Electrical characterization of GO at different pH towards MCF7 and MCF10a: preliminary result
The intracellular pH of cancerous cell is commonly acidic while the intracellular pH of normal cell is neutral. The objective of this study is to study the electrical characterization in terms of resistance between the pH of sensing material with the intracellular pH of the cells. Three different pH...
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2017
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my.upm.eprints.594812018-03-07T02:36:34Z http://psasir.upm.edu.my/id/eprint/59481/ Electrical characterization of GO at different pH towards MCF7 and MCF10a: preliminary result Mazlan, N. S. Mat Isa, Siti Salwa Ramli, Muhammad Mahyiddin Talip, L. F. A. Che Halin, Dewi Suriyani Mohamed, Rafeezul Mohtar, Mohd Nazim The intracellular pH of cancerous cell is commonly acidic while the intracellular pH of normal cell is neutral. The objective of this study is to study the electrical characterization in terms of resistance between the pH of sensing material with the intracellular pH of the cells. Three different pH of Graphene Oxide (GO) were used as a solvent to analyze their interaction towards breast cancer cells (MCF7) and breast normal cells (MCF10a). GO which produced by Hummer's method was used due to their solubility and biocompatibility characteristics which easily diffuse through the cell. In this experiment, the characteristics of GO were analyzed and confirmed by using Atomic Force Microscopy (AFM) and Fourier Transform Infrared spectroscopy (FTIR). In order to measure the resistance of MCF7 and MCF10a cells after treated with GO for 24 hours, gold electrodes with 10 μ-gaps of interdigitated electrodes (IDEs) were used. The results were obtained for three periods of time which were immediate, 5 minutes and 10 minutes after the treated cells being exposed at room temperature. The results show that the resistance of MCF10a cells increased after treated with higher pH of GO which is pH 7 and the resistances of the MCF7 cells decreased as the pH of GO increased to pH 7. Finally, the viable cells were calculated by using haemocytometer in order to prove that the increased of the resistances were due to the increased number of viable cells. IEEE 2017 Conference or Workshop Item PeerReviewed text en http://psasir.upm.edu.my/id/eprint/59481/1/Electrical%20characterization%20of%20GO%20at%20different%20pH%20towards%20MCF7%20and%20MCF10a%20preliminary%20result.pdf Mazlan, N. S. and Mat Isa, Siti Salwa and Ramli, Muhammad Mahyiddin and Talip, L. F. A. and Che Halin, Dewi Suriyani and Mohamed, Rafeezul and Mohtar, Mohd Nazim (2017) Electrical characterization of GO at different pH towards MCF7 and MCF10a: preliminary result. In: 2017 IEEE Asia Pacific Conference on Postgraduate Research in Microelectronics and Electronics (PrimeAsia), 31 Oct.-2 Nov. 2017, Kuala Lumpur, Malaysia. (pp. 89-92). 10.1109/PRIMEASIA.2017.8280371 |
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The intracellular pH of cancerous cell is commonly acidic while the intracellular pH of normal cell is neutral. The objective of this study is to study the electrical characterization in terms of resistance between the pH of sensing material with the intracellular pH of the cells. Three different pH of Graphene Oxide (GO) were used as a solvent to analyze their interaction towards breast cancer cells (MCF7) and breast normal cells (MCF10a). GO which produced by Hummer's method was used due to their solubility and biocompatibility characteristics which easily diffuse through the cell. In this experiment, the characteristics of GO were analyzed and confirmed by using Atomic Force Microscopy (AFM) and Fourier Transform Infrared spectroscopy (FTIR). In order to measure the resistance of MCF7 and MCF10a cells after treated with GO for 24 hours, gold electrodes with 10 μ-gaps of interdigitated electrodes (IDEs) were used. The results were obtained for three periods of time which were immediate, 5 minutes and 10 minutes after the treated cells being exposed at room temperature. The results show that the resistance of MCF10a cells increased after treated with higher pH of GO which is pH 7 and the resistances of the MCF7 cells decreased as the pH of GO increased to pH 7. Finally, the viable cells were calculated by using haemocytometer in order to prove that the increased of the resistances were due to the increased number of viable cells. |
| format |
Conference or Workshop Item |
| author |
Mazlan, N. S. Mat Isa, Siti Salwa Ramli, Muhammad Mahyiddin Talip, L. F. A. Che Halin, Dewi Suriyani Mohamed, Rafeezul Mohtar, Mohd Nazim |
| spellingShingle |
Mazlan, N. S. Mat Isa, Siti Salwa Ramli, Muhammad Mahyiddin Talip, L. F. A. Che Halin, Dewi Suriyani Mohamed, Rafeezul Mohtar, Mohd Nazim Electrical characterization of GO at different pH towards MCF7 and MCF10a: preliminary result |
| author_facet |
Mazlan, N. S. Mat Isa, Siti Salwa Ramli, Muhammad Mahyiddin Talip, L. F. A. Che Halin, Dewi Suriyani Mohamed, Rafeezul Mohtar, Mohd Nazim |
| author_sort |
Mazlan, N. S. |
| title |
Electrical characterization of GO at different pH towards MCF7 and MCF10a: preliminary result |
| title_short |
Electrical characterization of GO at different pH towards MCF7 and MCF10a: preliminary result |
| title_full |
Electrical characterization of GO at different pH towards MCF7 and MCF10a: preliminary result |
| title_fullStr |
Electrical characterization of GO at different pH towards MCF7 and MCF10a: preliminary result |
| title_full_unstemmed |
Electrical characterization of GO at different pH towards MCF7 and MCF10a: preliminary result |
| title_sort |
electrical characterization of go at different ph towards mcf7 and mcf10a: preliminary result |
| publisher |
IEEE |
| publishDate |
2017 |
| url |
http://psasir.upm.edu.my/id/eprint/59481/1/Electrical%20characterization%20of%20GO%20at%20different%20pH%20towards%20MCF7%20and%20MCF10a%20preliminary%20result.pdf http://psasir.upm.edu.my/id/eprint/59481/ |
| _version_ |
1643837085215358976 |
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13.211869 |