Cover Image

Isolation and identification of bacterial populations of zoonotic importance from captive non-venomous snakes in Malaysia

Aim: Captivity of non-venomous snakes such as python and boa are common in zoos, aquariums and as pets in households. Poor captivity conditions expose these reptiles to numerous pathogens which may result in disease conditions. The purpose of this study was to investigate the common bacteria isolate...

Full description

Saved in:
Bibliographic Details
Main Authors: Abba, Yusuf, Ilyasu, Yusuf Maina, Mohamed Noordin, Mustapha
Format: Article
Language:English
Published: Elsevier 2017
Online Access:http://psasir.upm.edu.my/id/eprint/62235/1/Isolation%20and%20identification%20of%20bacterial%20populations%20of%20zoonotic%20importance%20from%20captive%20non-venomous%20snakes%20in%20Malaysia.pdf
http://psasir.upm.edu.my/id/eprint/62235/
https://www.sciencedirect.com/science/article/pii/S0882401017303571?via%3Dihub
Tags: Add Tag
No Tags, Be the first to tag this record!
id my.upm.eprints.62235
record_format eprints
spelling my.upm.eprints.622352019-05-21T04:42:14Z http://psasir.upm.edu.my/id/eprint/62235/ Isolation and identification of bacterial populations of zoonotic importance from captive non-venomous snakes in Malaysia Abba, Yusuf Ilyasu, Yusuf Maina Mohamed Noordin, Mustapha Aim: Captivity of non-venomous snakes such as python and boa are common in zoos, aquariums and as pets in households. Poor captivity conditions expose these reptiles to numerous pathogens which may result in disease conditions. The purpose of this study was to investigate the common bacteria isolated from necropsied captive snakes in Malaysia over a five year period. Materials and methods: A total of 27 snake carcasses presented for necropsy at the Universiti Putra Malaysia (UPM) were used in this survey. Samples were aseptically obtained at necropsy from different organs/tissues (lung, liver, heart, kindey, oesophagus, lymph node, stomach, spinal cord, spleen, intestine) and cultured onto 5% blood and McConkey agar, respectively. Gram staining, morphological evaluation and biochemical test such as oxidase, catalase and coagulase were used to tentatively identify the presumptive bacterial isolates. Results: Pythons had the highest number of cases (81.3%) followed by anaconda (14.8%) and boa (3.7%). Mixed infection accounted for 81.5% in all snakes and was highest in pythons (63%). However, single infection was only observed in pythons (18.5%). A total of 82.7%, 95.4% and 100% of the bacterial isolates from python, anaconda and boa, respectively were gram negative. Aeromonas spp was the most frequently isolated bacteria in pythons and anaconda with incidences of 25 (18%) and 8 (36.6%) with no difference (p > 0.05) in incidence, respectively, while Salmonella spp was the most frequently isolated in boa and significantly higher (p < 0.05) than in python and anaconda. Bacteria species were most frequently isolated from the kidney of pythons 35 (25.2%), intestines of anacondas 11 (50%) and stomach of boa 3 (30%).Conclusion: This study showed that captive pythons harbored more bacterial speciesthan anaconda or boa. Most of the bacterial species isolated from these snakes have public health importance and have been incriminated in human infections worldwide. Elsevier 2017-07 Article PeerReviewed text en http://psasir.upm.edu.my/id/eprint/62235/1/Isolation%20and%20identification%20of%20bacterial%20populations%20of%20zoonotic%20importance%20from%20captive%20non-venomous%20snakes%20in%20Malaysia.pdf Abba, Yusuf and Ilyasu, Yusuf Maina and Mohamed Noordin, Mustapha (2017) Isolation and identification of bacterial populations of zoonotic importance from captive non-venomous snakes in Malaysia. Microbial Pathogenesis, 108. 49 - 54. ISSN 0882-4010; ESSN: 1096-1208 https://www.sciencedirect.com/science/article/pii/S0882401017303571?via%3Dihub 10.1016/j.micpath.2017.04.038
institution Universiti Putra Malaysia
building UPM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Putra Malaysia
content_source UPM Institutional Repository
url_provider http://psasir.upm.edu.my/
language English
description Aim: Captivity of non-venomous snakes such as python and boa are common in zoos, aquariums and as pets in households. Poor captivity conditions expose these reptiles to numerous pathogens which may result in disease conditions. The purpose of this study was to investigate the common bacteria isolated from necropsied captive snakes in Malaysia over a five year period. Materials and methods: A total of 27 snake carcasses presented for necropsy at the Universiti Putra Malaysia (UPM) were used in this survey. Samples were aseptically obtained at necropsy from different organs/tissues (lung, liver, heart, kindey, oesophagus, lymph node, stomach, spinal cord, spleen, intestine) and cultured onto 5% blood and McConkey agar, respectively. Gram staining, morphological evaluation and biochemical test such as oxidase, catalase and coagulase were used to tentatively identify the presumptive bacterial isolates. Results: Pythons had the highest number of cases (81.3%) followed by anaconda (14.8%) and boa (3.7%). Mixed infection accounted for 81.5% in all snakes and was highest in pythons (63%). However, single infection was only observed in pythons (18.5%). A total of 82.7%, 95.4% and 100% of the bacterial isolates from python, anaconda and boa, respectively were gram negative. Aeromonas spp was the most frequently isolated bacteria in pythons and anaconda with incidences of 25 (18%) and 8 (36.6%) with no difference (p > 0.05) in incidence, respectively, while Salmonella spp was the most frequently isolated in boa and significantly higher (p < 0.05) than in python and anaconda. Bacteria species were most frequently isolated from the kidney of pythons 35 (25.2%), intestines of anacondas 11 (50%) and stomach of boa 3 (30%).Conclusion: This study showed that captive pythons harbored more bacterial speciesthan anaconda or boa. Most of the bacterial species isolated from these snakes have public health importance and have been incriminated in human infections worldwide.
format Article
author Abba, Yusuf
Ilyasu, Yusuf Maina
Mohamed Noordin, Mustapha
spellingShingle Abba, Yusuf
Ilyasu, Yusuf Maina
Mohamed Noordin, Mustapha
Isolation and identification of bacterial populations of zoonotic importance from captive non-venomous snakes in Malaysia
author_facet Abba, Yusuf
Ilyasu, Yusuf Maina
Mohamed Noordin, Mustapha
author_sort Abba, Yusuf
title Isolation and identification of bacterial populations of zoonotic importance from captive non-venomous snakes in Malaysia
title_short Isolation and identification of bacterial populations of zoonotic importance from captive non-venomous snakes in Malaysia
title_full Isolation and identification of bacterial populations of zoonotic importance from captive non-venomous snakes in Malaysia
title_fullStr Isolation and identification of bacterial populations of zoonotic importance from captive non-venomous snakes in Malaysia
title_full_unstemmed Isolation and identification of bacterial populations of zoonotic importance from captive non-venomous snakes in Malaysia
title_sort isolation and identification of bacterial populations of zoonotic importance from captive non-venomous snakes in malaysia
publisher Elsevier
publishDate 2017
url http://psasir.upm.edu.my/id/eprint/62235/1/Isolation%20and%20identification%20of%20bacterial%20populations%20of%20zoonotic%20importance%20from%20captive%20non-venomous%20snakes%20in%20Malaysia.pdf
http://psasir.upm.edu.my/id/eprint/62235/
https://www.sciencedirect.com/science/article/pii/S0882401017303571?via%3Dihub
_version_ 1643837702266683392
score 13.211869

Similar Items