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Actinobacteria from Greenwich Island and Dee Island: isolation, diversity and distribution

Actinobacteria from underexplored and unusual environments have gained significant attention for their capability in producing novel bioactive molecules of diverse chemical entities. Streptomyces is the most prolific Actinobacteria in producing useful molecules. Rapid decline effectiveness of existi...

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Main Authors: Pek, Lim Chu, Chai, Hoon Khoo, Yoke, Kqueen Cheah
Format: Article
Language:English
Published: Biome Scientia Sdn Bhd 2017
Online Access:http://psasir.upm.edu.my/id/eprint/60760/1/Actinobacteria%20from%20Greenwich%20Island%20and%20Dee%20Island%20isolation%2C%20diversity%20and%20distribution.pdf
http://psasir.upm.edu.my/id/eprint/60760/
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spelling my.upm.eprints.607602019-04-24T14:23:10Z http://psasir.upm.edu.my/id/eprint/60760/ Actinobacteria from Greenwich Island and Dee Island: isolation, diversity and distribution Pek, Lim Chu Chai, Hoon Khoo Yoke, Kqueen Cheah Actinobacteria from underexplored and unusual environments have gained significant attention for their capability in producing novel bioactive molecules of diverse chemical entities. Streptomyces is the most prolific Actinobacteria in producing useful molecules. Rapid decline effectiveness of existing antibiotics in the treatment of infections are caused by the emergence of multidrug-resistant pathogens. Intensive efforts are urgently required in isolating non-Streptomyces or rare Actinobacteria and understanding of their distribution in the harsh environment for new drug discovery. In this study, pretreatment of soil samples with 1.5% phenol was used for the selective isolation of Actinobacteria from Dee Island and Greenwich Island. A high number of non-Streptomyces (69.4%) or rare Actinobacteria was significantly recovered despite the Streptomyces (30.6%), including the genera Micromonospora, Micrococcus, Kocuria, Dermacoccus, Brachybacterium, Brevibacterium, Rhodococcus, Microbacterium and Rothia. Reduced diversity and shift of distribution were observed at the elevated level of soil pH. The members of genera Streptomyces, Micromonospora and Micrococcus were found to distribute and tolerate to a relatively high pH level of soil (pH 9.4-9.5), and could potentially be alkaliphilic Actinobacteria. The phylogenetic analysis had revealed some potentially new taxa members of the genera Micromonospora, Micrococcus and Rhodococcus. Principal Component Analysis of soil samples was used to uncover the factors that underlie the diversity of culturable Actinobacteria. Water availability in soil was examined as the principal factor that shaped the diversity of the Actinobacteria, by providing a dynamic source for microbial interactions and elevated diversity of Actinobacteria. Biome Scientia Sdn Bhd 2017 Article PeerReviewed text en http://psasir.upm.edu.my/id/eprint/60760/1/Actinobacteria%20from%20Greenwich%20Island%20and%20Dee%20Island%20isolation%2C%20diversity%20and%20distribution.pdf Pek, Lim Chu and Chai, Hoon Khoo and Yoke, Kqueen Cheah (2017) Actinobacteria from Greenwich Island and Dee Island: isolation, diversity and distribution. Life Sciences, Medicine and Biomedicine, 1 (1). pp. 1-17. ISSN 2600-7207 file:///C:/Users/upm1/Desktop/2-Article,%20Main%20Manuscript%20Document-21-5-10-20180205.pdf 10.28916/lsmb.1.1.2017.2
institution Universiti Putra Malaysia
building UPM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Putra Malaysia
content_source UPM Institutional Repository
url_provider http://psasir.upm.edu.my/
language English
description Actinobacteria from underexplored and unusual environments have gained significant attention for their capability in producing novel bioactive molecules of diverse chemical entities. Streptomyces is the most prolific Actinobacteria in producing useful molecules. Rapid decline effectiveness of existing antibiotics in the treatment of infections are caused by the emergence of multidrug-resistant pathogens. Intensive efforts are urgently required in isolating non-Streptomyces or rare Actinobacteria and understanding of their distribution in the harsh environment for new drug discovery. In this study, pretreatment of soil samples with 1.5% phenol was used for the selective isolation of Actinobacteria from Dee Island and Greenwich Island. A high number of non-Streptomyces (69.4%) or rare Actinobacteria was significantly recovered despite the Streptomyces (30.6%), including the genera Micromonospora, Micrococcus, Kocuria, Dermacoccus, Brachybacterium, Brevibacterium, Rhodococcus, Microbacterium and Rothia. Reduced diversity and shift of distribution were observed at the elevated level of soil pH. The members of genera Streptomyces, Micromonospora and Micrococcus were found to distribute and tolerate to a relatively high pH level of soil (pH 9.4-9.5), and could potentially be alkaliphilic Actinobacteria. The phylogenetic analysis had revealed some potentially new taxa members of the genera Micromonospora, Micrococcus and Rhodococcus. Principal Component Analysis of soil samples was used to uncover the factors that underlie the diversity of culturable Actinobacteria. Water availability in soil was examined as the principal factor that shaped the diversity of the Actinobacteria, by providing a dynamic source for microbial interactions and elevated diversity of Actinobacteria.
format Article
author Pek, Lim Chu
Chai, Hoon Khoo
Yoke, Kqueen Cheah
spellingShingle Pek, Lim Chu
Chai, Hoon Khoo
Yoke, Kqueen Cheah
Actinobacteria from Greenwich Island and Dee Island: isolation, diversity and distribution
author_facet Pek, Lim Chu
Chai, Hoon Khoo
Yoke, Kqueen Cheah
author_sort Pek, Lim Chu
title Actinobacteria from Greenwich Island and Dee Island: isolation, diversity and distribution
title_short Actinobacteria from Greenwich Island and Dee Island: isolation, diversity and distribution
title_full Actinobacteria from Greenwich Island and Dee Island: isolation, diversity and distribution
title_fullStr Actinobacteria from Greenwich Island and Dee Island: isolation, diversity and distribution
title_full_unstemmed Actinobacteria from Greenwich Island and Dee Island: isolation, diversity and distribution
title_sort actinobacteria from greenwich island and dee island: isolation, diversity and distribution
publisher Biome Scientia Sdn Bhd
publishDate 2017
url http://psasir.upm.edu.my/id/eprint/60760/1/Actinobacteria%20from%20Greenwich%20Island%20and%20Dee%20Island%20isolation%2C%20diversity%20and%20distribution.pdf
http://psasir.upm.edu.my/id/eprint/60760/
_version_ 1643837433280724992
score 13.211869

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