Cover Image

Identification of Trichoderma harzianum T3.13 and its interaction with Neoscytalidium dimidiatum U1, a pathogenic fungus isolated from dragon fruit (Hylocereus polyrhizus) in Malaysia

Endophytes can produce enzymes which facilitate their initial colonisation of plant tissues and direct interactions with microbial pathogens. In this study, endophytic fungus from the stem of healthy dragon fruit (Hylocereus spp.) was successfully identified as Trichoderma harzianum T3.13. T. harzia...

Full description

Saved in:
Bibliographic Details
Main Authors: Wan Zulkifeli, Wan Rusmarini, Md Shah, Umi Kalsom, Abdullah, Mohd Puad, Mamat, Suhaila, Tan, Geok Hun
Format: Article
Language:English
Published: International Journal of Agriculture and Environmental Research 2017
Online Access:http://psasir.upm.edu.my/id/eprint/62074/1/Identification%20of%20Trichoderma%20harzianum%20T3.13%20and%20its%20interaction%20with%20Neoscytalidium%20dimidiatum%20U1.pdf
http://psasir.upm.edu.my/id/eprint/62074/
https://ijaer.in/more2.php?id=225
Tags: Add Tag
No Tags, Be the first to tag this record!
id my.upm.eprints.62074
record_format eprints
spelling my.upm.eprints.620742019-03-22T07:51:03Z http://psasir.upm.edu.my/id/eprint/62074/ Identification of Trichoderma harzianum T3.13 and its interaction with Neoscytalidium dimidiatum U1, a pathogenic fungus isolated from dragon fruit (Hylocereus polyrhizus) in Malaysia Wan Zulkifeli, Wan Rusmarini Md Shah, Umi Kalsom Abdullah, Mohd Puad Mamat, Suhaila Tan, Geok Hun Endophytes can produce enzymes which facilitate their initial colonisation of plant tissues and direct interactions with microbial pathogens. In this study, endophytic fungus from the stem of healthy dragon fruit (Hylocereus spp.) was successfully identified as Trichoderma harzianum T3.13. T. harzianum T3.13 was shown to have the ability to produce antagonistic activity against Neoscytalidium dimidiatum U1, a pathogen fungus from the stem of unhealthy dragon fruit. The chitinolytic activities of T. harzianum T3.13 were 0.194 U/ml in a medium containing 3% (w/v) of colloidal chitin as sole carbon source. Semi-quantitative RT-PCR was used to quantify the expression patterns of the genes during the interaction of T. harzianum T3.13 with pathogen N. dimidiatum U1 and control pathogen Colletotrichum gloeosporioides, respectively. The expression of the exc1 and chit42 genes were observed to be present before and after the interaction occurred in the presence of N. dimidiatum U1. However, the expression of the bgn13.1 gene increased after 24 hours up to 96 hours of interaction in the presence of N. dimidiatum U1. In the presence of C. gloeosporioides, the expression of bgn13.1 and chit42 gradually decreased during the interaction although the expression of the exc1 gene did not change. The results suggested that the endophytic fungus T. harzianum T3.13 has the potential as a good biological control agent against N. dimidiatum U1 and C. gloeosporioides. Thus, the study provided an insight into cellular and molecular interactions between T. harzianum T3.13 and pathogenic fungi. International Journal of Agriculture and Environmental Research 2017-06 Article PeerReviewed text en http://psasir.upm.edu.my/id/eprint/62074/1/Identification%20of%20Trichoderma%20harzianum%20T3.13%20and%20its%20interaction%20with%20Neoscytalidium%20dimidiatum%20U1.pdf Wan Zulkifeli, Wan Rusmarini and Md Shah, Umi Kalsom and Abdullah, Mohd Puad and Mamat, Suhaila and Tan, Geok Hun (2017) Identification of Trichoderma harzianum T3.13 and its interaction with Neoscytalidium dimidiatum U1, a pathogenic fungus isolated from dragon fruit (Hylocereus polyrhizus) in Malaysia. International Journal of Agriculture and Environmental Research, 3 (3). pp. 3205-3228. ISSN 2455-6939 https://ijaer.in/more2.php?id=225
institution Universiti Putra Malaysia
