Impact of signal peptide and transmembrane segments on expression and biochemical properties of a lipase from Bacillus sphaericus 205y
A total of 97 amino acids, considered as the signal peptide and transmembrane segments were removed from 205y lipase gene using polymerase chain reaction technique that abolished the low activity of this enzyme. The mature enzyme was expressed in Escherichia coliusing pBAD expression vector, which g...
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| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Elsevier
2017
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| Online Access: | http://psasir.upm.edu.my/id/eprint/62103/1/Impact%20of%20signal%20peptide%20and%20transmembrane%20segments%20on%20expression%20and%20biochemical%20properties%20of%20a%20lipase%20from%20Bacillus%20sphaericus%20205y.pdf http://psasir.upm.edu.my/id/eprint/62103/ https://www.sciencedirect.com/science/article/pii/S0168165617317091?via%3Dihub |
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http://psasir.upm.edu.my/id/eprint/62103/1/Impact%20of%20signal%20peptide%20and%20transmembrane%20segments%20on%20expression%20and%20biochemical%20properties%20of%20a%20lipase%20from%20Bacillus%20sphaericus%20205y.pdfhttp://psasir.upm.edu.my/id/eprint/62103/
https://www.sciencedirect.com/science/article/pii/S0168165617317091?via%3Dihub