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Floral micromorphology and transcriptome analyses of a fragrant Vandaceous Orchid, Vanda Mimi Palmer, for its fragrance production sites

Background: Vanda Mimi Palmer (VMP) is commercially valuable for its strong fragrance but little is known regarding the fragrance production and emission sites on the fowers. Results: Olfactory perception detected fragrance only from the petals and sepals. Light and Environmental Scanning Electron m...

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Main Authors: Toh, Conie, Ab. Rahim, Mohd Hairul, Mohd. Ain, Nooraini, Namasivayam, Parameswari, Go, Rusea, Abdullah, Nur Ashikin Psyquay, Abdullah, Meilina Ong, Abdullah, Janna Ong
Format: Article
Language:English
Published: BioMed Central 2017
Online Access:http://psasir.upm.edu.my/id/eprint/61899/1/Floral%20micromorphology%20and%20transcriptome%20analyses.pdf
http://psasir.upm.edu.my/id/eprint/61899/
https://bmcresnotes.biomedcentral.com/articles/10.1186/s13104-017-2872-6
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spelling my.upm.eprints.618992019-02-26T08:28:23Z http://psasir.upm.edu.my/id/eprint/61899/ Floral micromorphology and transcriptome analyses of a fragrant Vandaceous Orchid, Vanda Mimi Palmer, for its fragrance production sites Toh, Conie Ab. Rahim, Mohd Hairul Mohd. Ain, Nooraini Namasivayam, Parameswari Go, Rusea Abdullah, Nur Ashikin Psyquay Abdullah, Meilina Ong Abdullah, Janna Ong Background: Vanda Mimi Palmer (VMP) is commercially valuable for its strong fragrance but little is known regarding the fragrance production and emission sites on the fowers. Results: Olfactory perception detected fragrance only from the petals and sepals. Light and Environmental Scanning Electron microscopy analyses on fresh tissues showed distributions of stomata and trichomes concentrated mostly around the edges. These results paralleled the rich starch deposits and intense neutral red stain, indicating strong fragrance and trichomes as potential main fragrance release sites. Next Generation Sequencing (NGS) transcriptomic data of adaxial and abaxial layers of the tissues showed monoterpene synthase transcripts specifcally linalool and ocimene synthases distributed throughout the tissues. qPCR analyses taken at diferent time points revealed high levels of linalool and ocimene synthases transcripts in the early morning with maximal level at 4.00 am but remained low throughout daylight hours. Conclusions: Knowledge of the VMP foral anatomy and its fragrance production characteristics, which complemented our previous molecular and biochemical data on VMP, provided additional knowledge on how fragrance and fower morphology are closely intertwined. Further investigation on the mechanisms of fragrance biosynthesis and interaction of potential pollinators would elucidate the evolution of the fower morphology to maximize the reproduction success of this plant. BioMed Central 2017 Article PeerReviewed text en http://psasir.upm.edu.my/id/eprint/61899/1/Floral%20micromorphology%20and%20transcriptome%20analyses.pdf Toh, Conie and Ab. Rahim, Mohd Hairul and Mohd. Ain, Nooraini and Namasivayam, Parameswari and Go, Rusea and Abdullah, Nur Ashikin Psyquay and Abdullah, Meilina Ong and Abdullah, Janna Ong (2017) Floral micromorphology and transcriptome analyses of a fragrant Vandaceous Orchid, Vanda Mimi Palmer, for its fragrance production sites. BMC Research Notes, 10. pp. 1-10. ISSN 1756-0500 https://bmcresnotes.biomedcentral.com/articles/10.1186/s13104-017-2872-6 10.1186/s13104-017-2872-6
institution Universiti Putra Malaysia
building UPM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Putra Malaysia
content_source UPM Institutional Repository
url_provider http://psasir.upm.edu.my/
language English
description Background: Vanda Mimi Palmer (VMP) is commercially valuable for its strong fragrance but little is known regarding the fragrance production and emission sites on the fowers. Results: Olfactory perception detected fragrance only from the petals and sepals. Light and Environmental Scanning Electron microscopy analyses on fresh tissues showed distributions of stomata and trichomes concentrated mostly around the edges. These results paralleled the rich starch deposits and intense neutral red stain, indicating strong fragrance and trichomes as potential main fragrance release sites. Next Generation Sequencing (NGS) transcriptomic data of adaxial and abaxial layers of the tissues showed monoterpene synthase transcripts specifcally linalool and ocimene synthases distributed throughout the tissues. qPCR analyses taken at diferent time points revealed high levels of linalool and ocimene synthases transcripts in the early morning with maximal level at 4.00 am but remained low throughout daylight hours. Conclusions: Knowledge of the VMP foral anatomy and its fragrance production characteristics, which complemented our previous molecular and biochemical data on VMP, provided additional knowledge on how fragrance and fower morphology are closely intertwined. Further investigation on the mechanisms of fragrance biosynthesis and interaction of potential pollinators would elucidate the evolution of the fower morphology to maximize the reproduction success of this plant.
format Article
author Toh, Conie
Ab. Rahim, Mohd Hairul
Mohd. Ain, Nooraini
Namasivayam, Parameswari
Go, Rusea
Abdullah, Nur Ashikin Psyquay
Abdullah, Meilina Ong
Abdullah, Janna Ong
spellingShingle Toh, Conie
Ab. Rahim, Mohd Hairul
Mohd. Ain, Nooraini
Namasivayam, Parameswari
Go, Rusea
Abdullah, Nur Ashikin Psyquay
Abdullah, Meilina Ong
Abdullah, Janna Ong
Floral micromorphology and transcriptome analyses of a fragrant Vandaceous Orchid, Vanda Mimi Palmer, for its fragrance production sites
author_facet Toh, Conie
Ab. Rahim, Mohd Hairul
Mohd. Ain, Nooraini
Namasivayam, Parameswari
Go, Rusea
Abdullah, Nur Ashikin Psyquay
Abdullah, Meilina Ong
Abdullah, Janna Ong
author_sort Toh, Conie
title Floral micromorphology and transcriptome analyses of a fragrant Vandaceous Orchid, Vanda Mimi Palmer, for its fragrance production sites
title_short Floral micromorphology and transcriptome analyses of a fragrant Vandaceous Orchid, Vanda Mimi Palmer, for its fragrance production sites
title_full Floral micromorphology and transcriptome analyses of a fragrant Vandaceous Orchid, Vanda Mimi Palmer, for its fragrance production sites
title_fullStr Floral micromorphology and transcriptome analyses of a fragrant Vandaceous Orchid, Vanda Mimi Palmer, for its fragrance production sites
title_full_unstemmed Floral micromorphology and transcriptome analyses of a fragrant Vandaceous Orchid, Vanda Mimi Palmer, for its fragrance production sites
title_sort floral micromorphology and transcriptome analyses of a fragrant vandaceous orchid, vanda mimi palmer, for its fragrance production sites
publisher BioMed Central
publishDate 2017
url http://psasir.upm.edu.my/id/eprint/61899/1/Floral%20micromorphology%20and%20transcriptome%20analyses.pdf
http://psasir.upm.edu.my/id/eprint/61899/
https://bmcresnotes.biomedcentral.com/articles/10.1186/s13104-017-2872-6
_version_ 1643837620588904448
score 13.211869

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