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Endophytic Microorganisms for Improvement of Banana Vigour and Tolerance to Fusarium Wilt

This study explored the potential of endophytic microorganisms (EMS) isolated from wild bananas as biocontrol agents (BCAs) against Fusarium wilt caused by Fusarium oxysporum f. sp. cubense race 4 (FocR4) in susceptible banana ramets (Berangan cv. Intan). The fungal (Fusarium oxysporum (UPM31 PI...

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Main Author: Ting, Adeline Su Yien
Format: Thesis
Language:English
English
Published: 2005
Subjects:
Bananas - Case studies
Fusarium wilt of banana - Case studies
Online Access:http://psasir.upm.edu.my/id/eprint/6170/1/FP_2005_9.pdf
http://psasir.upm.edu.my/id/eprint/6170/
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spelling my.upm.eprints.61702023-10-12T03:22:17Z http://psasir.upm.edu.my/id/eprint/6170/ Endophytic Microorganisms for Improvement of Banana Vigour and Tolerance to Fusarium Wilt Ting, Adeline Su Yien This study explored the potential of endophytic microorganisms (EMS) isolated from wild bananas as biocontrol agents (BCAs) against Fusarium wilt caused by Fusarium oxysporum f. sp. cubense race 4 (FocR4) in susceptible banana ramets (Berangan cv. Intan). The fungal (Fusarium oxysporum (UPM31 PI)) and bacterial endophytes (Serratia marcescens (UPM39B3)) were established as effective BCAs; improving growth and vigour and enhancing tolerance of susceptible ramets to Fusarium wilt. They were antagonistic towards FocR4, with Percentage Inhibition of Radial Growth (PIRG) values of 65% and 63%, respectively. Inhibitory substances were produced in the form of volatile as well as non-volatile substances. The endophytes were also able to colonize the host tissues, including the roots and corms in artificial inoculation under laboratory conditions. The association of F. oxysporum (UPM31 PI) and S. marcescens (UPM39B3) with the host plants resulted in enhanced vegetative growth as shown by the increase in height, pseudostem diameter, root mass and total number of leaves ramet-'. Endophytes acted as elicitors in the production of inducible compounds associated with induced resistance (peroxidase, polyphenoloxidase, phenylalanine ammonia lyase, total soluble phenol and lignin content). The robust growth and occurrence of induced resistance subsequently enhanced tolerance of the ramets to Fusarium wilt based on parameters such as delay in onset of symptoms, lower percentages in disease incidence, disease severity, and epidemic rate. Inoculation with F. oxysporum singly was most effective followed by inoculation with mixture with S. marcescens (UPM31 Pl+UPM39B3). However, the survival and subsequent biocontrol efficacy of F. oxysporum (UPM31 PI) and S. marcescens (UPM39B3) might be vulnerable to the changing soil and environmental conditions. Populations of endophytes were not sustained over time. Therefore, further studies regarding formulation and application frequency and techniques, are essential to maximize the potential of F. oxysporum (UPM31P1) and S. marcescens (UPM39B3) as BCAs against Fusarium wilt of banana. 2005-10 Thesis NonPeerReviewed text en http://psasir.upm.edu.my/id/eprint/6170/1/FP_2005_9.pdf Ting, Adeline Su Yien (2005) Endophytic Microorganisms for Improvement of Banana Vigour and Tolerance to Fusarium Wilt. Doctoral thesis, Universiti Putra Malaysia. Bananas - Case studies Fusarium wilt of banana - Case studies English
institution Universiti Putra Malaysia
building UPM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Putra Malaysia
content_source UPM Institutional Repository
url_provider http://psasir.upm.edu.my/
language English
English
topic Bananas - Case studies
Fusarium wilt of banana - Case studies
spellingShingle Bananas - Case studies
Fusarium wilt of banana - Case studies
Ting, Adeline Su Yien
Endophytic Microorganisms for Improvement of Banana Vigour and Tolerance to Fusarium Wilt
description This study explored the potential of endophytic microorganisms (EMS) isolated from wild bananas as biocontrol agents (BCAs) against Fusarium wilt caused by Fusarium oxysporum f. sp. cubense race 4 (FocR4) in susceptible banana ramets (Berangan cv. Intan). The fungal (Fusarium oxysporum (UPM31 PI)) and bacterial endophytes (Serratia marcescens (UPM39B3)) were established as effective BCAs; improving growth and vigour and enhancing tolerance of susceptible ramets to Fusarium wilt. They were antagonistic towards FocR4, with Percentage Inhibition of Radial Growth (PIRG) values of 65% and 63%, respectively. Inhibitory substances were produced in the form of volatile as well as non-volatile substances. The endophytes were also able to colonize the host tissues, including the roots and corms in artificial inoculation under laboratory conditions. The association of F. oxysporum (UPM31 PI) and S. marcescens (UPM39B3) with the host plants resulted in enhanced vegetative growth as shown by the increase in height, pseudostem diameter, root mass and total number of leaves ramet-'. Endophytes acted as elicitors in the production of inducible compounds associated with induced resistance (peroxidase, polyphenoloxidase, phenylalanine ammonia lyase, total soluble phenol and lignin content). The robust growth and occurrence of induced resistance subsequently enhanced tolerance of the ramets to Fusarium wilt based on parameters such as delay in onset of symptoms, lower percentages in disease incidence, disease severity, and epidemic rate. Inoculation with F. oxysporum singly was most effective followed by inoculation with mixture with S. marcescens (UPM31 Pl+UPM39B3). However, the survival and subsequent biocontrol efficacy of F. oxysporum (UPM31 PI) and S. marcescens (UPM39B3) might be vulnerable to the changing soil and environmental conditions. Populations of endophytes were not sustained over time. Therefore, further studies regarding formulation and application frequency and techniques, are essential to maximize the potential of F. oxysporum (UPM31P1) and S. marcescens (UPM39B3) as BCAs against Fusarium wilt of banana.
format Thesis
author Ting, Adeline Su Yien
author_facet Ting, Adeline Su Yien
author_sort Ting, Adeline Su Yien
title Endophytic Microorganisms for Improvement of Banana Vigour and Tolerance to Fusarium Wilt
title_short Endophytic Microorganisms for Improvement of Banana Vigour and Tolerance to Fusarium Wilt
title_full Endophytic Microorganisms for Improvement of Banana Vigour and Tolerance to Fusarium Wilt
title_fullStr Endophytic Microorganisms for Improvement of Banana Vigour and Tolerance to Fusarium Wilt
title_full_unstemmed Endophytic Microorganisms for Improvement of Banana Vigour and Tolerance to Fusarium Wilt
title_sort endophytic microorganisms for improvement of banana vigour and tolerance to fusarium wilt
publishDate 2005
url http://psasir.upm.edu.my/id/eprint/6170/1/FP_2005_9.pdf
http://psasir.upm.edu.my/id/eprint/6170/
_version_ 1781706607569141760
score 13.211869

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