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Biosensing of hepatitis B antigen with poly(acrylic acid) hydrogel immobilized with antigens and antibodies

Hydrogel based on poly(acrylic acid) (PAAc) polymers was successfully fabricated as the biosensor for detecting hepatitis B core antigen (HBcAg). Specifically, the pendant HBcAg and the anti-hepatitis B core antigen (anti-HBc) antibody were first immobilized on the PAAc, which were then covalently c...

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Main Authors: Swee, Lu Lim, Ooi, Chien-Wei, Tan, Wen Siang, Chan, Eng-Seng, Ho, Kok Lian, Tey, Beng Ti
Format: Article
Language:English
Published: Elsevier 2017
Online Access:http://psasir.upm.edu.my/id/eprint/61006/1/Biosensing%20of%20hepatitis%20B%20antigen%20with%20poly%28acrylic%20acid%29%20hydrogel%20immobilized%20with%20antigens%20and%20antibodies.pdf
http://psasir.upm.edu.my/id/eprint/61006/
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spelling my.upm.eprints.610062019-04-30T04:07:20Z http://psasir.upm.edu.my/id/eprint/61006/ Biosensing of hepatitis B antigen with poly(acrylic acid) hydrogel immobilized with antigens and antibodies Swee, Lu Lim Ooi, Chien-Wei Tan, Wen Siang Chan, Eng-Seng Ho, Kok Lian Tey, Beng Ti Hydrogel based on poly(acrylic acid) (PAAc) polymers was successfully fabricated as the biosensor for detecting hepatitis B core antigen (HBcAg). Specifically, the pendant HBcAg and the anti-hepatitis B core antigen (anti-HBc) antibody were first immobilized on the PAAc, which were then covalently cross-linked via radical polymerization to form the HBcAg-sensitive (HBPAAc) hydrogel. The non-covalent affinity binding between the immobilized HBcAg and anti-HBc would be disrupted by the presence of free HBcAg in the HBPAAc hydrogel. The competitive binding of free HBcAg on the immobilized anti-HBc triggered the swelling of HBPAAc hydrogel. The equilibrium swelling ratio and the oscillatory swelling-deswelling kinetics of the HBPAAc hydrogel in response to protein concentration were studied. The swelling ratio of HBPAAc hydrogel increased along with an increase in HBcAg concentration until equilibrium was achieved at 4 mg/mL HBcAg. The HBPAAc hydrogel did not exhibit swelling/deswelling behavior when interacted with the negative control, i.e., bovine serum albumin (BSA). Based on the result of oscillatory swelling-deswelling of HBPAAc hydrogel, the minimum duration for HBcAg detection by the HBPAAc hydrogel was ∼20 min, whereas the regeneration of HBPAAc hydrogel took about 60 min. These results confirmed the reversibility and the reusability of this HBcAg-sensing HBPAAc hydrogel. Besides, the hydrogel demonstrated zero cross-reactivity to the hepatitis B surface antigen, a common serological marker for hepatitis B patients. The high sensitivity of HBPAAc hydrogel to the HBcAg was successfully demonstrated with quartz crystal microgravimetry. The magnitude of resonance frequency was inversely correlated with the swelling of HBPAAc hydrogel, which is governed by the concentration of analyte HBcAg. The application of HBPAAc hydrogel as a biosensor component in the detection and surveillance of hepatitis B holds great promises. Elsevier 2017 Article PeerReviewed text en http://psasir.upm.edu.my/id/eprint/61006/1/Biosensing%20of%20hepatitis%20B%20antigen%20with%20poly%28acrylic%20acid%29%20hydrogel%20immobilized%20with%20antigens%20and%20antibodies.pdf Swee, Lu Lim and Ooi, Chien-Wei and Tan, Wen Siang and Chan, Eng-Seng and Ho, Kok Lian and Tey, Beng Ti (2017) Biosensing of hepatitis B antigen with poly(acrylic acid) hydrogel immobilized with antigens and antibodies. Sensors and Actuators B: Chemical, 252. pp. 409-417. ISSN 0925-4005; ESSN: 1873-3077 https://reader.elsevier.com/reader/sd/pii/S0925400517310183?token=2C09CA9BB38BE27140A4D8D20CEA8AC2ECE04214985C949D656774DD1271A8B78819B950D66BC4ADAC13C158C7528FFE 10.1016/j.snb.2017.06.003
