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Antioxidant-mediated response of a susceptible papaya cultivar to a compatible strain of Erwinia mallotivora

Dieback disease caused by Erwinia mallotivora is the most devastating disease of papaya in Malaysia. Most papaya cultivars here are susceptible to the disease, indicating a compatible relationship between the cultivars and the pathogenic strains of the bacteria. In this study, we evaluated early res...

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Main Authors: Supian, Suhaina, Saidi, Noor Baity, Chien, Yeong Wee, Abdullah, Mohd Puad
Format: Article
Language:English
Published: Academic Press 2017
Online Access:http://psasir.upm.edu.my/id/eprint/60898/1/Antioxidant-mediated%20response%20of%20a%20susceptible%20papaya%20cultivar%20to%20a%20compatible%20strain%20of%20Erwinia%20mallotivora.pdf
http://psasir.upm.edu.my/id/eprint/60898/
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spelling my.upm.eprints.608982019-03-25T01:07:43Z http://psasir.upm.edu.my/id/eprint/60898/ Antioxidant-mediated response of a susceptible papaya cultivar to a compatible strain of Erwinia mallotivora Supian, Suhaina Saidi, Noor Baity Chien, Yeong Wee Abdullah, Mohd Puad Dieback disease caused by Erwinia mallotivora is the most devastating disease of papaya in Malaysia. Most papaya cultivars here are susceptible to the disease, indicating a compatible relationship between the cultivars and the pathogenic strains of the bacteria. In this study, we evaluated early responses of a susceptible cultivar to a virulent strain of the bacteria by monitoring the disease symptoms, measuring the expression patterns and activities of the oxidative enzymes superoxide dismutase (SOD) and peroxidase (POD), and profiling the leaf proteome. Water-soaked lesions and necrotic spots were evidenced on the inoculated leaves at 6 days post-inoculation (dpi) which were deteriorated rapidly with death at 12 dpi. The two oxidative enzymes were responsive to the infection as shown by their induced enzymatic activities and gene expressions in the leaves of the infected plants. The 2D-PAGE profile of the leaf proteome exhibited a significant increase in the intensity of the SOD spot, confirming its involvement in the response. In addition, differential expressions of the protein spots known to be related to stress response, energy production and photosynthesis provided insights into the plant's global response to the infection. Overall, this study suggested that the early response to E. mallotivora inoculation in the susceptible papaya cultivar was likely to be mediated by the antioxidant defense system but the effectiveness of the defense could have been compromised with the down-regulation of important proteins required for normal plant growth and development. This study provides the basis for studying the defect in the defense mechanism that turned this cultivar susceptible to the infection. Academic Press 2017 Article PeerReviewed text en http://psasir.upm.edu.my/id/eprint/60898/1/Antioxidant-mediated%20response%20of%20a%20susceptible%20papaya%20cultivar%20to%20a%20compatible%20strain%20of%20Erwinia%20mallotivora.pdf Supian, Suhaina and Saidi, Noor Baity and Chien, Yeong Wee and Abdullah, Mohd Puad (2017) Antioxidant-mediated response of a susceptible papaya cultivar to a compatible strain of Erwinia mallotivora. Physiological and Molecular Plant Pathology, 98. 37 - 45. ISSN 0885-5765; ESSN: 1096-1178 https://pdf.sciencedirectassets.com/272405/1-s2.0-S0885576517X00027/1-s2.0-S0885576516300753/main.pdf?x-amz-security-token=FQoGZXIvYXdzEDEaDPX0C6%2FYHEpi9kKfByK3A5WPNqg1nKuFFGHcHYAXEFXfOIGJz6a0dqUPOJfi3uH75AatjVemwHm5JrNAwyIP4%2BWqE77s6wsYvsmB1ONJPTzwwcuL 10.1016/j.pmpp.2017.02.005
institution Universiti Putra Malaysia
building UPM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Putra Malaysia
content_source UPM Institutional Repository
url_provider http://psasir.upm.edu.my/
language English
description Dieback disease caused by Erwinia mallotivora is the most devastating disease of papaya in Malaysia. Most papaya cultivars here are susceptible to the disease, indicating a compatible relationship between the cultivars and the pathogenic strains of the bacteria. In this study, we evaluated early responses of a susceptible cultivar to a virulent strain of the bacteria by monitoring the disease symptoms, measuring the expression patterns and activities of the oxidative enzymes superoxide dismutase (SOD) and peroxidase (POD), and profiling the leaf proteome. Water-soaked lesions and necrotic spots were evidenced on the inoculated leaves at 6 days post-inoculation (dpi) which were deteriorated rapidly with death at 12 dpi. The two oxidative enzymes were responsive to the infection as shown by their induced enzymatic activities and gene expressions in the leaves of the infected plants. The 2D-PAGE profile of the leaf proteome exhibited a significant increase in the intensity of the SOD spot, confirming its involvement in the response. In addition, differential expressions of the protein spots known to be related to stress response, energy production and photosynthesis provided insights into the plant's global response to the infection. Overall, this study suggested that the early response to E. mallotivora inoculation in the susceptible papaya cultivar was likely to be mediated by the antioxidant defense system but the effectiveness of the defense could have been compromised with the down-regulation of important proteins required for normal plant growth and development. This study provides the basis for studying the defect in the defense mechanism that turned this cultivar susceptible to the infection.
format Article
author Supian, Suhaina
Saidi, Noor Baity
Chien, Yeong Wee
Abdullah, Mohd Puad
spellingShingle Supian, Suhaina
Saidi, Noor Baity
Chien, Yeong Wee
Abdullah, Mohd Puad
Antioxidant-mediated response of a susceptible papaya cultivar to a compatible strain of Erwinia mallotivora
author_facet Supian, Suhaina
Saidi, Noor Baity
Chien, Yeong Wee
Abdullah, Mohd Puad
author_sort Supian, Suhaina
title Antioxidant-mediated response of a susceptible papaya cultivar to a compatible strain of Erwinia mallotivora
title_short Antioxidant-mediated response of a susceptible papaya cultivar to a compatible strain of Erwinia mallotivora
title_full Antioxidant-mediated response of a susceptible papaya cultivar to a compatible strain of Erwinia mallotivora
title_fullStr Antioxidant-mediated response of a susceptible papaya cultivar to a compatible strain of Erwinia mallotivora
title_full_unstemmed Antioxidant-mediated response of a susceptible papaya cultivar to a compatible strain of Erwinia mallotivora
title_sort antioxidant-mediated response of a susceptible papaya cultivar to a compatible strain of erwinia mallotivora
publisher Academic Press
publishDate 2017
url http://psasir.upm.edu.my/id/eprint/60898/1/Antioxidant-mediated%20response%20of%20a%20susceptible%20papaya%20cultivar%20to%20a%20compatible%20strain%20of%20Erwinia%20mallotivora.pdf
http://psasir.upm.edu.my/id/eprint/60898/
https://pdf.sciencedirectassets.com/272405/1-s2.0-S0885576517X00027/1-s2.0-S0885576516300753/main.pdf?x-amz-security-token=FQoGZXIvYXdzEDEaDPX0C6%2FYHEpi9kKfByK3A5WPNqg1nKuFFGHcHYAXEFXfOIGJz6a0dqUPOJfi3uH75AatjVemwHm5JrNAwyIP4%2BWqE77s6wsYvsmB1ONJPTzwwcuL
_version_ 1643837460523778048
score 13.211869

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