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Kaempferol and chrysin synergies to improve septic mice survival

Previously, we reported the role of synergy between two flavonoids—namely, chrysin and kaempferol—in inhibiting the secretion of a few major proinflammatory mediators such as tumor necrosis factor-alpha (TNF-α), prostaglandin E2 (PGE2), and nitric oxide (NO) from lipopolysaccharide (LPS)-induced RAW...

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Main Authors: Harasstani, Omar A., Tham, Chau Ling, Israf Ali, Daud Ahmad
Format: Article
Language:English
Published: MDPI 2017
Online Access:http://psasir.upm.edu.my/id/eprint/62248/1/Kaempferol%20and%20chrysin%20synergies%20to%20improve%20septic%20mice%20survival.pdf
http://psasir.upm.edu.my/id/eprint/62248/
https://www.mdpi.com/1420-3049/22/1/92
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spelling my.upm.eprints.622482019-05-23T02:05:50Z http://psasir.upm.edu.my/id/eprint/62248/ Kaempferol and chrysin synergies to improve septic mice survival Harasstani, Omar A. Tham, Chau Ling Israf Ali, Daud Ahmad Previously, we reported the role of synergy between two flavonoids—namely, chrysin and kaempferol—in inhibiting the secretion of a few major proinflammatory mediators such as tumor necrosis factor-alpha (TNF-α), prostaglandin E2 (PGE2), and nitric oxide (NO) from lipopolysaccharide (LPS)-induced RAW 264.7 cells. The present study aims to evaluate the effects of this combination on a murine model of polymicrobial sepsis induced by cecal ligation and puncture (CLP). Severe sepsis was induced in male ICR mice (n = 7) via the CLP procedure. The effects of chrysin and kaempferol combination treatment on septic mice were investigated using a 7-day survival study. The levels of key proinflammatory mediators and markers—such as aspartate aminotransferase (AST), TNF-α, and NO—in the sera samples of the septic mice were determined via ELISA and fluorescence determination at different time point intervals post-CLP challenge. Liver tissue samples from septic mice were harvested to measure myeloperoxidase (MPO) levels using a spectrophotometer. Moreover, intraperitoneal fluid (IPF) bacterial clearance and total leukocyte count were also assessed to detect any antibacterial effects exerted by chrysin and kaempferol, individually and in combination. Kaempferol treatment improved the survival rate of CLP-challenged mice by up to 16%. During this treatment, kaempferol expressed antibacterial, antiapoptotic and antioxidant activities through the attenuation of bacterial forming units, AST and NO levels, and increased polymorphonuclear leukocyte (PMN) count in the IPF. On the other hand, the chrysin treatment significantly reduced serum TNF-α levels. However, it failed to significantly improve the survival rate of the CLP-challenged mice. Subsequently, the kaempferol/chrysin combination treatment significantly improved the overall 7-day survival rate by 2-fold—up to 29%. Kaempferol and chrysin revealed some synergistic effects by acting individually upon multiple pathophysiological factors involved during sepsis. Although the kaempferol/chrysin combination did not exhibit significant antibacterial effects, it did exhibit anti-inflammatory and antioxidant activities, which translate to significant improvement in the survival rate of septic animals. These findings suggest the potential application of this combination treatment as a beneficial adjuvant supplement strategy in sepsis control. MDPI 2017 Article PeerReviewed text en http://psasir.upm.edu.my/id/eprint/62248/1/Kaempferol%20and%20chrysin%20synergies%20to%20improve%20septic%20mice%20survival.pdf Harasstani, Omar A. and Tham, Chau Ling and Israf Ali, Daud Ahmad (2017) Kaempferol and chrysin synergies to improve septic mice survival. Molecules, 22 (1). pp. 1-13. ISSN 1420-3049 https://www.mdpi.com/1420-3049/22/1/92 10.3390/molecules22010092
institution Universiti Putra Malaysia
building UPM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Putra Malaysia
content_source UPM Institutional Repository
url_provider http://psasir.upm.edu.my/
language English
description Previously, we reported the role of synergy between two flavonoids—namely, chrysin and kaempferol—in inhibiting the secretion of a few major proinflammatory mediators such as tumor necrosis factor-alpha (TNF-α), prostaglandin E2 (PGE2), and nitric oxide (NO) from lipopolysaccharide (LPS)-induced RAW 264.7 cells. The present study aims to evaluate the effects of this combination on a murine model of polymicrobial sepsis induced by cecal ligation and puncture (CLP). Severe sepsis was induced in male ICR mice (n = 7) via the CLP procedure. The effects of chrysin and kaempferol combination treatment on septic mice were investigated using a 7-day survival study. The levels of key proinflammatory mediators and markers—such as aspartate aminotransferase (AST), TNF-α, and NO—in the sera samples of the septic mice were determined via ELISA and fluorescence determination at different time point intervals post-CLP challenge. Liver tissue samples from septic mice were harvested to measure myeloperoxidase (MPO) levels using a spectrophotometer. Moreover, intraperitoneal fluid (IPF) bacterial clearance and total leukocyte count were also assessed to detect any antibacterial effects exerted by chrysin and kaempferol, individually and in combination. Kaempferol treatment improved the survival rate of CLP-challenged mice by up to 16%. During this treatment, kaempferol expressed antibacterial, antiapoptotic and antioxidant activities through the attenuation of bacterial forming units, AST and NO levels, and increased polymorphonuclear leukocyte (PMN) count in the IPF. On the other hand, the chrysin treatment significantly reduced serum TNF-α levels. However, it failed to significantly improve the survival rate of the CLP-challenged mice. Subsequently, the kaempferol/chrysin combination treatment significantly improved the overall 7-day survival rate by 2-fold—up to 29%. Kaempferol and chrysin revealed some synergistic effects by acting individually upon multiple pathophysiological factors involved during sepsis. Although the kaempferol/chrysin combination did not exhibit significant antibacterial effects, it did exhibit anti-inflammatory and antioxidant activities, which translate to significant improvement in the survival rate of septic animals. These findings suggest the potential application of this combination treatment as a beneficial adjuvant supplement strategy in sepsis control.
format Article
author Harasstani, Omar A.
Tham, Chau Ling
Israf Ali, Daud Ahmad
spellingShingle Harasstani, Omar A.
Tham, Chau Ling
Israf Ali, Daud Ahmad
Kaempferol and chrysin synergies to improve septic mice survival
author_facet Harasstani, Omar A.
Tham, Chau Ling
Israf Ali, Daud Ahmad
author_sort Harasstani, Omar A.
title Kaempferol and chrysin synergies to improve septic mice survival
title_short Kaempferol and chrysin synergies to improve septic mice survival
title_full Kaempferol and chrysin synergies to improve septic mice survival
title_fullStr Kaempferol and chrysin synergies to improve septic mice survival
title_full_unstemmed Kaempferol and chrysin synergies to improve septic mice survival
title_sort kaempferol and chrysin synergies to improve septic mice survival
publisher MDPI
publishDate 2017
url http://psasir.upm.edu.my/id/eprint/62248/1/Kaempferol%20and%20chrysin%20synergies%20to%20improve%20septic%20mice%20survival.pdf
http://psasir.upm.edu.my/id/eprint/62248/
https://www.mdpi.com/1420-3049/22/1/92
_version_ 1643837704631222272
score 13.211869

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