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Factors affecting shoot and root apical meristem tissue culture of Thai supersweet corn (Zea mays var. rugosa)

During last recent years, in vitro propagation technic is widely used to produce plants with desirable traits. This experiment was conducted to produce an ideal protocol for in vitro propagation of Thai supersweet corn by using shoot apical meristem (SAM) and root apical meristem (RAM) as explants....

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Main Authors: Silvarajana, Lavanya, Kalhori, Nahid, Nulit, Rosimah, Qamaruz Zaman, Faridah, Rambod, Abiri, Atabaki, Narges
Format: Article
Language:English
Published: Taylor & Francis Group 2017
Online Access:http://psasir.upm.edu.my/id/eprint/61846/1/Factors%20affecting%20shoot%20and%20root%20apical%20meristem%20tissue%20culture%20of%20Thai%20supersweet%20corn.pdf
http://psasir.upm.edu.my/id/eprint/61846/
https://www.tandfonline.com/doi/abs/10.1080/01904167.2017.1310889
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spelling my.upm.eprints.618462019-02-20T09:10:00Z http://psasir.upm.edu.my/id/eprint/61846/ Factors affecting shoot and root apical meristem tissue culture of Thai supersweet corn (Zea mays var. rugosa) Silvarajana, Lavanya Kalhori, Nahid Nulit, Rosimah Qamaruz Zaman, Faridah Rambod, Abiri Atabaki, Narges During last recent years, in vitro propagation technic is widely used to produce plants with desirable traits. This experiment was conducted to produce an ideal protocol for in vitro propagation of Thai supersweet corn by using shoot apical meristem (SAM) and root apical meristem (RAM) as explants. Four-day-old germinating seedlings were used as the experimental materials on culture media supplemented with a range of auxin, kinetin, and carbohydrates. The primary establishment for SAM showed the highest percentage of survival (80%) while RAM showed the highest survival (67%) and in Murashige and Skoog (MS) media supplemented. Upon acclimatization, regenerated plantlets from shoot showed the highest survival rate (12%) with the production of 21 plantlets; however, the survival rate of plantlets from root was only 20% with the production of 9 plantlets. The efficient and economic protocol that is produced in this study can be applied as an alternative to conventional propagation method for the large-scale production of Thai supersweet corn throughout the year. Taylor & Francis Group 2017 Article PeerReviewed text en http://psasir.upm.edu.my/id/eprint/61846/1/Factors%20affecting%20shoot%20and%20root%20apical%20meristem%20tissue%20culture%20of%20Thai%20supersweet%20corn.pdf Silvarajana, Lavanya and Kalhori, Nahid and Nulit, Rosimah and Qamaruz Zaman, Faridah and Rambod, Abiri and Atabaki, Narges (2017) Factors affecting shoot and root apical meristem tissue culture of Thai supersweet corn (Zea mays var. rugosa). Journal of Plant Nutrition, 40 (12). 1677 - 1689. ISSN 0190-4167; ESSN: 1532-4087 https://www.tandfonline.com/doi/abs/10.1080/01904167.2017.1310889 10.1080/01904167.2017.1310889
institution Universiti Putra Malaysia
building UPM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Putra Malaysia
content_source UPM Institutional Repository
url_provider http://psasir.upm.edu.my/
language English
description During last recent years, in vitro propagation technic is widely used to produce plants with desirable traits. This experiment was conducted to produce an ideal protocol for in vitro propagation of Thai supersweet corn by using shoot apical meristem (SAM) and root apical meristem (RAM) as explants. Four-day-old germinating seedlings were used as the experimental materials on culture media supplemented with a range of auxin, kinetin, and carbohydrates. The primary establishment for SAM showed the highest percentage of survival (80%) while RAM showed the highest survival (67%) and in Murashige and Skoog (MS) media supplemented. Upon acclimatization, regenerated plantlets from shoot showed the highest survival rate (12%) with the production of 21 plantlets; however, the survival rate of plantlets from root was only 20% with the production of 9 plantlets. The efficient and economic protocol that is produced in this study can be applied as an alternative to conventional propagation method for the large-scale production of Thai supersweet corn throughout the year.
format Article
author Silvarajana, Lavanya
Kalhori, Nahid
Nulit, Rosimah
Qamaruz Zaman, Faridah
Rambod, Abiri
Atabaki, Narges
spellingShingle Silvarajana, Lavanya
Kalhori, Nahid
Nulit, Rosimah
Qamaruz Zaman, Faridah
Rambod, Abiri
Atabaki, Narges
Factors affecting shoot and root apical meristem tissue culture of Thai supersweet corn (Zea mays var. rugosa)
author_facet Silvarajana, Lavanya
Kalhori, Nahid
Nulit, Rosimah
Qamaruz Zaman, Faridah
Rambod, Abiri
Atabaki, Narges
author_sort Silvarajana, Lavanya
title Factors affecting shoot and root apical meristem tissue culture of Thai supersweet corn (Zea mays var. rugosa)
title_short Factors affecting shoot and root apical meristem tissue culture of Thai supersweet corn (Zea mays var. rugosa)
title_full Factors affecting shoot and root apical meristem tissue culture of Thai supersweet corn (Zea mays var. rugosa)
title_fullStr Factors affecting shoot and root apical meristem tissue culture of Thai supersweet corn (Zea mays var. rugosa)
title_full_unstemmed Factors affecting shoot and root apical meristem tissue culture of Thai supersweet corn (Zea mays var. rugosa)
title_sort factors affecting shoot and root apical meristem tissue culture of thai supersweet corn (zea mays var. rugosa)
publisher Taylor & Francis Group
publishDate 2017
url http://psasir.upm.edu.my/id/eprint/61846/1/Factors%20affecting%20shoot%20and%20root%20apical%20meristem%20tissue%20culture%20of%20Thai%20supersweet%20corn.pdf
http://psasir.upm.edu.my/id/eprint/61846/
https://www.tandfonline.com/doi/abs/10.1080/01904167.2017.1310889
_version_ 1643837610211147776
score 13.211869

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