Cover Image

Effect of different concentrations of egg yolk and virgin coconut oil in Tris-based extenders on chilled and frozen-thawed bull semen

The aim of this study was to evaluate the effects of 8% virgin coconut oil (VCO) combined with different percentages of egg yolk in Tris extender on the quality of chilled and frozen-thawed bull semen. A total of 24 ejaculates from four bulls were collected using an electroejaculator. Semen samples...

Full description

Saved in:
Bibliographic Details
Main Authors: A.A., Tarig, H., Wahid, Y., Rosnina, N., Yimer, Goh, Y. M., Baiee, F. H., Mada, Khumran Armiya'u, H., Salman, Ebrahimi, M.
Format: Article
Language:English
Published: Elsevier 2017
Online Access:http://psasir.upm.edu.my/id/eprint/61556/1/Effect%20of%20different%20concentrations%20of%20egg%20yolk%20and%20virgin%20coconut%20oil%20in%20Tris-based%20extenders%20on%20chilled%20and%20frozen-thawed%20bull%20semen.pdf
http://psasir.upm.edu.my/id/eprint/61556/
https://www.sciencedirect.com/science/article/pii/S0378432016306133?via%3Dihub
Tags: Add Tag
No Tags, Be the first to tag this record!
id my.upm.eprints.61556
record_format eprints
spelling my.upm.eprints.615562022-05-25T02:05:22Z http://psasir.upm.edu.my/id/eprint/61556/ Effect of different concentrations of egg yolk and virgin coconut oil in Tris-based extenders on chilled and frozen-thawed bull semen A.A., Tarig H., Wahid Y., Rosnina N., Yimer Goh, Y. M. Baiee, F. H. Mada, Khumran Armiya'u H., Salman Ebrahimi, M. The aim of this study was to evaluate the effects of 8% virgin coconut oil (VCO) combined with different percentages of egg yolk in Tris extender on the quality of chilled and frozen-thawed bull semen. A total of 24 ejaculates from four bulls were collected using an electroejaculator. Semen samples were diluted with 8% VCO in Tris extender which contained different concentrations 0% (control), 4%, 8%, 12%, 16% and 20% egg yolk. The diluted semen samples were divided into two fractions: one was chilled and stored at 4°C until evaluation after 24, 72, and 144h; the second fraction was processed by chilling for 3h at 4°C to equilibrate, then packaged in 0.25ml straws and frozen and stored in liquid nitrogen at -196°C until evaluation after 7 and 14 days. Both chilled and frozen semen samples were then thawed at 37°C and assessed for general motility using computer-assisted semen analysis (CASA), viability, acrosome integrity, and morphology (eosin-nigrosin), membrane integrity (hypo-osmotic swelling test) and lipid peroxidation (thiobarbituric acid-reactive substances (TBARS)). The results indicate treatments with 8%, 12%, 16% and 20% egg yolk with 8% VCO had greater sperm quality (P<0.05) as compared with the control. The treatment with 20% egg yolk had the greatest sperm quality (P<0.05) among the treated groups for both chilled and frozen-thawed semen. In conclusion, the use of 8% VCO combined with 20% egg yolk in a Tris-based extender enhanced the values for chilled and frozen-thawed quality variables of bull sperm. Elsevier 2017 Article PeerReviewed text en http://psasir.upm.edu.my/id/eprint/61556/1/Effect%20of%20different%20concentrations%20of%20egg%20yolk%20and%20virgin%20coconut%20oil%20in%20Tris-based%20extenders%20on%20chilled%20and%20frozen-thawed%20bull%20semen.pdf A.A., Tarig and H., Wahid and Y., Rosnina and N., Yimer and Goh, Y. M. and Baiee, F. H. and Mada, Khumran Armiya'u and H., Salman and Ebrahimi, M. (2017) Effect of different concentrations of egg yolk and virgin coconut oil in Tris-based extenders on chilled and frozen-thawed bull semen. Animal Reproduction Science, 182. 21 - 27. ISSN 0378-4320; ESSN: 1873-2232 https://www.sciencedirect.com/science/article/pii/S0378432016306133?via%3Dihub 10.1016/j.anireprosci.2017.03.024
