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Inhibition of cell migration and invasion by miR-29a-3p in a colorectal cancer cell line through suppression of CDC42BPA mRNA expression

The objective of this study was to determine the effect of miR 29a 3p inhibitor on the migration and invasion of colorectal cancer cell lines (CRC) and the underlying molecular mechanisms. miR 29a 3p was detected using reverse transcription-quantitative polymerase chain reaction (RT qPCR) in the CRC...

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Main Authors: He, Pei Yuan, Yip, Wai Kien, Chai, Boon Lee, Chai, Boon Yean, Jabar, Mohd Faisal, Dusa, Noraini, Mohtarrudin, Norhafizah, Seow, Heng Fong
Format: Article
Language:English
Published: Spandidos Publications 2017
Online Access:http://psasir.upm.edu.my/id/eprint/62177/1/Inhibition%20of%20cell%20migration%20and%20invasion%20by%20miR%2029a%203p%20in%20a%20colorectal%20cancer%20cell%20line%20through%20suppression%20of%20CDC42BPA%20mRNA%20expression.pdf
http://psasir.upm.edu.my/id/eprint/62177/
https://www.spandidos-publications.com/or/38/6/3554
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spelling my.upm.eprints.621772019-04-18T07:53:15Z http://psasir.upm.edu.my/id/eprint/62177/ Inhibition of cell migration and invasion by miR-29a-3p in a colorectal cancer cell line through suppression of CDC42BPA mRNA expression He, Pei Yuan Yip, Wai Kien Chai, Boon Lee Chai, Boon Yean Jabar, Mohd Faisal Dusa, Noraini Mohtarrudin, Norhafizah Seow, Heng Fong The objective of this study was to determine the effect of miR 29a 3p inhibitor on the migration and invasion of colorectal cancer cell lines (CRC) and the underlying molecular mechanisms. miR 29a 3p was detected using reverse transcription-quantitative polymerase chain reaction (RT qPCR) in the CRC cell lines HCT11, CaCo2, HT29, SW480 and SW620. An invasive subpopulation designated SW480 7 was derived from the parental cell line, detected by Transwell and Transwell Matrigel assays. Cytoskeleton Regulators RT2 profiler PCR array and western blot analysis were utilized to identify the alterations in expression of downstream mRNAs. siRNA against CDC42BPA was transfected into SW480 7 and effects on cell migration and invasion were investigated. Data obtained showed that miR 29a 3p was detected in these five CRC cell lines. miR 29a 3p inhibitor had no effect on viability but stimulated cell migration and invasion of SW480 7 cells. In contrast, miR 29a 3p mimic suppressed cell migration and invasion. TargetScan miRBD and DIANA were employed to identify the potential direct target genes of miR 29a 3p in the Cytoskeleton Regulators RT2-Profiler PCR array. Cytoskeleton Regulators RT2-Profiler PCR array data showed that 3 out of the 5 predicted targets genes, CDC42BPA (2.33-fold), BAIAP2 (1.79-fold) and TIAM1 (1.77-fold), in the array were upregulated by miR 29a 3p. A significant increase in expression IQGAP2, PHLDB2, SSH1 mRNAs and downregulation of PAK1 mRNA was also detected with miR 29a 3p inhibition. Increase in CDC42BPA, SSH1 and IQGAP2 mRNA expression correlated with increased protein level in miR 29a 3p transfected SW-480-7 cells. Silencing of CDC42BPA (an enhancer of cell motility) partially abolished miR 29a 3p inhibitor-induced stimulation of cell migration and invasion. miR 29a 3p expression in stage II and III CRC is relatively lower than that of stage I CRC. However, the data need to be interpreted with caution due to the small sample size. In conclusion, inhibition of miR 29a 3p stimulates SW480 7 cell migration and invasion and downstream expression IQGAP2, PHLDB2, SSH1 mRNAs are upregulated whilst PAK1 mRNA is downregulated. Silencing of CDC42BPA expression partially reduces miR29a 3p inhibitor-induced migration and invasion of SW480 7 cells. Spandidos Publications 2017 Article PeerReviewed text en http://psasir.upm.edu.my/id/eprint/62177/1/Inhibition%20of%20cell%20migration%20and%20invasion%20by%20miR%2029a%203p%20in%20a%20colorectal%20cancer%20cell%20line%20through%20suppression%20of%20CDC42BPA%20mRNA%20expression.pdf He, Pei Yuan and Yip, Wai Kien and Chai, Boon Lee and Chai, Boon Yean and Jabar, Mohd Faisal and Dusa, Noraini and Mohtarrudin, Norhafizah and Seow, Heng Fong (2017) Inhibition of cell migration and invasion by miR-29a-3p in a colorectal cancer cell line through suppression of CDC42BPA mRNA expression. Oncology reports, 38 (6). 3554 - 3566. ISSN 1021-335X, ESSN: 1791-2431 https://www.spandidos-publications.com/or/38/6/3554 10.3892/or.2017.6037
