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Characterisation and pathological variability of Exserohilum turcicum responsible for causing northern corn leaf blight (NCLB) disease in Malaysia

Aims: Corn is grown primarily for human consumption. It is considered as the second most important cereal crop after rice in Asia. Many diseases affect this crop due to planting of susceptible hybrids. This research is aimed to characterize the causative agent of northern corn leaf blight disease in...

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Main Authors: Kutawa, Abdulaziz Bashir, Sijam, Kamaruzaman, Ahmad, Khairulmazmi, Ahmad Seman, Zulkifli, Ab Razak, Mohd Shahril Firdaus, Abdullah, Norzihan
Format: Article
Language:English
Published: Malaysian Society for Microbiology 2017
Online Access:http://psasir.upm.edu.my/id/eprint/60602/1/Characterisation%20and%20pathological%20variability%20of%20Exserohilum%20turcicum%20responsible%20for%20causing%20northern%20corn%20leaf%20blight%20%28NCLB%29%20disease%20in%20Malaysia.pdf
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spelling my.upm.eprints.606022018-06-08T00:25:38Z http://psasir.upm.edu.my/id/eprint/60602/ Characterisation and pathological variability of Exserohilum turcicum responsible for causing northern corn leaf blight (NCLB) disease in Malaysia Kutawa, Abdulaziz Bashir Sijam, Kamaruzaman Ahmad, Khairulmazmi Ahmad Seman, Zulkifli Ab Razak, Mohd Shahril Firdaus Abdullah, Norzihan Aims: Corn is grown primarily for human consumption. It is considered as the second most important cereal crop after rice in Asia. Many diseases affect this crop due to planting of susceptible hybrids. This research is aimed to characterize the causative agent of northern corn leaf blight disease in Malaysia, caused by Exserohilum turcicum. Methodology and results: Leaf samples were collected from infected farms of 2 corn growing areas of Peninsular Malaysia in 2015. A total of 5 fungal isolates were examined for cultural, morphological and molecular properties, and based on the results, the five isolates were identified as E. turcicum. The conidial shapes were observed to be elongated and spindle. Cultural characteristics showed that variation existed among the isolates in colony growth and colour. Mycelia growth rates of the isolates were significantly different on potato dextrose agar (PDA), corn meal agar (CMA) and potato sucrose agar (PSA) media, growth on CMA was faster than on PSA and PDA. The isolates were grouped into three groups based on colony colour i.e. light gray, gray and dark gray. The isolates were categorized into 2 groups based on growth namely, moderate growth and profused growth. The number of septa ranged from 5-7 to 7-10 representing isolates ET002 and ET003, respectively. Similarly, conidial length varied from 56.7 μm to 89.44 μm for isolates ET002 and ET003, respectively. The pathogenic variability tested on Thai Super Sweet (TSS) corn variety, showed that isolates ET001 and ET003 were more aggressive while isolate ET005 was less aggressive among the isolates tested. Conclusion, significance and impact of the study: Both morphology and molecular results showed that, the isolates were identified as E. turcicum. The findings of this study will serve as a baseline for future studies and will help to minimize losses in yield. Malaysian Society for Microbiology 2017 Article PeerReviewed text en http://psasir.upm.edu.my/id/eprint/60602/1/Characterisation%20and%20pathological%20variability%20of%20Exserohilum%20turcicum%20responsible%20for%20causing%20northern%20corn%20leaf%20blight%20%28NCLB%29%20disease%20in%20Malaysia.pdf Kutawa, Abdulaziz Bashir and Sijam, Kamaruzaman and Ahmad, Khairulmazmi and Ahmad Seman, Zulkifli and Ab Razak, Mohd Shahril Firdaus and Abdullah, Norzihan (2017) Characterisation and pathological variability of Exserohilum turcicum responsible for causing northern corn leaf blight (NCLB) disease in Malaysia. Malaysian Journal of Microbiology, 13 (1). pp. 41-49. ISSN 1823-8262; ESSN: 2231-7538 http://mjm.usm.my/index.php?r=cms/entry/view&id=51
institution Universiti Putra Malaysia
