Cover Image

A simple add-and display method for immobilisation of cancer drug on his-tagged virus-like nanoparticles for controlled drug delivery

pH-responsive virus-like nanoparticles (VLNPs) hold promising potential as drug delivery systems for cancer therapy. In the present study, hepatitis B virus (HBV) VLNPs harbouring His-tags were used to display doxorubicin (DOX) via nitrilotriacetic acid (NTA) conjugation. The His-tags served as pH-r...

Full description

Saved in:
Bibliographic Details
Main Authors: Biabanikhankahdani, Roya, Bayat, Saadi, Ho, Kok Lian, Mohammed Alitheen, Noorjahan Banu, Tan, Wen Siang
Format: Article
Language:English
Published: Nature Publishing Group 2017
Online Access:http://psasir.upm.edu.my/id/eprint/60732/1/A%20simple%20add-and%20display%20method%20for%20immobilisation%20of%20cancer%20drug%20on%20his-tagged%20virus-like%20nanoparticles%20for%20controlled%20drug%20delivery.pdf
http://psasir.upm.edu.my/id/eprint/60732/
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5509718/pdf/41598_2017_Article_5525.pdf
Tags: Add Tag
No Tags, Be the first to tag this record!
id my.upm.eprints.60732
record_format eprints
spelling my.upm.eprints.607322019-03-28T04:22:19Z http://psasir.upm.edu.my/id/eprint/60732/ A simple add-and display method for immobilisation of cancer drug on his-tagged virus-like nanoparticles for controlled drug delivery Biabanikhankahdani, Roya Bayat, Saadi Ho, Kok Lian Mohammed Alitheen, Noorjahan Banu Tan, Wen Siang pH-responsive virus-like nanoparticles (VLNPs) hold promising potential as drug delivery systems for cancer therapy. In the present study, hepatitis B virus (HBV) VLNPs harbouring His-tags were used to display doxorubicin (DOX) via nitrilotriacetic acid (NTA) conjugation. The His-tags served as pH-responsive nanojoints which released DOX from VLNPs in a controlled manner. The His-tagged VLNPs conjugated non-covalently with NTA-DOX, and cross-linked with folic acid (FA) were able to specifically target and deliver the DOX into ovarian cancer cells via folate receptor (FR)-mediated endocytosis. The cytotoxicity and cellular uptake results revealed that the His-tagged VLNPs significantly increased the accumulation of DOX in the ovarian cancer cells and enhanced the uptake of DOX, which improved anti-tumour effects. This study demonstrated that NTA-DOX can be easily displayed on His-tagged VLNPs by a simple Add-and-Display step with high coupling efficiency and the drug was only released at low pH in a controlled manner. This approach facilitates specific attachment of any drug molecule on His-tagged VLNPs at the very mild conditions without changing the biological structure and native conformation of the VLNPs. Nature Publishing Group 2017 Article PeerReviewed text en http://psasir.upm.edu.my/id/eprint/60732/1/A%20simple%20add-and%20display%20method%20for%20immobilisation%20of%20cancer%20drug%20on%20his-tagged%20virus-like%20nanoparticles%20for%20controlled%20drug%20delivery.pdf Biabanikhankahdani, Roya and Bayat, Saadi and Ho, Kok Lian and Mohammed Alitheen, Noorjahan Banu and Tan, Wen Siang (2017) A simple add-and display method for immobilisation of cancer drug on his-tagged virus-like nanoparticles for controlled drug delivery. Scientific Reports, 7. pp. 1-12. ISSN 2045-2322 https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5509718/pdf/41598_2017_Article_5525.pdf 10.1038/s41598-017-05525-4
institution Universiti Putra Malaysia
building UPM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Putra Malaysia
content_source UPM Institutional Repository
url_provider http://psasir.upm.edu.my/
language English
description pH-responsive virus-like nanoparticles (VLNPs) hold promising potential as drug delivery systems for cancer therapy. In the present study, hepatitis B virus (HBV) VLNPs harbouring His-tags were used to display doxorubicin (DOX) via nitrilotriacetic acid (NTA) conjugation. The His-tags served as pH-responsive nanojoints which released DOX from VLNPs in a controlled manner. The His-tagged VLNPs conjugated non-covalently with NTA-DOX, and cross-linked with folic acid (FA) were able to specifically target and deliver the DOX into ovarian cancer cells via folate receptor (FR)-mediated endocytosis. The cytotoxicity and cellular uptake results revealed that the His-tagged VLNPs significantly increased the accumulation of DOX in the ovarian cancer cells and enhanced the uptake of DOX, which improved anti-tumour effects. This study demonstrated that NTA-DOX can be easily displayed on His-tagged VLNPs by a simple Add-and-Display step with high coupling efficiency and the drug was only released at low pH in a controlled manner. This approach facilitates specific attachment of any drug molecule on His-tagged VLNPs at the very mild conditions without changing the biological structure and native conformation of the VLNPs.
format Article
author Biabanikhankahdani, Roya
Bayat, Saadi
Ho, Kok Lian
Mohammed Alitheen, Noorjahan Banu
Tan, Wen Siang
spellingShingle Biabanikhankahdani, Roya
Bayat, Saadi
Ho, Kok Lian
Mohammed Alitheen, Noorjahan Banu
Tan, Wen Siang
A simple add-and display method for immobilisation of cancer drug on his-tagged virus-like nanoparticles for controlled drug delivery
author_facet Biabanikhankahdani, Roya
Bayat, Saadi
Ho, Kok Lian
Mohammed Alitheen, Noorjahan Banu
Tan, Wen Siang
author_sort Biabanikhankahdani, Roya
title A simple add-and display method for immobilisation of cancer drug on his-tagged virus-like nanoparticles for controlled drug delivery
title_short A simple add-and display method for immobilisation of cancer drug on his-tagged virus-like nanoparticles for controlled drug delivery
title_full A simple add-and display method for immobilisation of cancer drug on his-tagged virus-like nanoparticles for controlled drug delivery
title_fullStr A simple add-and display method for immobilisation of cancer drug on his-tagged virus-like nanoparticles for controlled drug delivery
title_full_unstemmed A simple add-and display method for immobilisation of cancer drug on his-tagged virus-like nanoparticles for controlled drug delivery
title_sort simple add-and display method for immobilisation of cancer drug on his-tagged virus-like nanoparticles for controlled drug delivery
publisher Nature Publishing Group
publishDate 2017
url http://psasir.upm.edu.my/id/eprint/60732/1/A%20simple%20add-and%20display%20method%20for%20immobilisation%20of%20cancer%20drug%20on%20his-tagged%20virus-like%20nanoparticles%20for%20controlled%20drug%20delivery.pdf
http://psasir.upm.edu.my/id/eprint/60732/
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5509718/pdf/41598_2017_Article_5525.pdf
_version_ 1643837427022823424
score 13.211869

Similar Items