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Semipurified ethyl acetate partition of methanolic extract of Melastoma malabathricum leaves exerts gastroprotective activity partly via its antioxidant-antisecretory-anti-inflammatory action and synergistic action of several flavonoid-based compounds

Recent study has demonstrated the gastroprotective activity of crude methanolic extract of M. malabathricum leaves. The present study evaluated the gastroprotective potential of semipurified extracts (partitions): petroleum ether, ethyl acetate (EAMM), and aqueous obtained from the methanolic extrac...

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Main Authors: Ismail Suhaimy, Noor Wahida, Noor Azmi, Ahmad Khusairi, Mohtarrudin, Norhafizah, Omar, Maizatul Hasyima, Md Tohid, Siti Farah, Cheema, Manraj Singh, Teh, Lay Kek, Salleh, Mohd Zaki, Zakaria, Zainul Amiruddin
Format: Article
Language:English
Published: Hindawi Publishing Corporation 2017
Online Access:http://psasir.upm.edu.my/id/eprint/60579/1/60579.pdf
http://psasir.upm.edu.my/id/eprint/60579/
https://www.hindawi.com/journals/omcl/2017/6542631/abs/
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spelling my.upm.eprints.605792018-06-07T08:42:03Z http://psasir.upm.edu.my/id/eprint/60579/ Semipurified ethyl acetate partition of methanolic extract of Melastoma malabathricum leaves exerts gastroprotective activity partly via its antioxidant-antisecretory-anti-inflammatory action and synergistic action of several flavonoid-based compounds Ismail Suhaimy, Noor Wahida Noor Azmi, Ahmad Khusairi Mohtarrudin, Norhafizah Omar, Maizatul Hasyima Md Tohid, Siti Farah Cheema, Manraj Singh Teh, Lay Kek Salleh, Mohd Zaki Zakaria, Zainul Amiruddin Recent study has demonstrated the gastroprotective activity of crude methanolic extract of M. malabathricum leaves. The present study evaluated the gastroprotective potential of semipurified extracts (partitions): petroleum ether, ethyl acetate (EAMM), and aqueous obtained from the methanolic extract followed by the elucidation of the gastroprotective mechanisms of the most effective partition. Using the ethanol-induced gastric ulcer assay, all partitions exerted significant gastroprotection, with EAMM being the most effective partition. EAMM significantly (i) reduced the volume and acidity (free and total) while increasing the pH of gastric juice and enhanced the gastric wall mucus secretion when assessed using the pylorus ligation assay, (ii) increased the enzymatic and nonenzymatic antioxidant activity of the stomach tissue, (iii) lost its gastroprotective activity following pretreatment with N-omega-nitro-L-arginine methyl ester (L-NAME; NO blocker) or carbenoxolone (CBXN; NP-SH blocker), (iv) exerted antioxidant activity against various in vitro oxidation assays, and (v) showed moderate in vitro anti-inflammatory activity via the LOX-modulated pathway. In conclusion, EAMM exerts a remarkable NO/NP-SH-dependent gastroprotective effect that is attributed to its antisecretory and antioxidant activities, ability to stimulate the gastric mucus production and endogenous antioxidant system, and synergistic action of several gastroprotective-induced flavonoids. Hindawi Publishing Corporation 2017 Article PeerReviewed text en http://psasir.upm.edu.my/id/eprint/60579/1/60579.pdf Ismail Suhaimy, Noor Wahida and Noor Azmi, Ahmad Khusairi and Mohtarrudin, Norhafizah and Omar, Maizatul Hasyima and Md Tohid, Siti Farah and Cheema, Manraj Singh and Teh, Lay Kek and Salleh, Mohd Zaki and Zakaria, Zainul Amiruddin (2017) Semipurified ethyl acetate partition of methanolic extract of Melastoma malabathricum leaves exerts gastroprotective activity partly via its antioxidant-antisecretory-anti-inflammatory action and synergistic action of several flavonoid-based compounds. Oxidative Medicine and Cellular Longevity, 2017. art. no. 6542631. pp. 1-14. ISSN 1942-0900; ESSN: 1942-0994 https://www.hindawi.com/journals/omcl/2017/6542631/abs/ 10.1155/2017/6542631
institution Universiti Putra Malaysia
building UPM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Putra Malaysia
content_source UPM Institutional Repository
url_provider http://psasir.upm.edu.my/
language English