institution Universiti Putra Malaysia
building UPM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Putra Malaysia
content_source UPM Institutional Repository
url_provider http://psasir.upm.edu.my/
language English
description The objective of this study was to determine the effect of miR 29a 3p inhibitor on the migration and invasion of colorectal cancer cell lines (CRC) and the underlying molecular mechanisms. miR 29a 3p was detected using reverse transcription-quantitative polymerase chain reaction (RT qPCR) in the CRC cell lines HCT11, CaCo2, HT29, SW480 and SW620. An invasive subpopulation designated SW480 7 was derived from the parental cell line, detected by Transwell and Transwell Matrigel assays. Cytoskeleton Regulators RT2 profiler PCR array and western blot analysis were utilized to identify the alterations in expression of downstream mRNAs. siRNA against CDC42BPA was transfected into SW480 7 and effects on cell migration and invasion were investigated. Data obtained showed that miR 29a 3p was detected in these five CRC cell lines. miR 29a 3p inhibitor had no effect on viability but stimulated cell migration and invasion of SW480 7 cells. In contrast, miR 29a 3p mimic suppressed cell migration and invasion. TargetScan miRBD and DIANA were employed to identify the potential direct target genes of miR 29a 3p in the Cytoskeleton Regulators RT2-Profiler PCR array. Cytoskeleton Regulators RT2-Profiler PCR array data showed that 3 out of the 5 predicted targets genes, CDC42BPA (2.33-fold), BAIAP2 (1.79-fold) and TIAM1 (1.77-fold), in the array were upregulated by miR 29a 3p. A significant increase in expression IQGAP2, PHLDB2, SSH1 mRNAs and downregulation of PAK1 mRNA was also detected with miR 29a 3p inhibition. Increase in CDC42BPA, SSH1 and IQGAP2 mRNA expression correlated with increased protein level in miR 29a 3p transfected SW-480-7 cells. Silencing of CDC42BPA (an enhancer of cell motility) partially abolished miR 29a 3p inhibitor-induced stimulation of cell migration and invasion. miR 29a 3p expression in stage II and III CRC is relatively lower than that of stage I CRC. However, the data need to be interpreted with caution due to the small sample size. In conclusion, inhibition of miR 29a 3p stimulates SW480 7 cell migration and invasion and downstream expression IQGAP2, PHLDB2, SSH1 mRNAs are upregulated whilst PAK1 mRNA is downregulated. Silencing of CDC42BPA expression partially reduces miR29a 3p inhibitor-induced migration and invasion of SW480 7 cells.
format Article
author He, Pei Yuan
Yip, Wai Kien
Chai, Boon Lee
Chai, Boon Yean
Jabar, Mohd Faisal
Dusa, Noraini
Mohtarrudin, Norhafizah
Seow, Heng Fong
spellingShingle He, Pei Yuan
Yip, Wai Kien
Chai, Boon Lee
Chai, Boon Yean
Jabar, Mohd Faisal
Dusa, Noraini
Mohtarrudin, Norhafizah
Seow, Heng Fong
Inhibition of cell migration and invasion by miR-29a-3p in a colorectal cancer cell line through suppression of CDC42BPA mRNA expression
author_facet He, Pei Yuan
Yip, Wai Kien
Chai, Boon Lee
Chai, Boon Yean
Jabar, Mohd Faisal
Dusa, Noraini
Mohtarrudin, Norhafizah
Seow, Heng Fong
author_sort He, Pei Yuan
title Inhibition of cell migration and invasion by miR-29a-3p in a colorectal cancer cell line through suppression of CDC42BPA mRNA expression
title_short Inhibition of cell migration and invasion by miR-29a-3p in a colorectal cancer cell line through suppression of CDC42BPA mRNA expression
title_full Inhibition of cell migration and invasion by miR-29a-3p in a colorectal cancer cell line through suppression of CDC42BPA mRNA expression
title_fullStr Inhibition of cell migration and invasion by miR-29a-3p in a colorectal cancer cell line through suppression of CDC42BPA mRNA expression
title_full_unstemmed Inhibition of cell migration and invasion by miR-29a-3p in a colorectal cancer cell line through suppression of CDC42BPA mRNA expression
title_sort inhibition of cell migration and invasion by mir-29a-3p in a colorectal cancer cell line through suppression of cdc42bpa mrna expression
publisher Spandidos Publications
publishDate 2017
url http://psasir.upm.edu.my/id/eprint/62177/1/Inhibition%20of%20cell%20migration%20and%20invasion%20by%20miR%2029a%203p%20in%20a%20colorectal%20cancer%20cell%20line%20through%20suppression%20of%20CDC42BPA%20mRNA%20expression.pdf
http://psasir.upm.edu.my/id/eprint/62177/
https://www.spandidos-publications.com/or/38/6/3554
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score 13.211869

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