institution Universiti Putra Malaysia
building UPM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Putra Malaysia
content_source UPM Institutional Repository
url_provider http://psasir.upm.edu.my/
language English
description Hydrogel based on poly(acrylic acid) (PAAc) polymers was successfully fabricated as the biosensor for detecting hepatitis B core antigen (HBcAg). Specifically, the pendant HBcAg and the anti-hepatitis B core antigen (anti-HBc) antibody were first immobilized on the PAAc, which were then covalently cross-linked via radical polymerization to form the HBcAg-sensitive (HBPAAc) hydrogel. The non-covalent affinity binding between the immobilized HBcAg and anti-HBc would be disrupted by the presence of free HBcAg in the HBPAAc hydrogel. The competitive binding of free HBcAg on the immobilized anti-HBc triggered the swelling of HBPAAc hydrogel. The equilibrium swelling ratio and the oscillatory swelling-deswelling kinetics of the HBPAAc hydrogel in response to protein concentration were studied. The swelling ratio of HBPAAc hydrogel increased along with an increase in HBcAg concentration until equilibrium was achieved at 4 mg/mL HBcAg. The HBPAAc hydrogel did not exhibit swelling/deswelling behavior when interacted with the negative control, i.e., bovine serum albumin (BSA). Based on the result of oscillatory swelling-deswelling of HBPAAc hydrogel, the minimum duration for HBcAg detection by the HBPAAc hydrogel was ∼20 min, whereas the regeneration of HBPAAc hydrogel took about 60 min. These results confirmed the reversibility and the reusability of this HBcAg-sensing HBPAAc hydrogel. Besides, the hydrogel demonstrated zero cross-reactivity to the hepatitis B surface antigen, a common serological marker for hepatitis B patients. The high sensitivity of HBPAAc hydrogel to the HBcAg was successfully demonstrated with quartz crystal microgravimetry. The magnitude of resonance frequency was inversely correlated with the swelling of HBPAAc hydrogel, which is governed by the concentration of analyte HBcAg. The application of HBPAAc hydrogel as a biosensor component in the detection and surveillance of hepatitis B holds great promises.
format Article
author Swee, Lu Lim
Ooi, Chien-Wei
Tan, Wen Siang
Chan, Eng-Seng
Ho, Kok Lian
Tey, Beng Ti
spellingShingle Swee, Lu Lim
Ooi, Chien-Wei
Tan, Wen Siang
Chan, Eng-Seng
Ho, Kok Lian
Tey, Beng Ti
Biosensing of hepatitis B antigen with poly(acrylic acid) hydrogel immobilized with antigens and antibodies
author_facet Swee, Lu Lim
Ooi, Chien-Wei
Tan, Wen Siang
Chan, Eng-Seng
Ho, Kok Lian
Tey, Beng Ti
author_sort Swee, Lu Lim
title Biosensing of hepatitis B antigen with poly(acrylic acid) hydrogel immobilized with antigens and antibodies
title_short Biosensing of hepatitis B antigen with poly(acrylic acid) hydrogel immobilized with antigens and antibodies
title_full Biosensing of hepatitis B antigen with poly(acrylic acid) hydrogel immobilized with antigens and antibodies
title_fullStr Biosensing of hepatitis B antigen with poly(acrylic acid) hydrogel immobilized with antigens and antibodies
title_full_unstemmed Biosensing of hepatitis B antigen with poly(acrylic acid) hydrogel immobilized with antigens and antibodies
title_sort biosensing of hepatitis b antigen with poly(acrylic acid) hydrogel immobilized with antigens and antibodies
publisher Elsevier
publishDate 2017
url http://psasir.upm.edu.my/id/eprint/61006/1/Biosensing%20of%20hepatitis%20B%20antigen%20with%20poly%28acrylic%20acid%29%20hydrogel%20immobilized%20with%20antigens%20and%20antibodies.pdf
http://psasir.upm.edu.my/id/eprint/61006/
https://reader.elsevier.com/reader/sd/pii/S0925400517310183?token=2C09CA9BB38BE27140A4D8D20CEA8AC2ECE04214985C949D656774DD1271A8B78819B950D66BC4ADAC13C158C7528FFE
_version_ 1643837485069893632
score 13.211869

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