building UPM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Putra Malaysia
content_source UPM Institutional Repository
url_provider http://psasir.upm.edu.my/
language English
description Aims: Corn is grown primarily for human consumption. It is considered as the second most important cereal crop after rice in Asia. Many diseases affect this crop due to planting of susceptible hybrids. This research is aimed to characterize the causative agent of northern corn leaf blight disease in Malaysia, caused by Exserohilum turcicum. Methodology and results: Leaf samples were collected from infected farms of 2 corn growing areas of Peninsular Malaysia in 2015. A total of 5 fungal isolates were examined for cultural, morphological and molecular properties, and based on the results, the five isolates were identified as E. turcicum. The conidial shapes were observed to be elongated and spindle. Cultural characteristics showed that variation existed among the isolates in colony growth and colour. Mycelia growth rates of the isolates were significantly different on potato dextrose agar (PDA), corn meal agar (CMA) and potato sucrose agar (PSA) media, growth on CMA was faster than on PSA and PDA. The isolates were grouped into three groups based on colony colour i.e. light gray, gray and dark gray. The isolates were categorized into 2 groups based on growth namely, moderate growth and profused growth. The number of septa ranged from 5-7 to 7-10 representing isolates ET002 and ET003, respectively. Similarly, conidial length varied from 56.7 μm to 89.44 μm for isolates ET002 and ET003, respectively. The pathogenic variability tested on Thai Super Sweet (TSS) corn variety, showed that isolates ET001 and ET003 were more aggressive while isolate ET005 was less aggressive among the isolates tested. Conclusion, significance and impact of the study: Both morphology and molecular results showed that, the isolates were identified as E. turcicum. The findings of this study will serve as a baseline for future studies and will help to minimize losses in yield.
format Article
author Kutawa, Abdulaziz Bashir
Sijam, Kamaruzaman
Ahmad, Khairulmazmi
Ahmad Seman, Zulkifli
Ab Razak, Mohd Shahril Firdaus
Abdullah, Norzihan
spellingShingle Kutawa, Abdulaziz Bashir
Sijam, Kamaruzaman
Ahmad, Khairulmazmi
Ahmad Seman, Zulkifli
Ab Razak, Mohd Shahril Firdaus
Abdullah, Norzihan
Characterisation and pathological variability of Exserohilum turcicum responsible for causing northern corn leaf blight (NCLB) disease in Malaysia
author_facet Kutawa, Abdulaziz Bashir
Sijam, Kamaruzaman
Ahmad, Khairulmazmi
Ahmad Seman, Zulkifli
Ab Razak, Mohd Shahril Firdaus
Abdullah, Norzihan
author_sort Kutawa, Abdulaziz Bashir
title Characterisation and pathological variability of Exserohilum turcicum responsible for causing northern corn leaf blight (NCLB) disease in Malaysia
title_short Characterisation and pathological variability of Exserohilum turcicum responsible for causing northern corn leaf blight (NCLB) disease in Malaysia
title_full Characterisation and pathological variability of Exserohilum turcicum responsible for causing northern corn leaf blight (NCLB) disease in Malaysia
title_fullStr Characterisation and pathological variability of Exserohilum turcicum responsible for causing northern corn leaf blight (NCLB) disease in Malaysia
title_full_unstemmed Characterisation and pathological variability of Exserohilum turcicum responsible for causing northern corn leaf blight (NCLB) disease in Malaysia
title_sort characterisation and pathological variability of exserohilum turcicum responsible for causing northern corn leaf blight (nclb) disease in malaysia
publisher Malaysian Society for Microbiology
publishDate 2017
url http://psasir.upm.edu.my/id/eprint/60602/1/Characterisation%20and%20pathological%20variability%20of%20Exserohilum%20turcicum%20responsible%20for%20causing%20northern%20corn%20leaf%20blight%20%28NCLB%29%20disease%20in%20Malaysia.pdf
http://psasir.upm.edu.my/id/eprint/60602/
http://mjm.usm.my/index.php?r=cms/entry/view&id=51
_version_ 1643837407144968192
score 13.211869

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