Cover Image

Isolation of molybdenum-reducing bacterium; Serratia sp. strain MIE2 from agriculture soil and its potential use in soil bioremediation

Molybdenum is reported to be very toxic to ruminants and shows evidence of spermatogenesistoxicity in animals and insects. Hence, its removal is important. In this study, we report on thefirst isolation of molybdenum-reducing bacterium from agricultural soil. The bacterium reduceshexavalent molybden...

Full description

Saved in:
Bibliographic Details
Main Authors: Halmi, Mohd Izuan Effendi, Wan Johari, Wan Lutfi, Mohd Ali, Mohd Shukuri, Shaharuddin, Noor Azmi
Format: Article
Language:English
Published: Hibiscus Publisher 2017
Online Access:http://psasir.upm.edu.my/id/eprint/62240/1/Isolation%20of%20molybdenum-reducing%20bacterium.pdf
http://psasir.upm.edu.my/id/eprint/62240/
https://journal.hibiscuspublisher.com/index.php/JOBIMB/article/view/342
Tags: Add Tag
No Tags, Be the first to tag this record!
id my.upm.eprints.62240
record_format eprints
spelling my.upm.eprints.622402019-05-21T06:08:45Z http://psasir.upm.edu.my/id/eprint/62240/ Isolation of molybdenum-reducing bacterium; Serratia sp. strain MIE2 from agriculture soil and its potential use in soil bioremediation Halmi, Mohd Izuan Effendi Wan Johari, Wan Lutfi Mohd Ali, Mohd Shukuri Shaharuddin, Noor Azmi Molybdenum is reported to be very toxic to ruminants and shows evidence of spermatogenesistoxicity in animals and insects. Hence, its removal is important. In this study, we report on thefirst isolation of molybdenum-reducing bacterium from agricultural soil. The bacterium reduceshexavalent molybdenum (sodium molybdate) to molybdenum blue (Mo-blue); a colloidalproduct, which can be trapped and removed from solution. Phylogenetic analysis resulted in atentative identification of the bacterium as Serratia sp. strain MIE2. The optimum conditions forMo-blue production using the normal one-variable-at-a-time (OVAT) approach were 10 mM ofsodium molybdate, pH 6.0, a temperature of 35°C, ammonium sulphate at 10 g/L as the nitrogensource and sucrose concentrations of between 30-50 g/L as the carbon source and electron donorfor molybdate. Studies on the effects of pesticides and solvents on Mo-blue production showedthat Mo-blue production from whole cells was relatively more affected by these xenobioticscompared to the crude enzyme. Nevertheless, the strain was resistant to most of the xenobioticstested. Based on the strain MIE characteristics, the bacterium will be a suitable candidate for theremediation of aquatic bodies and agricultural soils contaminated with molybdenum. Hibiscus Publisher 2017 Article PeerReviewed text en http://psasir.upm.edu.my/id/eprint/62240/1/Isolation%20of%20molybdenum-reducing%20bacterium.pdf Halmi, Mohd Izuan Effendi and Wan Johari, Wan Lutfi and Mohd Ali, Mohd Shukuri and Shaharuddin, Noor Azmi (2017) Isolation of molybdenum-reducing bacterium; Serratia sp. strain MIE2 from agriculture soil and its potential use in soil bioremediation. Journal of Biochemistry, Microbiology and Biotechnology, 5 (2). pp. 12-18. ISSN 2289-5779 https://journal.hibiscuspublisher.com/index.php/JOBIMB/article/view/342
institution Universiti Putra Malaysia
building UPM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Putra Malaysia
content_source UPM Institutional Repository
url_provider http://psasir.upm.edu.my/
language English
description Molybdenum is reported to be very toxic to ruminants and shows evidence of spermatogenesistoxicity in animals and insects. Hence, its removal is important. In this study, we report on thefirst isolation of molybdenum-reducing bacterium from agricultural soil. The bacterium reduceshexavalent molybdenum (sodium molybdate) to molybdenum blue (Mo-blue); a colloidalproduct, which can be trapped and removed from solution. Phylogenetic analysis resulted in atentative identification of the bacterium as Serratia sp. strain MIE2. The optimum conditions forMo-blue production using the normal one-variable-at-a-time (OVAT) approach were 10 mM ofsodium molybdate, pH 6.0, a temperature of 35°C, ammonium sulphate at 10 g/L as the nitrogensource and sucrose concentrations of between 30-50 g/L as the carbon source and electron donorfor molybdate. Studies on the effects of pesticides and solvents on Mo-blue production showedthat Mo-blue production from whole cells was relatively more affected by these xenobioticscompared to the crude enzyme. Nevertheless, the strain was resistant to most of the xenobioticstested. Based on the strain MIE characteristics, the bacterium will be a suitable candidate for theremediation of aquatic bodies and agricultural soils contaminated with molybdenum.
format Article
author Halmi, Mohd Izuan Effendi
Wan Johari, Wan Lutfi
Mohd Ali, Mohd Shukuri
Shaharuddin, Noor Azmi
spellingShingle Halmi, Mohd Izuan Effendi
Wan Johari, Wan Lutfi
Mohd Ali, Mohd Shukuri
Shaharuddin, Noor Azmi
Isolation of molybdenum-reducing bacterium; Serratia sp. strain MIE2 from agriculture soil and its potential use in soil bioremediation
author_facet Halmi, Mohd Izuan Effendi
Wan Johari, Wan Lutfi
Mohd Ali, Mohd Shukuri
Shaharuddin, Noor Azmi
author_sort Halmi, Mohd Izuan Effendi
title Isolation of molybdenum-reducing bacterium; Serratia sp. strain MIE2 from agriculture soil and its potential use in soil bioremediation
title_short Isolation of molybdenum-reducing bacterium; Serratia sp. strain MIE2 from agriculture soil and its potential use in soil bioremediation
title_full Isolation of molybdenum-reducing bacterium; Serratia sp. strain MIE2 from agriculture soil and its potential use in soil bioremediation
title_fullStr Isolation of molybdenum-reducing bacterium; Serratia sp. strain MIE2 from agriculture soil and its potential use in soil bioremediation
title_full_unstemmed Isolation of molybdenum-reducing bacterium; Serratia sp. strain MIE2 from agriculture soil and its potential use in soil bioremediation
title_sort isolation of molybdenum-reducing bacterium; serratia sp. strain mie2 from agriculture soil and its potential use in soil bioremediation
publisher Hibiscus Publisher
publishDate 2017
url http://psasir.upm.edu.my/id/eprint/62240/1/Isolation%20of%20molybdenum-reducing%20bacterium.pdf
http://psasir.upm.edu.my/id/eprint/62240/
https://journal.hibiscuspublisher.com/index.php/JOBIMB/article/view/342
_version_ 1643837703154827264
score 13.211869

Similar Items