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Isolation and pathogenicity of Streptococcus iniae in cultured red hybrid tilapia in Malaysia

This study describes the isolation and pathogenicity of Streptococcus iniae in cultured red hybrid tilapia (Nile Tilapia Oreochromis niloticus × Mozambique Tilapia O. mossambicus) in Malaysia. The isolated gram-positive S. iniae appeared punctiform, transparently white, catalase and oxidase negative...

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Main Authors: Rahmatullah, Muhammad, Ariff, M., Kahieshesfandiari, M., Mohd Daud, Hassan, Saad, Mohd Zamri, Mohd Yusoff, Md Sabri, Azmai, Mohammad Noor Amal, Md Yasin, Ina Salwany
Format: Article
Language:English
Published: American Fisheries Society 2017
Online Access:http://psasir.upm.edu.my/id/eprint/59615/1/Isolation%20and%20pathogenicity%20of%20Streptococcus%20iniae%20in%20cultured%20red%20hybrid%20tilapia%20in%20Malaysia.pdf
http://psasir.upm.edu.my/id/eprint/59615/
http://onlinelibrary.wiley.com/doi/10.1080/08997659.2017.1360411/abstract
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spelling my.upm.eprints.596152018-03-13T08:12:35Z http://psasir.upm.edu.my/id/eprint/59615/ Isolation and pathogenicity of Streptococcus iniae in cultured red hybrid tilapia in Malaysia Rahmatullah, Muhammad Ariff, M. Kahieshesfandiari, M. Mohd Daud, Hassan Saad, Mohd Zamri Mohd Yusoff, Md Sabri Azmai, Mohammad Noor Amal Md Yasin, Ina Salwany This study describes the isolation and pathogenicity of Streptococcus iniae in cultured red hybrid tilapia (Nile Tilapia Oreochromis niloticus × Mozambique Tilapia O. mossambicus) in Malaysia. The isolated gram-positive S. iniae appeared punctiform, transparently white, catalase and oxidase negative and produced complete β-hemolysis on blood agar, while a PCR assay resulted in the amplification of the 16 S rRNA gene and lactate oxidase encoded genes. The isolate was sensitive to tetracycline, vancomycin, and bacitracin but was resistant to streptomycin, ampicillin, penicillin, and erythromycin. Pathogenicity trials conducted in local red hybrid tilapia (mean ± SE = 20.00 ± 0.45 g) showed 90.0, 96.7, and 100.0% mortality within 14 d postinfection following intraperitoneal exposure to 104, 106, and 108 CFU/mL of the pathogen, respectively. The clinical signs included erratic swimming, lethargy, and inappetance at 6 h postinfection, while mortality was recorded at less than 24 h postinfection in all infected groups. The LD50-336 h of S. iniae against the red hybrid tilapia was 102 CFU/mL. The post mortem examinations revealed congested livers, kidneys, and spleens of the infected fish. This is the first report of S. iniae experimental infection in cultured red hybrid tilapia in Malaysia. American Fisheries Society 2017 Article PeerReviewed text en http://psasir.upm.edu.my/id/eprint/59615/1/Isolation%20and%20pathogenicity%20of%20Streptococcus%20iniae%20in%20cultured%20red%20hybrid%20tilapia%20in%20Malaysia.pdf Rahmatullah, Muhammad and Ariff, M. and Kahieshesfandiari, M. and Mohd Daud, Hassan and Saad, Mohd Zamri and Mohd Yusoff, Md Sabri and Azmai, Mohammad Noor Amal and Md Yasin, Ina Salwany (2017) Isolation and pathogenicity of Streptococcus iniae in cultured red hybrid tilapia in Malaysia. Journal of Aquatic Animal Health, 29 (4). pp. 208-213. ISSN 0899-7659; ESSN: 1548-8667 http://onlinelibrary.wiley.com/doi/10.1080/08997659.2017.1360411/abstract 10.1080/08997659.2017.1360411
institution Universiti Putra Malaysia
building UPM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Putra Malaysia
content_source UPM Institutional Repository
url_provider http://psasir.upm.edu.my/
language English
description This study describes the isolation and pathogenicity of Streptococcus iniae in cultured red hybrid tilapia (Nile Tilapia Oreochromis niloticus × Mozambique Tilapia O. mossambicus) in Malaysia. The isolated gram-positive S. iniae appeared punctiform, transparently white, catalase and oxidase negative and produced complete β-hemolysis on blood agar, while a PCR assay resulted in the amplification of the 16 S rRNA gene and lactate oxidase encoded genes. The isolate was sensitive to tetracycline, vancomycin, and bacitracin but was resistant to streptomycin, ampicillin, penicillin, and erythromycin. Pathogenicity trials conducted in local red hybrid tilapia (mean ± SE = 20.00 ± 0.45 g) showed 90.0, 96.7, and 100.0% mortality within 14 d postinfection following intraperitoneal exposure to 104, 106, and 108 CFU/mL of the pathogen, respectively. The clinical signs included erratic swimming, lethargy, and inappetance at 6 h postinfection, while mortality was recorded at less than 24 h postinfection in all infected groups. The LD50-336 h of S. iniae against the red hybrid tilapia was 102 CFU/mL. The post mortem examinations revealed congested livers, kidneys, and spleens of the infected fish. This is the first report of S. iniae experimental infection in cultured red hybrid tilapia in Malaysia.
format Article
author Rahmatullah, Muhammad
Ariff, M.
Kahieshesfandiari, M.
Mohd Daud, Hassan
Saad, Mohd Zamri
Mohd Yusoff, Md Sabri
Azmai, Mohammad Noor Amal
Md Yasin, Ina Salwany
spellingShingle Rahmatullah, Muhammad
Ariff, M.
Kahieshesfandiari, M.
Mohd Daud, Hassan
Saad, Mohd Zamri
Mohd Yusoff, Md Sabri
Azmai, Mohammad Noor Amal
Md Yasin, Ina Salwany
Isolation and pathogenicity of Streptococcus iniae in cultured red hybrid tilapia in Malaysia
author_facet Rahmatullah, Muhammad
Ariff, M.
Kahieshesfandiari, M.
Mohd Daud, Hassan
Saad, Mohd Zamri
Mohd Yusoff, Md Sabri
Azmai, Mohammad Noor Amal
Md Yasin, Ina Salwany
author_sort Rahmatullah, Muhammad
title Isolation and pathogenicity of Streptococcus iniae in cultured red hybrid tilapia in Malaysia
title_short Isolation and pathogenicity of Streptococcus iniae in cultured red hybrid tilapia in Malaysia
title_full Isolation and pathogenicity of Streptococcus iniae in cultured red hybrid tilapia in Malaysia
title_fullStr Isolation and pathogenicity of Streptococcus iniae in cultured red hybrid tilapia in Malaysia
title_full_unstemmed Isolation and pathogenicity of Streptococcus iniae in cultured red hybrid tilapia in Malaysia
title_sort isolation and pathogenicity of streptococcus iniae in cultured red hybrid tilapia in malaysia
publisher American Fisheries Society
publishDate 2017
url http://psasir.upm.edu.my/id/eprint/59615/1/Isolation%20and%20pathogenicity%20of%20Streptococcus%20iniae%20in%20cultured%20red%20hybrid%20tilapia%20in%20Malaysia.pdf
http://psasir.upm.edu.my/id/eprint/59615/
http://onlinelibrary.wiley.com/doi/10.1080/08997659.2017.1360411/abstract
_version_ 1643837124151083008
score 13.211869

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