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Polymeric design of cell culture materials that guide the differentiation of human pluripotent stem cells

Human pluripotent stem cells (hPSCs), including embryonic stem cells (hESCs) and induced pluripotent stem cells (hiPSCs), have the potential to differentiate into many cell types that originate from the three germ layers, such as dopamine-secreting cells and insulin-secreting cells for the treatment...

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Main Authors: Higuchi, Akon, Subbiah, Suresh Kumar, Ling, Qing-Dong, A. Alarfaj, Abdullah, Munusamy, Murugan A., Murugan, Kadarkarai, Hsu, Shih-Tien, Benelli, Giovanni, Umezawa, Akihiro
Format: Article
Language:English
Published: Elsevier 2017
Online Access:http://psasir.upm.edu.my/id/eprint/62779/1/Polymeric%20design%20of%20cell%20.pdf
http://psasir.upm.edu.my/id/eprint/62779/
https://reader.elsevier.com/reader/sd/pii/S007967001630065X?token=59ECB83AC389531DB3F3256CB96A7493C4C0C37826285ADECE0C39DA8C03118FE1AC67A1859639D7E38CBA878D968354&originRegion=eu-west-1&originCreation=20220921073042
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spelling my.upm.eprints.627792022-11-08T07:46:10Z http://psasir.upm.edu.my/id/eprint/62779/ Polymeric design of cell culture materials that guide the differentiation of human pluripotent stem cells Higuchi, Akon Subbiah, Suresh Kumar Ling, Qing-Dong A. Alarfaj, Abdullah Munusamy, Murugan A. Murugan, Kadarkarai Hsu, Shih-Tien Benelli, Giovanni Umezawa, Akihiro Human pluripotent stem cells (hPSCs), including embryonic stem cells (hESCs) and induced pluripotent stem cells (hiPSCs), have the potential to differentiate into many cell types that originate from the three germ layers, such as dopamine-secreting cells and insulin-secreting cells for the treatment of Alzheimer's disease and diabetes, respectively. However, it is challenging to guide hPSC differentiation into desired cell lineages due to their varying differentiation ability. A reasonable strategy is to mimic the stem cell microenvironment for the differentiation of hPSCs into specific cell lineages using optimal polymeric biomaterials for hPSC culture. This review summarizes various methods for differentiating hPSCs cultured on polymeric biomaterials and discusses the optimal methods and cell culture polymeric biomaterials for hPSC differentiation into specific cell lineages. The recent trend in protocols avoids embryoid body (EB, aggregated cells) formation because EBs contain different types of cells. The combination of appropriate differentiation protocols and cell culture polymeric biomaterials for the differentiation of hPSCs into specific cell lineages will produce a large quantity of highly pure GMP-grade differentiated cells for use in translational medicine. Elsevier 2017-02 Article PeerReviewed text en http://psasir.upm.edu.my/id/eprint/62779/1/Polymeric%20design%20of%20cell%20.pdf Higuchi, Akon and Subbiah, Suresh Kumar and Ling, Qing-Dong and A. Alarfaj, Abdullah and Munusamy, Murugan A. and Murugan, Kadarkarai and Hsu, Shih-Tien and Benelli, Giovanni and Umezawa, Akihiro (2017) Polymeric design of cell culture materials that guide the differentiation of human pluripotent stem cells. Progress in Polymer Science, 65. 83 - 126. ISSN 0079-6700 https://reader.elsevier.com/reader/sd/pii/S007967001630065X?token=59ECB83AC389531DB3F3256CB96A7493C4C0C37826285ADECE0C39DA8C03118FE1AC67A1859639D7E38CBA878D968354&originRegion=eu-west-1&originCreation=20220921073042 10.1016/j.progpolymsci.2016.09.002
institution Universiti Putra Malaysia
building UPM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Putra Malaysia
content_source UPM Institutional Repository
url_provider http://psasir.upm.edu.my/
language English
description Human pluripotent stem cells (hPSCs), including embryonic stem cells (hESCs) and induced pluripotent stem cells (hiPSCs), have the potential to differentiate into many cell types that originate from the three germ layers, such as dopamine-secreting cells and insulin-secreting cells for the treatment of Alzheimer's disease and diabetes, respectively. However, it is challenging to guide hPSC differentiation into desired cell lineages due to their varying differentiation ability. A reasonable strategy is to mimic the stem cell microenvironment for the differentiation of hPSCs into specific cell lineages using optimal polymeric biomaterials for hPSC culture. This review summarizes various methods for differentiating hPSCs cultured on polymeric biomaterials and discusses the optimal methods and cell culture polymeric biomaterials for hPSC differentiation into specific cell lineages. The recent trend in protocols avoids embryoid body (EB, aggregated cells) formation because EBs contain different types of cells. The combination of appropriate differentiation protocols and cell culture polymeric biomaterials for the differentiation of hPSCs into specific cell lineages will produce a large quantity of highly pure GMP-grade differentiated cells for use in translational medicine.
format Article
author Higuchi, Akon
Subbiah, Suresh Kumar
Ling, Qing-Dong
A. Alarfaj, Abdullah
Munusamy, Murugan A.
Murugan, Kadarkarai
Hsu, Shih-Tien
Benelli, Giovanni
Umezawa, Akihiro
spellingShingle Higuchi, Akon
Subbiah, Suresh Kumar
Ling, Qing-Dong
A. Alarfaj, Abdullah
Munusamy, Murugan A.
Murugan, Kadarkarai
Hsu, Shih-Tien
Benelli, Giovanni
Umezawa, Akihiro
Polymeric design of cell culture materials that guide the differentiation of human pluripotent stem cells
author_facet Higuchi, Akon
Subbiah, Suresh Kumar
Ling, Qing-Dong
A. Alarfaj, Abdullah
Munusamy, Murugan A.
Murugan, Kadarkarai
Hsu, Shih-Tien
Benelli, Giovanni
Umezawa, Akihiro
author_sort Higuchi, Akon
title Polymeric design of cell culture materials that guide the differentiation of human pluripotent stem cells
title_short Polymeric design of cell culture materials that guide the differentiation of human pluripotent stem cells
title_full Polymeric design of cell culture materials that guide the differentiation of human pluripotent stem cells
title_fullStr Polymeric design of cell culture materials that guide the differentiation of human pluripotent stem cells
title_full_unstemmed Polymeric design of cell culture materials that guide the differentiation of human pluripotent stem cells
title_sort polymeric design of cell culture materials that guide the differentiation of human pluripotent stem cells
publisher Elsevier
publishDate 2017
url http://psasir.upm.edu.my/id/eprint/62779/1/Polymeric%20design%20of%20cell%20.pdf
http://psasir.upm.edu.my/id/eprint/62779/
https://reader.elsevier.com/reader/sd/pii/S007967001630065X?token=59ECB83AC389531DB3F3256CB96A7493C4C0C37826285ADECE0C39DA8C03118FE1AC67A1859639D7E38CBA878D968354&originRegion=eu-west-1&originCreation=20220921073042
_version_ 1751538180087087104
score 13.211869

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