description Recent study has demonstrated the gastroprotective activity of crude methanolic extract of M. malabathricum leaves. The present study evaluated the gastroprotective potential of semipurified extracts (partitions): petroleum ether, ethyl acetate (EAMM), and aqueous obtained from the methanolic extract followed by the elucidation of the gastroprotective mechanisms of the most effective partition. Using the ethanol-induced gastric ulcer assay, all partitions exerted significant gastroprotection, with EAMM being the most effective partition. EAMM significantly (i) reduced the volume and acidity (free and total) while increasing the pH of gastric juice and enhanced the gastric wall mucus secretion when assessed using the pylorus ligation assay, (ii) increased the enzymatic and nonenzymatic antioxidant activity of the stomach tissue, (iii) lost its gastroprotective activity following pretreatment with N-omega-nitro-L-arginine methyl ester (L-NAME; NO blocker) or carbenoxolone (CBXN; NP-SH blocker), (iv) exerted antioxidant activity against various in vitro oxidation assays, and (v) showed moderate in vitro anti-inflammatory activity via the LOX-modulated pathway. In conclusion, EAMM exerts a remarkable NO/NP-SH-dependent gastroprotective effect that is attributed to its antisecretory and antioxidant activities, ability to stimulate the gastric mucus production and endogenous antioxidant system, and synergistic action of several gastroprotective-induced flavonoids.
format Article
author Ismail Suhaimy, Noor Wahida
Noor Azmi, Ahmad Khusairi
Mohtarrudin, Norhafizah
Omar, Maizatul Hasyima
Md Tohid, Siti Farah
Cheema, Manraj Singh
Teh, Lay Kek
Salleh, Mohd Zaki
Zakaria, Zainul Amiruddin
spellingShingle Ismail Suhaimy, Noor Wahida
Noor Azmi, Ahmad Khusairi
Mohtarrudin, Norhafizah
Omar, Maizatul Hasyima
Md Tohid, Siti Farah
Cheema, Manraj Singh
Teh, Lay Kek
Salleh, Mohd Zaki
Zakaria, Zainul Amiruddin
Semipurified ethyl acetate partition of methanolic extract of Melastoma malabathricum leaves exerts gastroprotective activity partly via its antioxidant-antisecretory-anti-inflammatory action and synergistic action of several flavonoid-based compounds
author_facet Ismail Suhaimy, Noor Wahida
Noor Azmi, Ahmad Khusairi
Mohtarrudin, Norhafizah
Omar, Maizatul Hasyima
Md Tohid, Siti Farah
Cheema, Manraj Singh
Teh, Lay Kek
Salleh, Mohd Zaki
Zakaria, Zainul Amiruddin
author_sort Ismail Suhaimy, Noor Wahida
title Semipurified ethyl acetate partition of methanolic extract of Melastoma malabathricum leaves exerts gastroprotective activity partly via its antioxidant-antisecretory-anti-inflammatory action and synergistic action of several flavonoid-based compounds
title_short Semipurified ethyl acetate partition of methanolic extract of Melastoma malabathricum leaves exerts gastroprotective activity partly via its antioxidant-antisecretory-anti-inflammatory action and synergistic action of several flavonoid-based compounds
title_full Semipurified ethyl acetate partition of methanolic extract of Melastoma malabathricum leaves exerts gastroprotective activity partly via its antioxidant-antisecretory-anti-inflammatory action and synergistic action of several flavonoid-based compounds
title_fullStr Semipurified ethyl acetate partition of methanolic extract of Melastoma malabathricum leaves exerts gastroprotective activity partly via its antioxidant-antisecretory-anti-inflammatory action and synergistic action of several flavonoid-based compounds
title_full_unstemmed Semipurified ethyl acetate partition of methanolic extract of Melastoma malabathricum leaves exerts gastroprotective activity partly via its antioxidant-antisecretory-anti-inflammatory action and synergistic action of several flavonoid-based compounds
title_sort semipurified ethyl acetate partition of methanolic extract of melastoma malabathricum leaves exerts gastroprotective activity partly via its antioxidant-antisecretory-anti-inflammatory action and synergistic action of several flavonoid-based compounds
publisher Hindawi Publishing Corporation
publishDate 2017
url http://psasir.upm.edu.my/id/eprint/60579/1/60579.pdf
http://psasir.upm.edu.my/id/eprint/60579/
https://www.hindawi.com/journals/omcl/2017/6542631/abs/
_version_ 1643837400242192384
score 13.211869

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