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Cryo-electron microscopy structure of the Macrobrachium rosenbergii nodavirus capsid at 7 angstroms resolution

White tail disease in the giant freshwater prawn Macrobrachium rosenbergii causes significant economic losses in shrimp farms and hatcheries and poses a threat to food-security in many developing countries. Outbreaks of Macrobrachium rosenbergii nodavirus (MrNV), the causative agent of white tail di...

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Main Authors: Ho, Kok Lian, Kueh, Chare Li, Beh, Poay Ling, Tan, Wen Siang, Bhella, David
Format: Article
Language:English
Published: Nature Research 2017
Online Access:http://psasir.upm.edu.my/id/eprint/61261/1/TAIL.pdf
http://psasir.upm.edu.my/id/eprint/61261/
https://www.nature.com/articles/s41598-017-02292-0
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spelling my.upm.eprints.612612021-07-16T09:54:35Z http://psasir.upm.edu.my/id/eprint/61261/ Cryo-electron microscopy structure of the Macrobrachium rosenbergii nodavirus capsid at 7 angstroms resolution Ho, Kok Lian Kueh, Chare Li Beh, Poay Ling Tan, Wen Siang Bhella, David White tail disease in the giant freshwater prawn Macrobrachium rosenbergii causes significant economic losses in shrimp farms and hatcheries and poses a threat to food-security in many developing countries. Outbreaks of Macrobrachium rosenbergii nodavirus (MrNV), the causative agent of white tail disease (WTD) are associated with up to 100% mortality rates. There are no interventions available to treat or prevent MrNV disease however. Here we show the structure of MrNV virus-like particles (VLPs) produced by recombinant expression of the capsid protein, using cryogenic electron microscopy. Our data show that MrNV VLPs package nucleic acids in a manner reminiscent of other known nodavirus structures. The structure of the capsid however shows striking differences from insect and fish infecting nodaviruses, which have been shown to assemble trimer-clustered T = 3 icosahedral virus particles. MrNV particles have pronounced dimeric blade-shaped spikes extending up to 6 nm from the outer surface of the capsid shell. Our structural analysis supports the assertion that MrNV may belong to a new genus of the Nodaviridae. Moreover, our study provides the first structural view of an important pathogen affecting aquaculture industries across the world. Nature Research 2017 Article PeerReviewed text en http://psasir.upm.edu.my/id/eprint/61261/1/TAIL.pdf Ho, Kok Lian and Kueh, Chare Li and Beh, Poay Ling and Tan, Wen Siang and Bhella, David (2017) Cryo-electron microscopy structure of the Macrobrachium rosenbergii nodavirus capsid at 7 angstroms resolution. Scientific Reports, 7. art. no. 2083. pp. 1-8. ISSN 2045-2322 https://www.nature.com/articles/s41598-017-02292-0 10.1038/s41598-017-02292-0
institution Universiti Putra Malaysia
building UPM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Putra Malaysia
content_source UPM Institutional Repository
url_provider http://psasir.upm.edu.my/
language English
description White tail disease in the giant freshwater prawn Macrobrachium rosenbergii causes significant economic losses in shrimp farms and hatcheries and poses a threat to food-security in many developing countries. Outbreaks of Macrobrachium rosenbergii nodavirus (MrNV), the causative agent of white tail disease (WTD) are associated with up to 100% mortality rates. There are no interventions available to treat or prevent MrNV disease however. Here we show the structure of MrNV virus-like particles (VLPs) produced by recombinant expression of the capsid protein, using cryogenic electron microscopy. Our data show that MrNV VLPs package nucleic acids in a manner reminiscent of other known nodavirus structures. The structure of the capsid however shows striking differences from insect and fish infecting nodaviruses, which have been shown to assemble trimer-clustered T = 3 icosahedral virus particles. MrNV particles have pronounced dimeric blade-shaped spikes extending up to 6 nm from the outer surface of the capsid shell. Our structural analysis supports the assertion that MrNV may belong to a new genus of the Nodaviridae. Moreover, our study provides the first structural view of an important pathogen affecting aquaculture industries across the world.
format Article
author Ho, Kok Lian
Kueh, Chare Li
Beh, Poay Ling
Tan, Wen Siang
Bhella, David
spellingShingle Ho, Kok Lian
Kueh, Chare Li
Beh, Poay Ling
Tan, Wen Siang
Bhella, David
Cryo-electron microscopy structure of the Macrobrachium rosenbergii nodavirus capsid at 7 angstroms resolution
author_facet Ho, Kok Lian
Kueh, Chare Li
Beh, Poay Ling
Tan, Wen Siang
Bhella, David
author_sort Ho, Kok Lian
title Cryo-electron microscopy structure of the Macrobrachium rosenbergii nodavirus capsid at 7 angstroms resolution
title_short Cryo-electron microscopy structure of the Macrobrachium rosenbergii nodavirus capsid at 7 angstroms resolution
title_full Cryo-electron microscopy structure of the Macrobrachium rosenbergii nodavirus capsid at 7 angstroms resolution
title_fullStr Cryo-electron microscopy structure of the Macrobrachium rosenbergii nodavirus capsid at 7 angstroms resolution
title_full_unstemmed Cryo-electron microscopy structure of the Macrobrachium rosenbergii nodavirus capsid at 7 angstroms resolution
title_sort cryo-electron microscopy structure of the macrobrachium rosenbergii nodavirus capsid at 7 angstroms resolution
publisher Nature Research
publishDate 2017
url http://psasir.upm.edu.my/id/eprint/61261/1/TAIL.pdf
http://psasir.upm.edu.my/id/eprint/61261/
https://www.nature.com/articles/s41598-017-02292-0
_version_ 1706958775638818816
score 13.211869

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