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Comparative characterization of murine Bone marrow mesenchymal stem cells cultured using two different supplements

Preclinical studies on mesenchymal stem cells (MSC) have allowed the cells to be considered as a promising candidate for cellular therapy. The mouse is the most widely used species for studying the characteristics of MSC. In recent years, conflicting data were reported regarding growth kinetics, su...

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Main Authors: Lye, Kwan Liang, Sulaiman, Nurfazilahyusnitafitrah, Vidyadaran, Sharmili, Nordin, Norshariza, Karuppiah, Thilakavathy
Format: Article
Language:English
Published: BioMedPress 2017
Online Access:http://psasir.upm.edu.my/id/eprint/61158/1/Comparative%20characterization%20of%20murine%20Bone%20marrow%20mesenchymal%20stem%20cells%20cultured%20using%20two%20different%20supplements.pdf
http://psasir.upm.edu.my/id/eprint/61158/
http://www.bmrat.org/index.php/BMRAT/article/view/233
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spelling my.upm.eprints.611582019-05-14T01:24:46Z http://psasir.upm.edu.my/id/eprint/61158/ Comparative characterization of murine Bone marrow mesenchymal stem cells cultured using two different supplements Lye, Kwan Liang Sulaiman, Nurfazilahyusnitafitrah Vidyadaran, Sharmili Nordin, Norshariza Karuppiah, Thilakavathy Preclinical studies on mesenchymal stem cells (MSC) have allowed the cells to be considered as a promising candidate for cellular therapy. The mouse is the most widely used species for studying the characteristics of MSC. In recent years, conflicting data were reported regarding growth kinetics, surface marker profile, differentiation capacity, genetic instability or malignant transformation and so forth, that may be a result of a range of factors. One of the factors probably is the culture medium formulation. Here we have made a comparative characterization of bone marrow-derived mesenchymal stem cells (mBM-MSC), under the same experimental conditions, cultured using two common supplements, fetal bovine serum (FBS) and MesenCultTM Stimulatory Supplement (MSS). mBM-MSC isolated from the tibias of C57BL/6 mice were cultured and expanded in Dulbecco’s Modified Eagle’s Medium supplemented with either 15% FBS or 15% MSS. Clonogenic potential, population doubling time, immunophenotyping, differentiation immunosuppression potentials and chromosome analysis of early and late passage of mBM-MSC were assessed. The findings showed that the immunophenotype and differentiation potential of mBMMSC were similar when cultured using these supplements irrespective of passages. Variations were seen in clonogenic, growth, proliferation rate and immunosuppression potential of the mBM-MSC. This study also revealed that prolonged culture will disrupt their genetic stability regardless of the supplements used. The genetically mutated mBM-MSC were also found to maintain their stemness characteristics and immunosuppression potential. In conclusion, culture medium formulation causes variations in the cultured MSC and may influence downstream investigation findings. BioMedPress 2017 Article PeerReviewed text en http://psasir.upm.edu.my/id/eprint/61158/1/Comparative%20characterization%20of%20murine%20Bone%20marrow%20mesenchymal%20stem%20cells%20cultured%20using%20two%20different%20supplements.pdf Lye, Kwan Liang and Sulaiman, Nurfazilahyusnitafitrah and Vidyadaran, Sharmili and Nordin, Norshariza and Karuppiah, Thilakavathy (2017) Comparative characterization of murine Bone marrow mesenchymal stem cells cultured using two different supplements. Biomedical Research and Therapy, 4. S25. ISSN 2198-4093 http://www.bmrat.org/index.php/BMRAT/article/view/233 10.15419/bmrat.v4iS.233
institution Universiti Putra Malaysia
building UPM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Putra Malaysia
content_source UPM Institutional Repository
url_provider http://psasir.upm.edu.my/
language English
description Preclinical studies on mesenchymal stem cells (MSC) have allowed the cells to be considered as a promising candidate for cellular therapy. The mouse is the most widely used species for studying the characteristics of MSC. In recent years, conflicting data were reported regarding growth kinetics, surface marker profile, differentiation capacity, genetic instability or malignant transformation and so forth, that may be a result of a range of factors. One of the factors probably is the culture medium formulation. Here we have made a comparative characterization of bone marrow-derived mesenchymal stem cells (mBM-MSC), under the same experimental conditions, cultured using two common supplements, fetal bovine serum (FBS) and MesenCultTM Stimulatory Supplement (MSS). mBM-MSC isolated from the tibias of C57BL/6 mice were cultured and expanded in Dulbecco’s Modified Eagle’s Medium supplemented with either 15% FBS or 15% MSS. Clonogenic potential, population doubling time, immunophenotyping, differentiation immunosuppression potentials and chromosome analysis of early and late passage of mBM-MSC were assessed. The findings showed that the immunophenotype and differentiation potential of mBMMSC were similar when cultured using these supplements irrespective of passages. Variations were seen in clonogenic, growth, proliferation rate and immunosuppression potential of the mBM-MSC. This study also revealed that prolonged culture will disrupt their genetic stability regardless of the supplements used. The genetically mutated mBM-MSC were also found to maintain their stemness characteristics and immunosuppression potential. In conclusion, culture medium formulation causes variations in the cultured MSC and may influence downstream investigation findings.
format Article
author Lye, Kwan Liang
Sulaiman, Nurfazilahyusnitafitrah
Vidyadaran, Sharmili
Nordin, Norshariza
Karuppiah, Thilakavathy
spellingShingle Lye, Kwan Liang
Sulaiman, Nurfazilahyusnitafitrah
Vidyadaran, Sharmili
Nordin, Norshariza
Karuppiah, Thilakavathy
Comparative characterization of murine Bone marrow mesenchymal stem cells cultured using two different supplements
author_facet Lye, Kwan Liang
Sulaiman, Nurfazilahyusnitafitrah
Vidyadaran, Sharmili
Nordin, Norshariza
Karuppiah, Thilakavathy
author_sort Lye, Kwan Liang
title Comparative characterization of murine Bone marrow mesenchymal stem cells cultured using two different supplements
title_short Comparative characterization of murine Bone marrow mesenchymal stem cells cultured using two different supplements
title_full Comparative characterization of murine Bone marrow mesenchymal stem cells cultured using two different supplements
title_fullStr Comparative characterization of murine Bone marrow mesenchymal stem cells cultured using two different supplements
title_full_unstemmed Comparative characterization of murine Bone marrow mesenchymal stem cells cultured using two different supplements
title_sort comparative characterization of murine bone marrow mesenchymal stem cells cultured using two different supplements
publisher BioMedPress
publishDate 2017
url http://psasir.upm.edu.my/id/eprint/61158/1/Comparative%20characterization%20of%20murine%20Bone%20marrow%20mesenchymal%20stem%20cells%20cultured%20using%20two%20different%20supplements.pdf
http://psasir.upm.edu.my/id/eprint/61158/
http://www.bmrat.org/index.php/BMRAT/article/view/233
_version_ 1643837518175535104
score 13.211869

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