institution Universiti Putra Malaysia
building UPM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Putra Malaysia
content_source UPM Institutional Repository
url_provider http://psasir.upm.edu.my/
language English
description The aim of this study was to evaluate the effects of 8% virgin coconut oil (VCO) combined with different percentages of egg yolk in Tris extender on the quality of chilled and frozen-thawed bull semen. A total of 24 ejaculates from four bulls were collected using an electroejaculator. Semen samples were diluted with 8% VCO in Tris extender which contained different concentrations 0% (control), 4%, 8%, 12%, 16% and 20% egg yolk. The diluted semen samples were divided into two fractions: one was chilled and stored at 4°C until evaluation after 24, 72, and 144h; the second fraction was processed by chilling for 3h at 4°C to equilibrate, then packaged in 0.25ml straws and frozen and stored in liquid nitrogen at -196°C until evaluation after 7 and 14 days. Both chilled and frozen semen samples were then thawed at 37°C and assessed for general motility using computer-assisted semen analysis (CASA), viability, acrosome integrity, and morphology (eosin-nigrosin), membrane integrity (hypo-osmotic swelling test) and lipid peroxidation (thiobarbituric acid-reactive substances (TBARS)). The results indicate treatments with 8%, 12%, 16% and 20% egg yolk with 8% VCO had greater sperm quality (P<0.05) as compared with the control. The treatment with 20% egg yolk had the greatest sperm quality (P<0.05) among the treated groups for both chilled and frozen-thawed semen. In conclusion, the use of 8% VCO combined with 20% egg yolk in a Tris-based extender enhanced the values for chilled and frozen-thawed quality variables of bull sperm.
format Article
author A.A., Tarig
H., Wahid
Y., Rosnina
N., Yimer
Goh, Y. M.
Baiee, F. H.
Mada, Khumran Armiya'u
H., Salman
Ebrahimi, M.
spellingShingle A.A., Tarig
H., Wahid
Y., Rosnina
N., Yimer
Goh, Y. M.
Baiee, F. H.
Mada, Khumran Armiya'u
H., Salman
Ebrahimi, M.
Effect of different concentrations of egg yolk and virgin coconut oil in Tris-based extenders on chilled and frozen-thawed bull semen
author_facet A.A., Tarig
H., Wahid
Y., Rosnina
N., Yimer
Goh, Y. M.
Baiee, F. H.
Mada, Khumran Armiya'u
H., Salman
Ebrahimi, M.
author_sort A.A., Tarig
title Effect of different concentrations of egg yolk and virgin coconut oil in Tris-based extenders on chilled and frozen-thawed bull semen
title_short Effect of different concentrations of egg yolk and virgin coconut oil in Tris-based extenders on chilled and frozen-thawed bull semen
title_full Effect of different concentrations of egg yolk and virgin coconut oil in Tris-based extenders on chilled and frozen-thawed bull semen
title_fullStr Effect of different concentrations of egg yolk and virgin coconut oil in Tris-based extenders on chilled and frozen-thawed bull semen
title_full_unstemmed Effect of different concentrations of egg yolk and virgin coconut oil in Tris-based extenders on chilled and frozen-thawed bull semen
title_sort effect of different concentrations of egg yolk and virgin coconut oil in tris-based extenders on chilled and frozen-thawed bull semen
publisher Elsevier
publishDate 2017
url http://psasir.upm.edu.my/id/eprint/61556/1/Effect%20of%20different%20concentrations%20of%20egg%20yolk%20and%20virgin%20coconut%20oil%20in%20Tris-based%20extenders%20on%20chilled%20and%20frozen-thawed%20bull%20semen.pdf
http://psasir.upm.edu.my/id/eprint/61556/
https://www.sciencedirect.com/science/article/pii/S0378432016306133?via%3Dihub
_version_ 1734301585571315712
score 13.211869

Similar Items