building UPM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Putra Malaysia
content_source UPM Institutional Repository
url_provider http://psasir.upm.edu.my/
language English
description Endophytes can produce enzymes which facilitate their initial colonisation of plant tissues and direct interactions with microbial pathogens. In this study, endophytic fungus from the stem of healthy dragon fruit (Hylocereus spp.) was successfully identified as Trichoderma harzianum T3.13. T. harzianum T3.13 was shown to have the ability to produce antagonistic activity against Neoscytalidium dimidiatum U1, a pathogen fungus from the stem of unhealthy dragon fruit. The chitinolytic activities of T. harzianum T3.13 were 0.194 U/ml in a medium containing 3% (w/v) of colloidal chitin as sole carbon source. Semi-quantitative RT-PCR was used to quantify the expression patterns of the genes during the interaction of T. harzianum T3.13 with pathogen N. dimidiatum U1 and control pathogen Colletotrichum gloeosporioides, respectively. The expression of the exc1 and chit42 genes were observed to be present before and after the interaction occurred in the presence of N. dimidiatum U1. However, the expression of the bgn13.1 gene increased after 24 hours up to 96 hours of interaction in the presence of N. dimidiatum U1. In the presence of C. gloeosporioides, the expression of bgn13.1 and chit42 gradually decreased during the interaction although the expression of the exc1 gene did not change. The results suggested that the endophytic fungus T. harzianum T3.13 has the potential as a good biological control agent against N. dimidiatum U1 and C. gloeosporioides. Thus, the study provided an insight into cellular and molecular interactions between T. harzianum T3.13 and pathogenic fungi.
format Article
author Wan Zulkifeli, Wan Rusmarini
Md Shah, Umi Kalsom
Abdullah, Mohd Puad
Mamat, Suhaila
Tan, Geok Hun
spellingShingle Wan Zulkifeli, Wan Rusmarini
Md Shah, Umi Kalsom
Abdullah, Mohd Puad
Mamat, Suhaila
Tan, Geok Hun
Identification of Trichoderma harzianum T3.13 and its interaction with Neoscytalidium dimidiatum U1, a pathogenic fungus isolated from dragon fruit (Hylocereus polyrhizus) in Malaysia
author_facet Wan Zulkifeli, Wan Rusmarini
Md Shah, Umi Kalsom
Abdullah, Mohd Puad
Mamat, Suhaila
Tan, Geok Hun
author_sort Wan Zulkifeli, Wan Rusmarini
title Identification of Trichoderma harzianum T3.13 and its interaction with Neoscytalidium dimidiatum U1, a pathogenic fungus isolated from dragon fruit (Hylocereus polyrhizus) in Malaysia
title_short Identification of Trichoderma harzianum T3.13 and its interaction with Neoscytalidium dimidiatum U1, a pathogenic fungus isolated from dragon fruit (Hylocereus polyrhizus) in Malaysia
title_full Identification of Trichoderma harzianum T3.13 and its interaction with Neoscytalidium dimidiatum U1, a pathogenic fungus isolated from dragon fruit (Hylocereus polyrhizus) in Malaysia
title_fullStr Identification of Trichoderma harzianum T3.13 and its interaction with Neoscytalidium dimidiatum U1, a pathogenic fungus isolated from dragon fruit (Hylocereus polyrhizus) in Malaysia
title_full_unstemmed Identification of Trichoderma harzianum T3.13 and its interaction with Neoscytalidium dimidiatum U1, a pathogenic fungus isolated from dragon fruit (Hylocereus polyrhizus) in Malaysia
title_sort identification of trichoderma harzianum t3.13 and its interaction with neoscytalidium dimidiatum u1, a pathogenic fungus isolated from dragon fruit (hylocereus polyrhizus) in malaysia
publisher International Journal of Agriculture and Environmental Research
publishDate 2017
url http://psasir.upm.edu.my/id/eprint/62074/1/Identification%20of%20Trichoderma%20harzianum%20T3.13%20and%20its%20interaction%20with%20Neoscytalidium%20dimidiatum%20U1.pdf
http://psasir.upm.edu.my/id/eprint/62074/
https://ijaer.in/more2.php?id=225
_version_ 1643837662974443520
score 13.211869

Similar Items