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Assessment of the genetic variation of Malaysian durian varieties using inter-simple sequence repeat markers and chloroplast DNA sequences

To date, 124 durian varieties have been registered with the Malaysian Department of Agriculture based on phenotypic characteristics. However, the levels and patterns of genetic variation among the varieties are still unknown. In this study, the leaves of 27 durian varieties were sampled from four du...

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Main Authors: Siew, Ging Yang, Ng, Wei Lun, Salleh, Muhammad Fadzly, Tan, Sheau Wei, Ky, Huynh, Mohammed Alitheen, Noorjahan Banu, Tan, Soon Guan, Yeap, Swee Keong
Format: Article
Language:English
Published: Universiti Putra Malaysia Press 2018
Online Access:http://psasir.upm.edu.my/id/eprint/59609/1/21%20JTAS-1110-2017-5thProof.pdf
http://psasir.upm.edu.my/id/eprint/59609/
http://www.pertanika.upm.edu.my/Pertanika%20PAPERS/JTAS%20Vol.%2041%20(1)%20Feb.%202018/21%20JTAS-1110-2017-5thProof.pdf
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spelling my.upm.eprints.596092018-03-13T00:44:09Z http://psasir.upm.edu.my/id/eprint/59609/ Assessment of the genetic variation of Malaysian durian varieties using inter-simple sequence repeat markers and chloroplast DNA sequences Siew, Ging Yang Ng, Wei Lun Salleh, Muhammad Fadzly Tan, Sheau Wei Ky, Huynh Mohammed Alitheen, Noorjahan Banu Tan, Soon Guan Yeap, Swee Keong To date, 124 durian varieties have been registered with the Malaysian Department of Agriculture based on phenotypic characteristics. However, the levels and patterns of genetic variation among the varieties are still unknown. In this study, the leaves of 27 durian varieties were sampled from four durian orchards in Universiti Putra Malaysia, namely Bukit Ekspo, Putra Mart, Ladang Puchong and Ladang 5. Twenty five inter-simple sequence repeat (ISSR) primers were tested for PCR amplification on DNA samples. Twelve ISSR primers amplified 133 clear and reproducible DNA fragments and 122 (91.73%) were polymorphic, indicating a high level of genetic variation among these durian varieties. Primers flanking four chloroplast DNA (cpDNA) regions (trnL-trnF, atpB-rbcL and trnH-psbA intergenic spacers as well as the partial matK gene) were tested for PCR amplification. Two cpDNA regions (trnL-trnF and matK) were successfully amplified, but showed no variation in their DNA sequences, even when additional samples from Vietnam were included. The findings in this preliminary study lay a foundation for more comprehensive future studies on the genetic variation among durian varieties. Universiti Putra Malaysia Press 2018 Article PeerReviewed text en http://psasir.upm.edu.my/id/eprint/59609/1/21%20JTAS-1110-2017-5thProof.pdf Siew, Ging Yang and Ng, Wei Lun and Salleh, Muhammad Fadzly and Tan, Sheau Wei and Ky, Huynh and Mohammed Alitheen, Noorjahan Banu and Tan, Soon Guan and Yeap, Swee Keong (2018) Assessment of the genetic variation of Malaysian durian varieties using inter-simple sequence repeat markers and chloroplast DNA sequences. Pertanika Journal of Tropical Agricultural Science, 41 (1). pp. 321-332. ISSN 1511-3701; ESSN: 2231-8542 http://www.pertanika.upm.edu.my/Pertanika%20PAPERS/JTAS%20Vol.%2041%20(1)%20Feb.%202018/21%20JTAS-1110-2017-5thProof.pdf
institution Universiti Putra Malaysia
building UPM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Putra Malaysia
content_source UPM Institutional Repository
url_provider http://psasir.upm.edu.my/
language English
description To date, 124 durian varieties have been registered with the Malaysian Department of Agriculture based on phenotypic characteristics. However, the levels and patterns of genetic variation among the varieties are still unknown. In this study, the leaves of 27 durian varieties were sampled from four durian orchards in Universiti Putra Malaysia, namely Bukit Ekspo, Putra Mart, Ladang Puchong and Ladang 5. Twenty five inter-simple sequence repeat (ISSR) primers were tested for PCR amplification on DNA samples. Twelve ISSR primers amplified 133 clear and reproducible DNA fragments and 122 (91.73%) were polymorphic, indicating a high level of genetic variation among these durian varieties. Primers flanking four chloroplast DNA (cpDNA) regions (trnL-trnF, atpB-rbcL and trnH-psbA intergenic spacers as well as the partial matK gene) were tested for PCR amplification. Two cpDNA regions (trnL-trnF and matK) were successfully amplified, but showed no variation in their DNA sequences, even when additional samples from Vietnam were included. The findings in this preliminary study lay a foundation for more comprehensive future studies on the genetic variation among durian varieties.
format Article
author Siew, Ging Yang
Ng, Wei Lun
Salleh, Muhammad Fadzly
Tan, Sheau Wei
Ky, Huynh
Mohammed Alitheen, Noorjahan Banu
Tan, Soon Guan
Yeap, Swee Keong
spellingShingle Siew, Ging Yang
Ng, Wei Lun
Salleh, Muhammad Fadzly
Tan, Sheau Wei
Ky, Huynh
Mohammed Alitheen, Noorjahan Banu
Tan, Soon Guan
Yeap, Swee Keong
Assessment of the genetic variation of Malaysian durian varieties using inter-simple sequence repeat markers and chloroplast DNA sequences
author_facet Siew, Ging Yang
Ng, Wei Lun
Salleh, Muhammad Fadzly
Tan, Sheau Wei
Ky, Huynh
Mohammed Alitheen, Noorjahan Banu
Tan, Soon Guan
Yeap, Swee Keong
author_sort Siew, Ging Yang
title Assessment of the genetic variation of Malaysian durian varieties using inter-simple sequence repeat markers and chloroplast DNA sequences
title_short Assessment of the genetic variation of Malaysian durian varieties using inter-simple sequence repeat markers and chloroplast DNA sequences
title_full Assessment of the genetic variation of Malaysian durian varieties using inter-simple sequence repeat markers and chloroplast DNA sequences
title_fullStr Assessment of the genetic variation of Malaysian durian varieties using inter-simple sequence repeat markers and chloroplast DNA sequences
title_full_unstemmed Assessment of the genetic variation of Malaysian durian varieties using inter-simple sequence repeat markers and chloroplast DNA sequences
title_sort assessment of the genetic variation of malaysian durian varieties using inter-simple sequence repeat markers and chloroplast dna sequences
publisher Universiti Putra Malaysia Press
publishDate 2018
url http://psasir.upm.edu.my/id/eprint/59609/1/21%20JTAS-1110-2017-5thProof.pdf
http://psasir.upm.edu.my/id/eprint/59609/
http://www.pertanika.upm.edu.my/Pertanika%20PAPERS/JTAS%20Vol.%2041%20(1)%20Feb.%202018/21%20JTAS-1110-2017-5thProof.pdf
_version_ 1643837122334949376
score 13.211869

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