitsugu
Nishibuchi, Mitsuaki
Radu, Son
spellingShingle Premarathne, Jayasekara M. K. J. K.
Anuar, Aimi Syazana
Thung, Tze Young
Satharasinghe, Dilan Amila
Jambari, Nuzul Noorahya
Abdul Mutalib, Noor Azira
Tang, John Yew Huat
Basri, Dayang Fredalina
Rukayadi, Yaya
Nakaguchi, Yoshitsugu
Nishibuchi, Mitsuaki
Radu, Son
Prevalence and antibiotic resistance against tetracycline in Campylobacter jejuni and C. coli in cattle and beef meat from Selangor, Malaysia
author_facet Premarathne, Jayasekara M. K. J. K.
Anuar, Aimi Syazana
Thung, Tze Young
Satharasinghe, Dilan Amila
Jambari, Nuzul Noorahya
Abdul Mutalib, Noor Azira
Tang, John Yew Huat
Basri, Dayang Fredalina
Rukayadi, Yaya
Nakaguchi, Yoshitsugu
Nishibuchi, Mitsuaki
Radu, Son
author_sort Premarathne, Jayasekara M. K. J. K.
title Prevalence and antibiotic resistance against tetracycline in Campylobacter jejuni and C. coli in cattle and beef meat from Selangor, Malaysia
title_short Prevalence and antibiotic resistance against tetracycline in Campylobacter jejuni and C. coli in cattle and beef meat from Selangor, Malaysia
title_full Prevalence and antibiotic resistance against tetracycline in Campylobacter jejuni and C. coli in cattle and beef meat from Selangor, Malaysia
title_fullStr Prevalence and antibiotic resistance against tetracycline in Campylobacter jejuni and C. coli in cattle and beef meat from Selangor, Malaysia
title_full_unstemmed Prevalence and antibiotic resistance against tetracycline in Campylobacter jejuni and C. coli in cattle and beef meat from Selangor, Malaysia
title_sort prevalence and antibiotic resistance against tetracycline in campylobacter jejuni and c. coli in cattle and beef meat from selangor, malaysia
publisher Frontiers Research Foundation
publishDate 2017
url http://psasir.upm.edu.my/id/eprint/62838/1/Prevalence%20and%20antibiotic%20resistance%20.pdf
http://psasir.upm.edu.my/id/eprint/62838/
https://www.frontiersin.org/articles/10.3389/fmicb.2017.02254/full
_version_ 1751538186524295168
score 13.211869

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